Results 21 to 30 of about 477,009 (248)
Evaluation of rRNA depletion methods for capturing the RNA virome from environmental surfaces
BMC Research Notes, 2023 Objective Metatranscriptomic analysis of RNA viromes on built-environment surfaces is hampered by low RNA yields and high abundance of rRNA. Therefore, we evaluated the quality of libraries, efficiency of rRNA depletion, and viral detection sensitivity ...
Yuh Shiwa +5 more
doaj +1 more source
mSystems, 2023 Both prokaryotic and eukaryotic RNAs can be 5′-capped by the metabolite nicotinamide adenine dinucleotide (NAD). Nudix hydrolases, such as bacterial NudC, specifically remove NAD-caps; however, the molecular and cellular functions of these ...
Milda Mickutė +10 more
doaj +1 more source
In planta Transcriptome Analysis of Pseudomonas syringae
Bio-Protocol, 2018 Profiling bacterial transcriptome in planta is challenging due to the low abundance of bacterial RNA in infected plant tissues. Here, we describe a protocol to profile transcriptome of a foliar bacterial pathogen, Pseudomonas syringae pv.
Tatsuya Nobori, Kenichi Tsuda
doaj +1 more source
Nature Communications, 2021 In this study, Marlin et al. provide insights into the potential use of subunit vaccines that induce a high level of protection against SARS-CoV-2 in animal models.
Romain Marlin +37 more
doaj +1 more source
RNA Dynamics in Aging Bacterial Spores [PDF]
Cell, 2012 Upon starvation, the bacterium Bacillus subtilis enters the process of sporulation, lasting several hours and culminating in formation of a spore, the most resilient cell type known. We show that a few days following sporulation, the RNA profile of spores is highly dynamic.
Einat Segev +2 more
openaire +3 more sources
Overlapping transcription and bacterial RNA removal [PDF]
Proceedings of the National Academy of Sciences, 2014 The precise understanding of the biology of a living cell requires the identification and quantification of the molecular components necessary to sustain life. One such element is RNA. Two independent high-throughput strategies are available to identify the entire collection of RNA molecules produced by a cell population, which is currently known as ...
Lasa Uzcudun, Íñigo +1 more
openaire +5 more sources
Bacterial small RNAs in the Genus Rickettsia [PDF]
BMC Genomics, 2015 Rickettsia species are obligate intracellular Gram-negative pathogenic bacteria and the etiologic agents of diseases such as Rocky Mountain spotted fever (RMSF), Mediterranean spotted fever, epidemic typhus, and murine typhus. Genome sequencing revealed that R. prowazekii has ~25 % non-coding DNA, the majority of which is thought to be either "junk DNA"
Casey L. C. Schroeder +8 more
openaire +3 more sources
Prediction of Novel Bacterial Small RNAs From RIL-Seq RNA–RNA Interaction Data
Frontiers in Microbiology, 2021 The genomic revolution and subsequent advances in large-scale genomic and transcriptomic technologies highlighted hidden genomic treasures. Among them stand out non-coding small RNAs (sRNAs), shown to play important roles in post-transcriptional ...
Amir Bar +3 more
doaj +1 more source
BMC Genomics, 2008 Background The amplification of bacterial RNA is required if complex host-pathogen interactions are to be studied where the recovery of bacterial RNA is limited.
Butcher Philip D +3 more
doaj +1 more source
RNA‐Seq for Bacterial Gene Expression [PDF]
Current Protocols in Nucleic Acid Chemistry, 2018 AbstractRNA sequencing (RNA‐seq) has become the preferred method for global quantification of bacterial gene expression. With the continued improvements in sequencing technology and data analysis tools, the most labor‐intensive and expensive part of an RNA‐seq experiment is the preparation of sequencing libraries, which is also essential for the ...
Poulsen, Line Dahl, Vinther, Jeppe
openaire +3 more sources