Results 121 to 130 of about 498,663 (149)

Small Nuclear RNAs and RNA Processing

1983
I. Summary Ribosomal RNA, transfer RNA, and messenger RNAs, which comprise about 99% of the cellular RNA, are part of the protein-synthesizing machinery. Many studies in the last 15 years have established the presence of another class of RNA, “small nuclear RNAs” (snRNAs) that account for 0.1-1% of the total cellular RNA.
Harris Busch, Ram Reddy
openaire   +3 more sources

Small Nuclear RNA U2 Is Base-Paired to Heterogeneous Nuclear RNA

Science, 1982
Eukaryotic cells contain a set of low molecular weight nuclear RNA's. One of the more abundant of these is termed U2 RNA. The possibility that U2 RNA is hydrogen-bonded to complementary sequences in other nuclear RNA's was investigated. Cultured human (HeLa) cells were treated with a psoralen derivative that cross-links RNA chains that are base-paired ...
Thoru Pederson, James P. Calvet, Leslie M. Meyer   +2 more
openaire   +3 more sources

Subnuclear particles containing a small nuclear RNA and heterogeneous nuclear RNA

Journal of Molecular Biology, 1981
Abstract Five of the stable low molecular weight RNA species in the HeLa cell nucleus have been localized in RNP complexes in the cell nucleus. The two abundant species C and D and the three minor species F, G′ and H are found in RNP particles following two different methods of preparation.
Gary Zieve, Sheldon Penman
openaire   +3 more sources

The status of small nuclear RNA in the ribonucleoprotein fibrils containing heterogeneous nuclear RNA

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1981
hnRNP are made of two classes of unit, monoparticles and heterogeneous complexes. The monoparticles are much more easily dissociated by salt than the heterogeneous complexes. We made use of this differential salt sensitivity to determine the localization of snRNA in hnRNP.
Monique Jacob, Hélène Gallinaro
openaire   +2 more sources

The role of small nuclear RNAs in RNA splicing

Current Opinion in Cell Biology, 1993
Recent genetic and biochemical experiments have revealed an intimate and dynamic role for small nuclear RNAs (snRNAs) in multiple steps of RNA-splicing reactions. Both snRNA-substrate and snRNA-snRNA interactions are involved. These interactions concern not only splice site and branch point definition, but also the catalytic reactions of the first and ...
openaire   +3 more sources

Small nuclear RNAs in messenger RNA and ribosomal RNA processing.

The FASEB Journal, 1993
Ribonucleoproteins (RNPs) play essential roles in many aspects of gene expression. Two families of nuclear RNPs are involved in the processing of primary transcripts made by RNA polymerases I and II (pol I and II), two of the three polymerases present in the nuclei of eukaryotic cells.
Iain W. Mattaj, David Tollervey, Bertrand Séraphin   +2 more
openaire   +2 more sources

Genes for human U4 small nuclear RNA

Gene, 1986
A study of human genes coding for U4 small nuclear RNA is presented. It is known from previous studies that mammalian cells contain three major U4 RNA species, designated U4A, U4B, and U4C (Krol and Branlant, 1981). A clone was isolated from a human DNA library which contained two transcriptionally active genes for U4 RNA.
Polly Weller, Christina Bark, Jan Zabielski, Ulf Pettersson   +3 more
openaire   +3 more sources

Biosynthesis of small nuclear RNAs in human cells

Journal of Cellular Physiology, 1983
AbstractWe have examined some aspects of the biosynthesis of human small nuclear RNAs (snRNAs). The sensitivity of U5 and U4 snRNA synthesis to α‐amanitin in whole cells suggests that RNA polymerase II is involved in the synthesis of these RNA species, in addition to that of U1, U2, and U3 snRNA.
George L. Eliceiri, Settara C. Chandrasekharappa, James H. Smith   +2 more
openaire   +3 more sources

Associations of small nuclear RNAs in human cells

Journal of Cellular Physiology, 1983
AbstractSeveral observations have been made about the associations of small nuclear RNAs (snRNAs) in human cells. When nuclear RNA was extracted with phenol and chloroform under standard nondenaturing conditions, the proportion of the nuclear snRNA content that cosedimented with high molecular weight RNA was very low.
George L. Eliceiri, James H. Smith
openaire   +3 more sources

Isolation of Small Nuclear RNAs

1994
For isolation of small nuclear (sn)RNAs from maize leaf tissue, we use a slightly modified procedure described originally by Kiss et al. (1985) for dicot plants. This procedure consists of two basic steps: (1) purification of maize nuclei and (2) extraction of snRNAs.
Tamás Kiss, Witold Filipowicz
openaire   +2 more sources