Results 151 to 160 of about 34,514 (185)
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Nucleic Acid Detection through RNA‐Guided Protease Activity in Type III‐E CRISPR‐Cas Systems
ChemBioChem, 2023AbstractRNA‐guided protease activity was recently discovered in the type III‐E CRISPR‐Cas systems (Craspase), providing a novel platform for engineering a protein probe instead of the commonly used nucleic acid probe in nucleic acid detection assays.
Qiuqiu He +8 more
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Get ready for the CRISPR/Cas system: A beginner's guide to the engineering and design of guide RNAs
The Journal of Gene Medicine, 2021AbstractThe clustered regularly interspaced short palindromic repeats (CRISPR) system is a state‐of‐the‐art tool for versatile genome editing that has advanced basic research dramatically, with great potential for clinic applications. The system consists of two key molecules: a CRISPR‐associated (Cas) effector nuclease and a single guide RNA.
Shufeng Ma +4 more
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Transposon-encoded CRISPR–Cas systems direct RNA-guided DNA integration
Nature, 2019Conventional CRISPR-Cas systems maintain genomic integrity by leveraging guide RNAs for the nuclease-dependent degradation of mobile genetic elements, including plasmids and viruses. Here we describe a notable inversion of this paradigm, in which bacterial Tn7-like transposons have co-opted nuclease-deficient CRISPR-Cas systems to catalyse RNA-guided ...
Sanne E, Klompe +3 more
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Advances in guide RNA design for editing plant genomes using CRISPR-Cas systems
2021This chapter discusses the general rules for selecting target sites for genome editing using the CRISPR-Cas technology and summarizes the bioinformatic tools that can be used to design sgRNA sequences.
Kaiyuan Chen +4 more
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2022
Type I is the most prevalent CRISPR system found in nature. It can be further defined into six subtypes, from I-A to I-G. Among them, the Type I-A CRISPR-Cas systems are almost exclusively found in hyperthermophilic archaeal organisms. The system achieves RNA-guided DNA degradation through the concerted action of a CRISPR RNA containing complex Cascade
Chunyi, Hu, Ailong, Ke
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Type I is the most prevalent CRISPR system found in nature. It can be further defined into six subtypes, from I-A to I-G. Among them, the Type I-A CRISPR-Cas systems are almost exclusively found in hyperthermophilic archaeal organisms. The system achieves RNA-guided DNA degradation through the concerted action of a CRISPR RNA containing complex Cascade
Chunyi, Hu, Ailong, Ke
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CRISPR‐Cas systems and RNA‐guided interference
WIREs RNA, 2013AbstractClustered regularly interspaced short palindromic repeats (CRISPR) together with associated sequences (cas) form the CRISPR‐Cas system, which provides adaptive immunity against viruses and plasmids in bacteria and archaea. Immunity is built through acquisition of short stretches of invasive nucleic acids into CRISPR loci as ‘spacers'.
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Functions and Applications of RNA-Guided CRISPR-Cas Immune Systems
2014Clustered regularly interspaced short palindromic repeats (CRISPRs), together with CRISPR-associated sequences (cas) constitute the CRISPR-Cas adaptive immune system in bacteria and archaea. Adaptive immunity is built into CRISPR arrays through the uptake of small pieces of invasive nucleic acids, such as viruses and plasmids.
Rodolphe Barrangou, Philippe Horvath
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