Results 31 to 40 of about 2,524,971 (285)

Comparison of DNA and RNA sequencing of total nucleic acids from human cervix for metagenomics

open access: yesScientific Reports, 2021
Although metagenomics and metatranscriptomics are commonly used to identify bacteria and viruses in human samples, few studies directly compare these strategies.
Laila Sara Arroyo Mühr   +4 more
doaj   +1 more source

Interplay between bacterial 5′-NAD-RNA decapping hydrolase NudC and DEAD-box RNA helicase CsdA in stress responses

open access: yesmSystems, 2023
Both prokaryotic and eukaryotic RNAs can be 5′-capped by the metabolite nicotinamide adenine dinucleotide (NAD). Nudix hydrolases, such as bacterial NudC, specifically remove NAD-caps; however, the molecular and cellular functions of these ...
Milda Mickutė   +10 more
doaj   +1 more source

In planta Transcriptome Analysis of Pseudomonas syringae

open access: yesBio-Protocol, 2018
Profiling bacterial transcriptome in planta is challenging due to the low abundance of bacterial RNA in infected plant tissues. Here, we describe a protocol to profile transcriptome of a foliar bacterial pathogen, Pseudomonas syringae pv.
Tatsuya Nobori, Kenichi Tsuda
doaj   +1 more source

Targeting SARS-CoV-2 receptor-binding domain to cells expressing CD40 improves protection to infection in convalescent macaques

open access: yesNature Communications, 2021
In this study, Marlin et al. provide insights into the potential use of subunit vaccines that induce a high level of protection against SARS-CoV-2 in animal models.
Romain Marlin   +37 more
doaj   +1 more source

RNA Dynamics in Aging Bacterial Spores [PDF]

open access: yesCell, 2012
Upon starvation, the bacterium Bacillus subtilis enters the process of sporulation, lasting several hours and culminating in formation of a spore, the most resilient cell type known. We show that a few days following sporulation, the RNA profile of spores is highly dynamic.
Einat Segev   +2 more
openaire   +3 more sources

Overlapping transcription and bacterial RNA removal [PDF]

open access: yesProceedings of the National Academy of Sciences, 2014
The precise understanding of the biology of a living cell requires the identification and quantification of the molecular components necessary to sustain life. One such element is RNA. Two independent high-throughput strategies are available to identify the entire collection of RNA molecules produced by a cell population, which is currently known as ...
Lasa Uzcudun, Íñigo   +1 more
openaire   +5 more sources

Bacterial ribonucleases and their roles in RNA metabolism

open access: yesCritical reviews in biochemistry and molecular biology, 2019
Ribonucleases (RNases) are mediators in most reactions of RNA metabolism. In recent years, there has been a surge of new information about RNases and the roles they play in cell physiology.
D. Bechhofer, M. Deutscher
semanticscholar   +1 more source

Microarray analysis of defined Mycobacterium tuberculosis populations using RNA amplification strategies

open access: yesBMC Genomics, 2008
Background The amplification of bacterial RNA is required if complex host-pathogen interactions are to be studied where the recovery of bacterial RNA is limited.
Butcher Philip D   +3 more
doaj   +1 more source

Extracellular vesicles deliver Mycobacterium RNA to promote host immunity and bacterial killing

open access: yesEMBO Reports, 2019
Extracellular vesicles (EVs) have been shown to carry microbial components and function in the host defense against infections. In this study, we demonstrate that Mycobacterium tuberculosis (M.tb) RNA is delivered into macrophage‐derived EVs through an M.
Yong Cheng, Jeff S. Schorey
semanticscholar   +1 more source

Bacterial small RNAs in the Genus Rickettsia [PDF]

open access: yesBMC Genomics, 2015
Rickettsia species are obligate intracellular Gram-negative pathogenic bacteria and the etiologic agents of diseases such as Rocky Mountain spotted fever (RMSF), Mediterranean spotted fever, epidemic typhus, and murine typhus. Genome sequencing revealed that R. prowazekii has ~25 % non-coding DNA, the majority of which is thought to be either "junk DNA"
Casey L. C. Schroeder   +8 more
openaire   +3 more sources

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