Results 81 to 90 of about 502,163 (208)
Alternative approach to generate shRNA from cDNA
BioTechniques, 2005 Short hairpin RNA (shRNA) synthesized from vector-based expression is as effective as short interfering RNA (siRNA) synthesized in vitro for suppressing the expression of their corresponding genes.
Anh Dinh, Yin-Yuan Mo
doaj +1 more source
Viruses, 2020 Generation of recombinant negative-stranded RNA viruses (NSVs) from plasmids involves in vivo reconstitution of biologically active nucleocapsids and faces a unique antisense problem where the negative-sense viral genomic RNAs can hybridize to viral ...
Xiaonan Ma, Zhenghe Li
doaj +1 more source
Ribonuclease III mechanisms of double-stranded RNA cleavage
Wiley Interdisciplinary Reviews - RNA, 2013 Double‐stranded(ds) RNA has diverse roles in gene expression and regulation, host defense, and genome surveillance in bacterial and eukaryotic cells.
A. Nicholson
semanticscholar +1 more source
Double-stranded RNA elements associated with the MVX disease of Agaricus bisporus [PDF]
, 2003 Double-stranded RNA (dsRNA) has been isolated from Agaricus bisporus fruit bodies exhibiting a wide range of disease symptoms. The symptoms which occurred singularly or in combination included; bare cropping areas on commercial beds (primordia disruption)
Adie, Bruce A. T. +4 more
core +1 more source
The rice diacylglycerol kinase family: functional analysis using transient RNA interference
Frontiers in Plant Science, 2012 Diacylglycerol kinase (DGK) is a pivotal enzyme that phosphorylates diacylglycerol (DAG) to form phosphatidic acid (PA). The production of PA from phospholipase D (PLD) and the coupled phospholipase C (PLC)/DGK route is an important signaling process in ...
Hongliang eGe +6 more
doaj +1 more source
The double-stranded RNA binding domain of human Dicer functions as a nuclear localization signal
RNA: A publication of the RNA Society, 2013 In mammalian cells, the cytoplasmic function of Dicer is well established, but evidence for its roles in the nucleus is fragmentary. This work demonstrates that the C-terminal double-stranded RNA binding domain of human Dicer (hDicer) includes a nuclear ...
Michael Doyle +7 more
semanticscholar +1 more source
Can we always sweep the details of RNA-processing under the carpet? [PDF]
, 2013 RNA molecules follow a succession of enzyme-mediated processing steps from transcription until maturation. The participating enzymes, for example the spliceosome for mRNAs and Drosha and Dicer for microRNAs, are also produced in the cell and their copy-numbers fluctuate over time.
arxiv +1 more source
Adenosine to inosine editing by ADAR2 requires formation of a ternary complex on the GluR-B R/G site [PDF]
, 2002 RNA editing by members of the ADAR (adenosine deaminase that acts on RNA) enzyme family involves hydrolytic deamination of adenosine to inosine within the context of a double-stranded pre-mRNA substrate.
Collins, Cynthia H. +2 more
core +1 more source
Biology Direct, 2007 Background It is generally believed that life first evolved from single-stranded RNA (ssRNA) that both stored genetic information and catalyzed the reactions required for self-replication. Presentation of the hypothesis By modeling early genome evolution
de Roos Albert DG
doaj +1 more source
Combinatorics Of RNA Structures With Pseudoknots [PDF]
arXiv, 2007 In this paper we derive the generating function of RNA structures with pseudoknots. We enumerate all $k$-noncrossing RNA pseudoknot structures categorized by their maximal sets of mutually intersecting arcs. In addition we enumerate pseudoknot structures over circular RNA. For 3-noncrossing RNA structures and RNA secondary structures we present a novel
arxiv