Results 31 to 40 of about 477,009 (248)

The bacterial enhancer-dependent RNA polymerase [PDF]

Biochemical Journal, 2016
Transcription initiation is highly regulated in bacterial cells, allowing adaptive gene regulation in response to environment cues. One class of promoter specificity factor called sigma54 enables such adaptive gene expression through its ability to lock the RNA polymerase down into a state unable to melt out promoter DNA for transcription initiation ...
Vidya C. Darbari, Vidya C. Darbari, Nan Zhang, Nan Zhang, Martin Buck, Robert Glyde, Xiaodong Zhang   +6 more
openaire   +5 more sources

Elimination of bacterial DNA during RNA isolation from sputum: Bashing bead vortexing is preferable over prolonged DNase treatment.

PLoS ONE, 2019
Sputum often contains large amounts of contaminating bacterial DNA that, if not eliminated during RNA isolation, may interfere with gene expression studies. During RNA isolation only repeated DNase treatment can effectively remove contaminating bacterial
Csilla Paska, Imre Barta, Orsolya Drozdovszky, Balazs Antus   +3 more
doaj   +1 more source

Host A-to-I RNA editing signatures in intracellular bacterial and single-strand RNA viral infections

Frontiers in Immunology, 2023
BackgroundMicrobial infection is accompanied by remodeling of the host transcriptome. Involvement of A-to-I RNA editing has been reported during viral infection but remains to be elucidated during intracellular bacterial infections.ResultsHerein we ...
Zhi-Yuan Wei, Zhi-Yuan Wei, Zhi-Yuan Wei, Zhi-Xin Wang, Zhi-Xin Wang, Zhi-Xin Wang, Jia-Huan Li, Jia-Huan Li, Jia-Huan Li, Yan-Shuo Wen, Yan-Shuo Wen, Yan-Shuo Wen, Di Gao, Di Gao, Di Gao, Shou-Yue Xia, Shou-Yue Xia, Shou-Yue Xia, Yu-Ning Li, Yu-Ning Li, Yu-Ning Li, Xu-Bin Pan, Xu-Bin Pan, Xu-Bin Pan, Yan-Shan Liu, Yun-Yun Jin, Yun-Yun Jin, Yun-Yun Jin, Jian-Huan Chen, Jian-Huan Chen, Jian-Huan Chen   +30 more
doaj   +1 more source

The Phage-Encoded N-Acetyltransferase Rac Mediates Inactivation of Pseudomonas aeruginosa Transcription by Cleavage of the RNA Polymerase Alpha Subunit

Viruses, 2020
In this study, we describe the biological function of the phage-encoded protein RNA polymerase alpha subunit cleavage protein (Rac), a predicted Gcn5-related acetyltransferase encoded by phiKMV-like viruses.
Pieter-Jan Ceyssens, Jeroen De Smet, Jeroen Wagemans, Natalia Akulenko, Evgeny Klimuk, Subray Hedge, Marleen Voet, Hanne Hendrix, Jan Paeshuyse, Bart Landuyt, Hua Xu, John Blanchard, Konstantin Severinov, Rob Lavigne   +13 more
doaj   +1 more source

Bacterial RNA Biology on a Genome Scale [PDF]

Molecular Cell, 2018
Bacteria are an exceedingly diverse group of organisms whose molecular exploration is experiencing a renaissance. While the classical view of bacterial gene expression was relatively simple, the emerging view is more complex, encompassing extensive post-transcriptional control involving riboswitches, RNA thermometers, and regulatory small RNAs (sRNAs ...
Hör, Jens, Gorski, Stanislaw A, Vogel, Jörg   +2 more
openaire   +3 more sources

An RNA Surprise in Bacterial Effector Mechanisms [PDF]

Cell Host & Microbe, 2019
Bacterial pathogens secrete effector proteins to manipulate host signaling proteins and cellular structures. In this issue of Cell Host & Microbe, Pagliuso et al. (2019) propose an effector mechanism in Listeria monocytogenes whereby an RNA-binding protein associates with bacterial RNA that stimulates RIG-I (retinoic acid inducible gene I)-based innate
Gerovac, Milan, Vogel, Jörg
openaire   +3 more sources

Small RNAs in Bacterial Virulence and Communication [PDF]

Microbiology Spectrum, 2016
ABSTRACT Bacterial pathogens must endure or adapt to different environments and stresses during transmission and infection. Posttranscriptional gene expression control by regulatory RNAs, such as small RNAs and riboswitches, is now considered central to adaptation in many bacteria, including pathogens.
Sarah L. Svensson, Cynthia M. Sharma
openaire   +2 more sources

Novel Peptide Conjugates of Modified Oligonucleotides for Inhibition of Bacterial RNase P

Frontiers in Pharmacology, 2019
Novel alternatives to traditional antibiotics are now of great demand for the successful treatment of microbial infections. Here, we present the engineering and properties of new oligonucleotide inhibitors of RNase P, an essential bacterial enzyme.
Darya Novopashina, Darya Novopashina, Mariya Vorobyeva, Anton Nazarov, Anna Davydova, Nikolay Danilin, Lyudmila Koroleva, Lyudmila Koroleva, Andrey Matveev, Alevtina Bardasheva, Nina Tikunova, Nina Tikunova, Maxim Kupryushkin, Dmitrii Pyshnyi, Dmitrii Pyshnyi, Sidney Altman, Sidney Altman, Alya Venyaminova   +17 more
doaj   +1 more source

From omics to AI—mapping the pathogenic pathways in type 2 diabetes

FEBS Letters, EarlyView.
Integrating multi‐omics data with AI‐based modelling (unsupervised and supervised machine learning) identify optimal patient clusters, informing AI‐driven accurate risk stratification. Digital twins simulate individual trajectories in real time, guiding precision medicine by matching patients to targeted therapies.
Siobhán O'Sullivan, Lu Qi, Pierre Zalloua   +2 more
wiley   +1 more source

Exploring lipid diversity and minimalism to define membrane requirements for synthetic cells

FEBS Letters, EarlyView.
Designing the lipid membrane of synthetic cells is a complex task, in which its various roles (among them solute transport, membrane protein support, and self‐replication) should all be integrated. In this review, we report the latest top‐down and bottom‐up advances and discuss compatibility and complexity issues of current engineering approaches ...
Sergiy Gan, Victoria Scarpelli, Marten Exterkate   +2 more
wiley   +1 more source