Results 121 to 130 of about 249,749 (166)
Some of the next articles are maybe not open access.
Transition State Stabilization by a Catalytic RNA
Science, 2002The hairpin ribozyme catalyzes sequence-specific cleavage of RNA through transesterification of the scissile phosphate. Vanadate has previously been used as a transition state mimic of protein enzymes that catalyze the same reaction. Comparison of the 2.2 angstrom resolution structure of a vanadate–hairpin ribozyme complex with structures of precursor ...
Peter B, Rupert +3 more
openaire +2 more sources
The Catalytic Mechanism of DNA and RNA Polymerases
ACS Catalysis, 2018DNA and RNA polymerases (Pols) catalyze nucleic acid biosynthesis in all domains of life, with implications for human diseases and health. Pols carry out nucleic acid extension through the addition of one incoming nucleotide trisphosphate at the 3′-OH terminus of the growing primer strand, at every catalytic cycle. Thus, Pol catalysis involves chemical
Vito Genna, Elisa Donati, Marco De Vivo
openaire +1 more source
Structure‐Selective Catalytic Alkylation of DNA and RNA
Angewandte Chemie, 2012A variety of nucleic acids can be catalytically alkylated with rhodium-carbenoids generated from diazo compounds in aqueous buffer through an NH insertion process (see scheme; MES=2-(N-morpholino)ethanesulfonic acid). The method specifically targets unpaired bases such as those present in single strands, turn regions, and overhangs while leaving double-
Tishinov, Kiril +3 more
openaire +4 more sources
The efficiency and versatility of catalytic RNA: implications for an RNA world
Gene, 1993Because RNA is chemically and structurally dissimilar from protein, the finding of catalytic activity in RNA was initially surprising. Quantitative measurements of reaction rates show that RNA can be as efficient a catalyst as protein. On the other hand, the potential versatility of RNA to catalyze diverse types of reactions has only begun to be ...
openaire +2 more sources
The FASEB Journal, 1993
We describe the structures and catalytic properties of several naturally occurring self-cleaving RNA motifs that give 2', 3' cyclic phosphate products. The hammerhead and hairpin motifs are derived from plant pathogenic RNAs and the delta motif is part of the human hepatitis delta element. A fourth motif from Neurospora is less well characterized.
D M, Long, O C, Uhlenbeck
openaire +2 more sources
We describe the structures and catalytic properties of several naturally occurring self-cleaving RNA motifs that give 2', 3' cyclic phosphate products. The hammerhead and hairpin motifs are derived from plant pathogenic RNAs and the delta motif is part of the human hepatitis delta element. A fourth motif from Neurospora is less well characterized.
D M, Long, O C, Uhlenbeck
openaire +2 more sources
Antisense Catalytic RNAs as Therapeutic Agents
1994Publisher Summary This chapter presents an overview of well-characterized ribozymes with an emphasis on the problems that remain to be overcome before ribozyme therapy can be made a useful form of medical treatment. The chapter illustrates that many techniques are currently being used to enhance ribozyme activity.
D, Castanotto, J J, Rossi, N, Sarver
openaire +2 more sources
Amplification, mutation and selection of catalytic RNA
Gene, 1989RNA, by virtue of its genotypic and phenotypic properties, is a suitable substrate for molecular evolution in the laboratory. We have developed techniques for the rapid amplification, mutation and selection of catalytic RNA. By combining these techniques in an iterative fashion, we are attempting to construct an RNA-based evolving system. Such a system
openaire +2 more sources
In Vitro Selection of Catalytic RNA
1996After the first examples of catalytic RNAs raised to prominence earlier proposals that RNA was a likely protagonist in the dawn of evolution, molecular biologists began to ask in earnest about the range of possible RNA activities. This question provided much of the impetus for the development of in vitro RNA selection (Ellington and Szostak 1990; Green
K. P. Williams, D. P. Bartel
openaire +1 more source
Recent insights on RNA folding mechanisms from catalytic RNA
Cellular and Molecular Life Sciences (CMLS), 2000Methods for probing RNA structure in real time have revealed that initial folding steps are complete in less than a second. Refolding of large catalytic RNAs in vitro often results in long-lived intermediates that reach the native structure very slowly.
openaire +2 more sources