Purification of human transcription factor IIIC and its binding to the gene for ribosomal 5S RNA.
Nucleic Acids Research, 1989 Transcription factor hTFIIIC was purified from cytoplasmic extracts of HeLa cells using four different chromatographic steps. This procedure yields a protein fraction which actively supports transcription in reconstitution assays and contains five major ...
H. R. Schneider +3 more
semanticscholar +1 more source
Purification of 70S Ribosomes from Bacillus subtilis
Bio-Protocol, 2015 The eubacterial ribosome (70S) is a macromolecular complex that is composed of a small (30S) subunit and a large (50S) subunit. The small subunit comprises the 16S ribosomal RNA (rRNA) and more than 20 ribosomal proteins (r-proteins), whereas the large ...
Shota Suzuki +2 more
doaj +1 more source
Two distinct conformations of rat liver ribosomal 5S RNA.
Nucleic Acids Research, 1982 Three different conformers of rat liver 5S ribosomal RNA were investigated by partial nuclease cleavage technique using S1 nuclease and cobra venom endoribonuclease (CVE) as conformational probes.
I. Toots +5 more
semanticscholar +1 more source
60S ribosome biogenesis requires rotation of the 5S ribonucleoprotein particle [PDF]
, 2014 During eukaryotic ribosome biogenesis, nascent ribosomal RNA (rRNA) forms pre-ribosomal particles containing ribosomal proteins and assembly factors. Subsequently, these immature rRNAs are processed and remodelled.
Bange, Gert +8 more
core +1 more source
Mitochondrial DNA. Part B. Resources, 2019 The complete chloroplast genome of Juniperus squamata, a shrubby conifer of ornamental value, is determined in this study. The complete chloroplast genome size is 127,792 bp in length.
Siyu Xie +4 more
doaj +1 more source
In vitro Assessment of RNA Polymerase I Activity
Bio-Protocol, 2017 In eukaryotic cells transcriptional processes are carried out by three different RNA polymerases: RNA polymerase I which specifically transcribes ribosomal RNA (rRNA), RNA polymerase II which transcribes protein-coding genes to yield messenger RNAs ...
Marzia Govoni
doaj +1 more source
Kinetoplastid Specific RNA-Protein Interactions in Trypanosoma cruzi Ribosome Biogenesis. [PDF]
PLoS ONE, 2015 RNA binding proteins (RBP) play essential roles in the highly conserved and coordinated process of ribosome biogenesis. Our laboratory has previously characterized two essential and abundant RBPs, P34 and P37, in Trypanosoma brucei which are required for
Khan Umaer, Noreen Williams
doaj +1 more source
Essential Assembly Factor Rpf2 Forms Novel Interactions within the 5S RNP in
mSphere, 2017 Ribosome biogenesis is a highly complex and conserved cellular process that is responsible for making ribosomes. During this process, there are several assembly steps that function as regulators to ensure proper ribosome formation.
Anyango D. Kamina +3 more
doaj +1 more source
Identification of Sex and Female's Reproductive Stage in Commercial Fish Species through the Quantification of Ribosomal Transcripts in Gonads. [PDF]
PLoS ONE, 2016 The estimation of maturity and sex of fish stocks in European waters is a requirement of the EU Data Collection Framework as part of the policy to improve fisheries management.
Iratxe Rojo-Bartolomé +3 more
doaj +1 more source
Control of ribosome traffic by position-dependent choice of synonymous codons [PDF]
Phys. Biol. 10 (2013) 056011, 2013 Messenger RNA encodes a sequence of amino acids by using codons. For most amino acids there are multiple synonymous codons that can encode the amino acid. The translation speed can vary from one codon to another, thus there is room for changing the ribosome speed while keeping the amino acid sequence and hence the resulting protein.
arxiv +1 more source