Results 91 to 100 of about 2,869,940 (329)

Analysis of the regulation of undecaprenyl diphosphate dephosphorylation in Escherichia coli

open access: yesFEBS Open Bio, EarlyView.
BacA, PgpB, and YbjG phosphatases are involved in undecaprenyl phosphate (C55P) synthesis in Escherichia coli. We analyzed the lipid contents and the gene expression in the gene‐disruption strains. Undecaprenyl diphosphate (C55PP) level increased in the bacA, ybjG double‐disruption strain, but C55P levels were similar in all strains.
Tomotaka Jitsukawa   +2 more
wiley   +1 more source

Ribonucleolytic resection is required for repair of strand displaced nonhomologous end-joining intermediates [PDF]

open access: yes, 2013
Nonhomologous end-joining (NHEJ) pathways repair DNA double-strand breaks (DSBs) in eukaryotes and many prokaryotes, although it is not reported to operate in the third domain of life, archaea. Here, we describe a complete NHEJ complex, consisting of DNA
Bartlett, Edward   +2 more
core   +1 more source

Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies

open access: yesNucleic Acids Research, 2012
16S ribosomal RNA gene (rDNA) amplicon analysis remains the standard approach for the cultivation-independent investigation of microbial diversity. The accuracy of these analyses depends strongly on the choice of primers.
A. Klindworth   +6 more
semanticscholar   +1 more source

The amazing world of bacterial structured RNAs [PDF]

open access: yesGenome Biology, 2010
The discovery of several new structured non-coding RNAs in bacterial and archaeal genomes and metagenomes raises burning questions about their biological and biochemical functions.
openaire   +3 more sources

Subcellular localization of a bacterial regulatory RNA [PDF]

open access: yesProceedings of the National Academy of Sciences, 2009
Eukaryotes and bacteria regulate the activity of some proteins by localizing them to discrete subcellular structures, and eukaryotes localize some RNAs for the same purpose. To explore whether bacteria also spatially regulate RNAs, the localization of tmRNA was determined using fluorescence in situ hybridization ...
Jay H. Russell, Kenneth C. Keiler
openaire   +3 more sources

Characterization of the thermophilic xylanase Fsa02490Xyn from the hyperthermophile Fervidibacter sacchari belonging to glycoside hydrolase family 10

open access: yesFEBS Open Bio, EarlyView.
Fervidibacter sacchari is a member of the bacterial phylum Armatimonadota, only a few of which have been cultivated. It is a hyperthermophilic polysaccharide degradation specialist. We show that a secreted enzyme, Fsa02490Xyn, is an endoxylanase belonging to glycoside hydrolase family 10.
Nicole Torosian   +10 more
wiley   +1 more source

Identification of host cytosolic sensors and bacterial factors regulating the type I interferon response to Legionella pneumophila.

open access: yesPLoS Pathogens, 2009
Legionella pneumophila is a gram-negative bacterial pathogen that replicates in host macrophages and causes a severe pneumonia called Legionnaires' Disease. The innate immune response to L. pneumophila remains poorly understood.
Kathryn M Monroe   +2 more
doaj   +1 more source

RNA-Binding Proteins Driving the Regulatory Activity of Small Non-coding RNAs in Bacteria

open access: yesFrontiers in Molecular Biosciences, 2020
Small non-coding RNAs (sRNAs) are critical post-transcriptional regulators of gene expression. Distinct RNA-binding proteins (RBPs) influence the processing, stability and activity of bacterial small RNAs.
Ana P. Quendera   +6 more
doaj   +1 more source

A Newly Discovered Bordetella Species Carries a Transcriptionally Active CRISPR-Cas with a Small Cas9 Endonuclease [PDF]

open access: yes, 2015
Background Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated genes (cas) are widely distributed among bacteria.
Dudley, Edward G.   +7 more
core   +2 more sources

Membrane vesicle-mediated release of bacterial RNA

open access: yesScientific Reports, 2015
Many Gram-negative bacterial species release outer membrane vesicles (OMVs) that interact with the host by delivering virulence factors. Here, we report for the first time that RNA is among the wide variety of bacterial components that are associated ...
Annika E. Sjöström   +3 more
semanticscholar   +1 more source

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