Results 251 to 260 of about 423,061 (280)

Double-Stranded Viral RNA

1969
Publisher Summary The pace of research on RNA virus replication and the huge amount of related literature that has accumulated in the past 6 years attest to the wide interest in the topic. There has also been some controversy and apparent confusion, probably largely because of pressure for publication priority. In this chapter, methods for detecting,
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Infectious double-stranded poliovirus RNA

Virology, 1964
Abstract Evidence is presented for the existence of an infectious double-stranded poliovirus RNA in HeLa cells which is relatively resistant to RNase digestion, has a buoyant density of 1.58 g cm −3 in Cs 2 SO 4 , a sedimentation coefficient of 16–20 S in sucrose gradients, and elutes from methylated albumin columns at 0.84 M NaCl.
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Cellular response to double‐stranded RNA

Journal of Cellular Biochemistry, 1991
AbstractThe study of double‐stranded RNA (dsRNA) encompasses a variety of fields. Basic research in this area has contributed to a greater mechanistic understanding of gene induction, tumor cell growth arrest, the establishment of antiviral states, and immunomodulation.
D S, Haines, K I, Strauss, D H, Gillespie   +2 more
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Herpes-virus and double-stranded RNA

Experientia, 1973
On a cherche a determiner la presence d'un ARN bi-cat\'enaire lors de la r\'eplication du virus herp\'etique. La pr\'ecipitation de LiCl 2M, la filtration sur gel Sephadex, la chromatographie sur cellulose, la s\'edimentation en gradient de densit\'e et l'induction d'interf\'eron in vivo et in vitro semblent d\'emontrer qu'une telle structure n'est pas
R, Perez-Bercoff, G, Carrara, A, Dolei, G, Rita   +3 more
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Double-stranded RNA of Ibaraki virus

Virology, 1977
Abstract The Ibaraki virus RNA is double-stranded. The base composition of the RNA is 27.7% A, 27.1% U, 23.0% G, and 22.2% C. Ibaraki virus is classified as belonging to the orbivirus genus of the family Reoviridae.
Y, Suzuki, Y, Saito, S, Nakagawa
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Double-stranded tobacco mosaic virus RNA

Virology, 1969
Abstract Isolation of the double-stranded viral RNA in fractionated cell-free extracts from radioactive tobacco mosaic virus-infected tobacco leaves suggests that the double-stranded tobacco mosaic virus RNA is associated with a cytoplasmic structure or organelle and not with nuclei.
R K, Ralph, S J, Wojcik
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Direct Cloning of Double-Stranded RNAs

2015
Most annotated genomes show a large number of sense-antisense transcripts that can generate double-stranded RNAs. We describe a method to clone these dsRNAs from total RNA preparations.
Manli, Shen, Marina, Falaleeva, Natalia, Korotkova, Stefan, Stamm   +3 more
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RNA interference and double-stranded-RNA-activated pathways

Biochemical Society Transactions, 2004
RNAi (RNA interference) has become a powerful tool to determine gene function. Different methods of expressing the short ds (double-stranded) RNA intermediates required for interference in mammalian systems have been developed, including the introduction of si (short interfering) RNAs by direct transfection or driven from transfected plasmids or ...
C A, Sledz, B R G, Williams
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Gene silencing by double-stranded RNA

Current Opinion in Cell Biology, 2001
Eukaryotes silence gene expression in the presence of double-stranded RNA homologous to the silenced gene. Silencing occurs by the targeted degradation of mRNA. Biochemical reactions that recapitulate this phenomenon generate RNA fragments of 21--23 nucleotides from the double-stranded RNA.
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Double-Stranded RNAs as Gene Activators

1994
In 1963 Gomatos and Tamm discovered the first natural dsRNA, namely the genomic RNA of reoviruses. A few years later, Lampson et al. (1967) established that dsRNA is the active agent in an extract of a Penicillium funiculosum preparation which, following injection into rabbits, induces interferon (IFN) in serum. They also showed that various natural (i.
S, Landolfo, M, Gariglio, G, Gribaudo, G, Garotta   +3 more
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