Ribozyme biotechnology: emerging opportunities for medical applications in the RNA therapeutics era [PDF]
Medical ReviewRNA-based medicines have transformed modern therapeutics, exemplified by the clinical success of small interfering RNAs (siRNAs), antisense oligonucleotides (ASOs), and mRNA vaccines.
Zhang Jingjing +7 more
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Directed Evolution of T7 RNA Polymerase Minimizes dsRNA By-product and Enables High-Fidelity mRNA Synthesis for Demanding Therapeutic Applications [PDF]
ResearchT7 RNA polymerase (T7 RNAP) is the most widely used enzyme for synthesizing therapeutic mRNA. However, RNA transcribed by T7 RNAP often contains double-stranded RNA (dsRNA) by-products that trigger innate immune responses and complicate purification ...
Weitong Qin +6 more
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RNA catalytic activity as a probe of chaperone-mediated RNA folding. [PDF]
Methods Mol Biol, 2014 For structured RNAs that possess catalytic activity, this activity provides a powerful probe for measuring the progress of folding and the effects of RNA chaperone proteins on the folding rate. The crux of this approach is that only the natively folded RNA is able to perform the catalytic reaction.
Gracia B, Russell R.
europepmc +4 more sources
Crystal structures and snapshots along Tpt1-catalyzed phosphate transfer from nucleic acid to NAD+ [PDF]
Nature CommunicationsTpt1/TRPT1/KptA family proteins are evolutionarily conserved in all three domains of life. In fungi and plants, Tpt1 transfers 2’-PO4 2- from tRNA splice junction to NAD+, which is the final step of tRNA maturation and is critical for the function of ...
Chulei Cao +17 more
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Evidence for a non-catalytic ion-binding site in multiple RNA-dependent RNA polymerases. [PDF]
PLoS ONE, 2012 A high-affinity divalent cation-binding site located proximal to the catalytic center has been identified in several RNA-dependent RNA polymerases (RdRps), but the characteristics of such a site have not been systematically studied.
Heli A M Mönttinen +2 more
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Multiplexed RNA profiling by regenerative catalysis enables blood-based subtyping of brain tumors
Nature Communications, 2023 Current technologies to subtype glioblastoma (GBM), the most lethal brain tumor, require highly invasive brain biopsies. Here, we develop a dedicated analytical platform to achieve direct and multiplexed profiling of circulating RNAs in extracellular ...
Yan Zhang +12 more
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Catalytic promiscuity in the RNA World may have aided the evolution of prebiotic metabolism.
PLoS Computational Biology, 2021 The Metabolically Coupled Replicator System (MCRS) model of early chemical evolution offers a plausible and efficient mechanism for the self-assembly and the maintenance of prebiotic RNA replicator communities, the likely predecessors of all life forms ...
Dániel Vörös +2 more
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Frontiers in Chemistry, 2022 Three protein targets from SARS-CoV-2, the viral pathogen that causes COVID-19, are studied: the main protease, the 2′-O-RNA methyltransferase, and the nucleocapsid (N) protein. For the main protease, the nucleophilicity of the catalytic cysteine C145 is
Suhasini M. Iyengar +6 more
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F1000Research, 2016 Since the discovery of the first catalytic RNA in 1981, the field of ribozyme research has developed from the discovery of catalytic RNA motifs in nature and the elucidation of their structures and catalytic mechanisms, into a field of engineering and ...
Sabine Müller +4 more
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Modulation of RNase E activity by alternative RNA binding sites.
PLoS ONE, 2014 Endoribonuclease E (RNase E) affects the composition and balance of the RNA population in Escherichia coli via degradation and processing of RNAs. In this study, we investigated the regulatory effects of an RNA binding site between amino acid residues 25
Daeyoung Kim +8 more
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