Results 21 to 30 of about 445,040 (271)

Structured RNA Contaminants in Bacterial Ribo-Seq

mSphere, 2020
Ribosome profiling (Ribo-Seq) is a powerful method to study translation in bacteria. However, Ribo-Seq signal can be observed across RNAs that one would not expect to be bound by ribosomes.
Brayon J. Fremin, Ami S. Bhatt
doaj   +1 more source

Structural signatures of thermal adaptation of bacterial ribosomal RNA, transfer RNA, and messenger RNA. [PDF]

PLoS ONE, 2017
Temperature adaptation of bacterial RNAs is a subject of both fundamental and practical interest because it will allow a better understanding of molecular mechanism of RNA folding with potential industrial application of functional thermophilic or ...
Clara Jegousse, Yuedong Yang, Jian Zhan, Jihua Wang, Yaoqi Zhou   +4 more
doaj   +1 more source

Evaluation of rRNA depletion methods for capturing the RNA virome from environmental surfaces

BMC Research Notes, 2023
Objective Metatranscriptomic analysis of RNA viromes on built-environment surfaces is hampered by low RNA yields and high abundance of rRNA. Therefore, we evaluated the quality of libraries, efficiency of rRNA depletion, and viral detection sensitivity ...
Yuh Shiwa, Tomoya Baba, Maria A. Sierra, JangKeun Kim, Christopher E. Mason, Haruo Suzuki   +5 more
doaj   +1 more source

Novel molecular markers for the detection of methanogens and phylogenetic analyses of methanogenic communities

Frontiers in Microbiology, 2015
Methanogenic Archaea produce approximately one billion tons of methane annually, but their biology remains largely unknown. This is partially due to the large phylogenetic and phenotypic diversity of this group of organisms, which inhabit various anoxic ...
Lukasz eDziewit, Adam ePyzik, Krzysztof eRomaniuk, Adam eSobczak, Adam eSobczak, Pawel eSzczesny, Pawel eSzczesny, Leszek eLipinski, Dariusz eBartosik, Lukasz eDrewniak   +9 more
doaj   +1 more source

Interplay between bacterial 5′-NAD-RNA decapping hydrolase NudC and DEAD-box RNA helicase CsdA in stress responses

mSystems, 2023
Both prokaryotic and eukaryotic RNAs can be 5′-capped by the metabolite nicotinamide adenine dinucleotide (NAD). Nudix hydrolases, such as bacterial NudC, specifically remove NAD-caps; however, the molecular and cellular functions of these ...
Milda Mickutė, Renatas Krasauskas, Kotryna Kvederavičiūtė, Gytė Tupikaitė, Aleksandr Osipenko, Algirdas Kaupinis, Monika Jazdauskaitė, Raminta Mineikaitė, Mindaugas Valius, Viktoras Masevičius, Giedrius Vilkaitis   +10 more
doaj   +1 more source

In planta Transcriptome Analysis of Pseudomonas syringae

Bio-Protocol, 2018
Profiling bacterial transcriptome in planta is challenging due to the low abundance of bacterial RNA in infected plant tissues. Here, we describe a protocol to profile transcriptome of a foliar bacterial pathogen, Pseudomonas syringae pv.
Tatsuya Nobori, Kenichi Tsuda
doaj   +1 more source

Targeting SARS-CoV-2 receptor-binding domain to cells expressing CD40 improves protection to infection in convalescent macaques

Nature Communications, 2021
In this study, Marlin et al. provide insights into the potential use of subunit vaccines that induce a high level of protection against SARS-CoV-2 in animal models.
Romain Marlin, Veronique Godot, Sylvain Cardinaud, Mathilde Galhaut, Severin Coleon, Sandra Zurawski, Nathalie Dereuddre-Bosquet, Mariangela Cavarelli, Anne-Sophie Gallouët, Pauline Maisonnasse, Léa Dupaty, Craig Fenwick, Thibaut Naninck, Julien Lemaitre, Mario Gomez-Pacheco, Nidhal Kahlaoui, Vanessa Contreras, Francis Relouzat, Raphaël Ho Tsong Fang, Zhiqing Wang, Jerome Ellis, Catherine Chapon, Mireille Centlivre, Aurelie Wiedemann, Christine Lacabaratz, Mathieu Surenaud, Inga Szurgot, Peter Liljeström, Delphine Planas, Timothée Bruel, Olivier Schwartz, Sylvie van der Werf, Giuseppe Pantaleo, Mélanie Prague, Rodolphe Thiébaut, Gerard Zurawski, Yves Lévy, Roger Le Grand   +37 more
doaj   +1 more source

RNA Dynamics in Aging Bacterial Spores [PDF]

Cell, 2012
Upon starvation, the bacterium Bacillus subtilis enters the process of sporulation, lasting several hours and culminating in formation of a spore, the most resilient cell type known. We show that a few days following sporulation, the RNA profile of spores is highly dynamic.
Einat Segev, Yoav Smith, Sigal Ben-Yehuda   +2 more
openaire   +3 more sources

Overlapping transcription and bacterial RNA removal [PDF]

Proceedings of the National Academy of Sciences, 2014
The precise understanding of the biology of a living cell requires the identification and quantification of the molecular components necessary to sustain life. One such element is RNA. Two independent high-throughput strategies are available to identify the entire collection of RNA molecules produced by a cell population, which is currently known as ...
Lasa Uzcudun, Íñigo, Villanueva San Martín, Maite   +1 more
openaire   +5 more sources

Microarray analysis of defined Mycobacterium tuberculosis populations using RNA amplification strategies

BMC Genomics, 2008
Background The amplification of bacterial RNA is required if complex host-pathogen interactions are to be studied where the recovery of bacterial RNA is limited.
Butcher Philip D, Senner Claire, Laing Ken, Waddell Simon J   +3 more
doaj   +1 more source