Results 101 to 110 of about 407,557 (297)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Homochiral growth through enantiomeric cross-inhibition

, 2004
The stability and conservation properties of a recently proposed polymerization model are studied. The achiral (racemic) solution is linearly unstable once the relevant control parameter (here the fidelity of the catalyst) exceeds a critical value.
A. Brandenburg, A. C. Andersen, C. Woese, D. K. Kondepudi, D. K. Kondepudi, D. K. Kondepudi, D. K. Kondepudi, F. Frank, F. H. C. Crick, G. F. Joyce, H. Haken, J. A. D. Wattis, J. A. D. Wattis, J. Bailey, J. L. Bada, J. L. Bada, K. E. Nelson, L. E. Orgel, M. Nilsson, M. Pooga, N. V. Pogodina, P. E. Hare, P. E. Nielsen, P. G. H. Sandars, R. A. Hegstrom, R. Kippenhahn, S. Höfner, S. Rasmussen, T. Tedeschi, V. A. Avetisov, V. I. Goldanskii, W. A. Bonner, Y. Saito   +32 more
core   +4 more sources

Large Phenotypic Enhancement of Structured Random RNA Pools. [PDF]

, 2020
Laboratory evolution of functional RNAs has applications in many areas of chemical and synthetic biology. In vitro selections critically depend on the presence of functional molecules, such as aptamers and ribozymes, in the starting sequence pools.
Chizzolini, Fabio, Dingilian, Armine I, Lupták, Andrej, Oumais, Mona, Passalacqua, Luiz FM, Szostak, Jack W   +5 more
core  

Ketogenic Diet as an Epigenetic Therapy in SETD1B‐Related Epilepsy

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Histone lysine methyltransferases such as SETD1B regulate chromatin structure and gene transcription. Ketone bodies, including butyrate, act as histone deacetylase inhibitors. We report a 4‐year‐old boy with SETD1B‐related absence epilepsy, refractory to conventional medications, who achieved sustained > 90% seizure reduction on the Modified ...
Erica Tsang, Brian S. Gloss, Jessica P. Hayes, Andrew J. A. Holland, Manoj P. Menezes, Joceline A. Branson, Shekeeb S. Mohammad, Jingya J. Yan, Shrujna Patel, Velda X. Han, Russell C. Dale   +10 more
wiley   +1 more source

Reconciling Ligase Ribozyme Activity with Fatty Acid Vesicle Stability

Life, 2014
The “RNA world” and the “Lipid world” theories for the origin of cellular life are often considered incompatible due to the differences in the environmental conditions at which they can emerge.
Fabrizio Anella, Christophe Danelon
doaj   +1 more source

ALDOA Promotes Glycolysis and NLRP3/GSDMD Pyroptosis to Accelerate ALS Progression

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Amyotrophic lateral sclerosis (ALS) is characterized by progressive motor neuron degeneration. Glycolytic dysregulation is implicated in disease progression, yet the underlying mechanisms remain unclear. This study investigates how Aldolase A (ALDOA) drives ALS progression through glycolysis‐mediated motor neuron pyroptosis.
Kaixin Yan, Yan Jiang, Yuxuan Yong, Tianshuo Zhang, Niannian Zhang, Qianqian Zeng, Xue Gong, Li Meng, Fangfang Bi, Yongmin Liu   +9 more
wiley   +1 more source

Specific catalytically impaired DDX3X mutants form sexually dimorphic hollow condensates

Nature Communications
Mutations in the RNA helicase DDX3X, implicated in various cancers and neurodevelopmental disorders, often impair RNA unwinding and translation. However, the mechanisms underlying the impairment and the differential interactions of DDX3X mutants with ...
Michael C. Owens, Hui Shen, Amber Yanas, Maria Saraí Mendoza-Figueroa, Ellen Lavorando, Xiaoyu Wei, Him Shweta, Hsin-Yao Tang, Yale E. Goldman, Kathy Fange Liu   +9 more
doaj   +1 more source

The catalytic mechanism of the RNA methyltransferase METTL3

eLife
The complex of methyltransferase-like proteins 3 and 14 (METTL3-14) is the major enzyme that deposits N6-methyladenosine (m6A) modifications on messenger RNA (mRNA) in humans.
Ivan Corbeski, Pablo Andrés Vargas-Rosales, Rajiv Kumar Bedi, Jiahua Deng, Dylan Coelho, Emmanuelle Braud, Laura Iannazzo, Yaozong Li, Danzhi Huang, Mélanie Ethève-Quelquejeu, Qiang Cui, Amedeo Caflisch   +11 more
doaj   +1 more source