Results 91 to 100 of about 37,099 (261)

Reference loci for RT-qPCR analysis of differentiating human embryonic stem cells [PDF]

, 2013
Background: Selecting stably expressed reference genes is essential for proper reverse transcription quantitative polymerase chain reaction gene expression analysis. However, this choice is not always straightforward. In the case of differentiating human
De Sutter, Petra, Deforce, Dieter, Heindryckx, Björn, O'Leary, Thomas, Van Neste, Christophe, Vandesompele, Jo, Vossaert, Liesbeth   +6 more
core   +2 more sources

Sekuen Penyandi 18S Ribosomal RNA dan Ubiquitin pada Pandanus sp. Asal Riau (Sequences Encoding 18S Ribosomal RNA and Ubiquitin on Pandanus sp. from Riau)

JURNAL BIOS LOGOS, 2018
Abstrak Gen penyandi 18S ribosomal RNA (18S rRNA) dan ubiquitin merupakan anggota dari housekeeping genes yang sering digunakan sebagai kontrol internal pada analisis ekspresi.  Penelitian bertujuan mengisolasi kedua gen tersebut pada tanaman Pandanus sp asal Riau.
Roslim, Dewi Indriyani, Nuryani, Nurin, Herman, Herman   +2 more
openaire   +3 more sources

HIV‐1 establishes immediate latency in T cells expressing the viral Nef protein

FEBS Open Bio, EarlyView.
Nef is a viral protein often omitted from HIV‐1 reporter viruses. Consequently, its role in viral latency is unclear. We developed three novel dual reporter HIV‐1 derivatives that express Nef and allow for detection of latent and productive infection. Using these reporters, we show that Nef does not affect the establishment of immediate viral latency ...
Cindy Lam, Ivan Sadowski
wiley   +1 more source

In Situ Detection of Aspergillus 18S Ribosomal RNA in Invasive Pulmonary Apergillosis.

Internal Medicine, 1999
We attempted to evaluate the usefulness of in situ hybridization (ISH) in the specific diagnosis of Aspergillus pulmonary infection.We used an ISH technique using a multiple digoxigenin-incorporating probe, which was constructed by means of the polymerase chain reaction (PCR) from the 18S ribosomal RNA of Aspergillus fumigatus.We studied twelve ...
M, Kobayashi, H, Sonobe, T, Ikezoe, E, Hakoda, Y, Ohtsuki, H, Taguchi   +5 more
openaire   +3 more sources

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

FEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada, Mina Iwaki, Kyosuke Hagiri, Rei Izumi, Masahiko Harata, Chihiro Horigome   +5 more
wiley   +1 more source

Mycosphaerella podagrariae - a necrotrophic phytopathogen forming a special cellular interaction with its host Aegopodium podagraria [PDF]

, 2010
We present a new kind of cellular interaction found between Mycosphaerella podagrariae and Aegopodium podagraria, which is remarkably different to the interaction type of the obligate biotrophic fungus Cymadothea trifolii, another member of the ...
Bauer, R., Crous, P.W., Groenewald, J.Z., Simon, U.K., Stierhof, Y.D.   +4 more
core   +3 more sources

Developmental, Neuroanatomical and Cellular Expression of Genes Causing Dystonia

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Dystonia is one of the most common movement disorders, with variants in multiple genes identified as causative. However, an understanding of which developmental stages, brain regions, and cell types are most relevant is crucial for developing relevant disease models and therapeutics.
Darren Cameron, Nicholas E. Clifton, Daniel Cabezas de la Fuente, Peter Holmans, Nicholas J. Bray, Kathryn J. Peall   +5 more
wiley   +1 more source

Molecular Characterization of a Non–Babesia divergens Organism Causing Zoonotic Babesiosis in Europe

Emerging Infectious Diseases, 2003
In Europe, most reported human cases of babesiosis have been attributed, without strong molecular evidence, to infection with the bovine parasite Babesia divergens.
Barbara L. Herwaldt, Simone Cacciò, Filippo Gherlinzoni, Horst Aspöck, Susan B. Slemenda, PierPaolo Piccaluga, Giovanni Martinelli, Renate Edelhofer, Ursula Hollenstein, Giovanni Poletti, Silvio Pampiglione, Karin Löschenberger, Sante Tura, Norman J. Pieniazek   +13 more
doaj   +1 more source

RNomics: a computational search for box C/D snoRNA genes in the D. melanogaster genome. [PDF]

, 2004
Motivation: In eukaryotes, the family of non-coding RNA genes includes a number of genes encoding small nucleolar RNAs (mainly C/D and H/ACA snoRNAs), which act as guides in the maturation or post-transcriptional modifications of target RNA molecules ...
ACCARDO MC, CALOGERO RA, DIGILIO FA, FURIA, MARIA, GIORDANO, ENNIO, IAZZETTI, GIOVANNI, RICCARDO S   +6 more
core  

Unraveling the Molecular Mechanisms of Glioma Recurrence: A Study Integrating Single‐Cell and Spatial Transcriptomics

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Glioma recurrence severely impacts patient prognosis, with current treatments showing limited efficacy. Traditional methods struggle to analyze recurrence mechanisms due to challenges in assessing tumor heterogeneity, spatial dynamics, and gene networks.
Lei Qiu, Yinjiao Fei, Jiaxuan Ding, Kexin Shi, Jinyan Luo, Yuchen Zhu, Xingjian Sun, Gefei Jiang, Yuandong Cao, Weilin Xu, Shu Zhou   +10 more
wiley   +1 more source