Results 1 to 10 of about 379,319 (234)

RNA catalytic activity as a probe of chaperone-mediated RNA folding. [PDF]

Methods Mol Biol, 2014
For structured RNAs that possess catalytic activity, this activity provides a powerful probe for measuring the progress of folding and the effects of RNA chaperone proteins on the folding rate. The crux of this approach is that only the natively folded RNA is able to perform the catalytic reaction.
Gracia B, Russell R.
europepmc   +5 more sources

The catalytic mechanism of the RNA methyltransferase METTL3

eLife, 2023
The complex of methyltransferase-like proteins 3 and 14 (METTL3-14) is the major enzyme that deposits N6-methyladenosine (m6A) modifications on messenger RNA (mRNA) in humans. METTL3-14 plays key roles in various biological processes through its methyltransferase (MTase) activity.
Corbeski, Ivan, Vargas-Rosales, Pablo Andrés, Bedi, Rajiv Kumar, Deng, Jiahua, Coelho, Dylan, Braud, Emmanuelle, Iannazzo, Laura, Li, Yaozong, Huang, Danzhi, Ethève-Quelquejeu, Mélanie, Cui, Qiang, Caflisch, Amedeo   +11 more
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Catalytic RNA and RNA Splicing [PDF]

American Zoologist, 1989
The capacity of Watson-Crick base-pair complementarity to direct informational transactions basic to gene expression has long been appreciated. Among RNA molecules, it mediates mRNA-tRNA codon-anticodon pairing and the 16S rRNA-mRNA Shine-Dalgarno interaction.
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Ribozymes: the characteristics and properties of catalytic RNAs [PDF]

FEMS Microbiology Reviews, 1999
Ribozymes, or catalytic RNAs, were discovered a little more than 15 years ago. They are found in the organelles of plants and lower eukaryotes, in amphibians, in prokaryotes, in bacteriophages, and in viroids and satellite viruses that infect plants. An example is also known of a ribozyme in hepatitis delta virus, a serious human pathogen.
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Catalytic RNA world relics in Dicer RNAs [PDF]

International Journal of Genetics, 2009
RNA interference (RNAi) is a naturally occurring phenomenon of RNA-mediated gene silencing that is highly conserved among multicellular organisms. In the first step of the pathway, long double- stranded RNA molecules are chopped into shorter duplexes with 2 nucleotide overhangs at both 3 ' ends by an endonuclease dubbed Dicer, the structure of which ...
Priyanka Dhar, Sayak Ganguli, Protip Basu, Abhijit Datta, Paushali Roy, Sanjay Kumar Dey   +5 more
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RNA-catalyzed evolution of catalytic RNA

Proceedings of the National Academy of Sciences
An RNA polymerase ribozyme that was obtained by directed evolution can propagate a functional RNA through repeated rounds of replication and selection, thereby enabling Darwinian evolution. Earlier versions of the polymerase did not have sufficient copying fidelity to propagate functional information, but a new variant with improved fidelity can ...
Nikolaos Papastavrou, David P. Horning, Gerald F. Joyce   +2 more
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RNA ligase ribozymes with a small catalytic core

Scientific Reports, 2023
AbstractCatalytic RNAs, or ribozymes, catalyze diverse chemical reactions that could have sustained primordial life in the hypothetical RNA world. Many natural ribozymes and laboratory evolved ribozymes exhibit efficient catalysis mediated by elaborate catalytic cores within complex tertiary structures.
Yoko Nomura, Yohei Yokobayashi
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Cross-Catalytic Replication of an RNA Ligase Ribozyme [PDF]

Chemistry & Biology, 2004
A self-replicating RNA ligase ribozyme was converted to a cross-catalytic format whereby two ribozymes direct each other's synthesis from a total of four component substrates. Each ribozyme binds two RNA substrates and catalyzes their ligation to form the opposing ribozyme.
Gerald F. Joyce, Dong-Eun Kim
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Catalytic Role for RNA in DNA Replication [PDF]

Nature New Biology, 1972
THERE are two biochemical systems which probably evolved before the development of accurate polynucleotide-specified protein synthesis: these are the system for polynucleotide replication and the machinery of protein synthesis itself1, 2. Before accurately specified proteins became available, these processes were perhaps catalysed by polynucleotide ...
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Optimal Random Libraries for the Isolation of Catalytic RNA [PDF]

RNA Biology, 2005
The relationship between ribozyme size and catalytic activity is of fundamental importance for RNA catalysis and molecular evolution in the RNA world. We have performed a series of competitive in vitro selection experiments to probe the relationship using RNA libraries containing size-heterogeneous random regions.
Coleman, Tricia M., Huang, Faqing
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