Results 1 to 10 of about 586,860 (123)
Evaluation of reference genes for gene expression analysis in Japanese flounder (Paralichthys olivaceus) under temperature stress [PDF]
BMC GenomicsBackground Quantitative Real-time PCR (qRT-PCR) is a powerful technique to analyze gene expression patterns by measuring the relative abundance of mRNA transcription levels.
Ping Han +4 more
doaj +2 more sources
Selection of reference genes for expression analysis using quantitative real-time PCR in the pea aphid, Acyrthosiphon pisum (Harris) (Hemiptera, Aphidiae). [PDF]
PLoS ONE, 2014 To facilitate gene expression study and obtain accurate qRT-PCR analysis, normalization relative to stable expressed housekeeping genes is required. In this study, expression profiles of 11 candidate reference genes, including actin (Actin), elongation ...
Chunxiao Yang +3 more
doaj +1 more source
The final step of 40S ribosomal subunit maturation is controlled by a dual key lock
eLife, 2021 Preventing premature interaction of pre-ribosomes with the translation apparatus is essential for translational accuracy. Hence, the final maturation step releasing functional 40S ribosomal subunits, namely processing of the 18S ribosomal RNA 3′ end, is ...
Laura Plassart +12 more
doaj +1 more source
Polysomally Protected Viruses [PDF]
Phys. Biol. 18 046009 (2021), 2021 It is conceivable that an RNA virus could use a polysome, that is, a string of ribosomes covering the RNA strand, to protect the genetic material from degradation inside a host cell. This paper discusses how such a virus might operate, and how its presence might be detected by ribosome profiling.
arxiv +1 more source
BMC Molecular and Cell Biology, 2020 Background We have previously reported 18S and 25S ribosomal RNA molecules in Candida albicans resistant to processive 5′ → 3′ exonuclease, appearing as cells approached stationary growth phase. Initial analysis pointed to extra phosphate(s) at their 5′-
Jacob Fleischmann +4 more
doaj +1 more source
Transcriptional repressor NIR functions in the ribosome RNA processing of both 40S and 60S subunits. [PDF]
PLoS ONE, 2012 BACKGROUND: NIR was identified as an inhibitor of histone acetyltransferase and it represses transcriptional activation of p53. NIR is predominantly localized in the nucleolus and known as Noc2p, which is involved in the maturation of the 60S ribosomal ...
Jianguo Wu +7 more
doaj +1 more source
Cells, 2021 Cockayne syndrome (CS) is a developmental disorder with symptoms that are typical for the aging body, including subcutaneous fat loss, alopecia, and cataracts.
Mingyue Qiang +5 more
doaj +1 more source
Growth-laws and invariants from ribosome biogenesis in lower Eukarya [PDF]
Phys. Rev. Research 3, 013020 (2021), 2020 Eukarya and Bacteria are the most evolutionarily distant domains of life, which is reflected by differences in their cellular structure and physiology. For example, Eukarya feature membrane-bound organelles such as nuclei and mitochondria, whereas Bacteria have none.
arxiv +1 more source
Stably expressed housekeeping genes across developmental stages in the two-spotted spider mite, Tetranychus urticae. [PDF]
PLoS ONE, 2015 Quantitative real-time PCR (qRT-PCR) is a reliable and reproducible technique for measuring mRNA expression. To facilitate gene expression studies and obtain more accurate qRT-PCR analysis, normalization relative to stable housekeeping genes is mandatory.
Chunxiao Yang +3 more
doaj +1 more source
RNA Biology, 2022 snR30/U17 is a highly conserved H/ACA RNA that is required for maturation of the small ribosomal subunit in eukaryotes. By base-pairing to the expansion segment 6 (ES6) of 18S ribosomal RNA (rRNA), the snR30 H/ACA Ribonucleoprotein (RNP) indirectly ...
Timothy J. Vos, Ute Kothe
doaj +1 more source