Results 151 to 160 of about 2,786,263 (356)

Activation of influenza virus RNA polymerase by the 5' and 3' terminal duplex of genomic RNA

, 2003
The current model for influenza virus mRNA transcription involves the sequential interaction of the viral polymerase with the 5'- and 3'-ends of vRNA, with each RNA-protein interaction triggering a polymerase function necessary for cap-primed ...
Tiley, Laurence, Klumpp, Klaus, Digard, Paul, Lee, Michael T   +3 more
core  

Recombination in enteroviruses is a biphasic replicative process involving the generation of greater-than genome length 'imprecise' intermediates [PDF]

, 2014
Recombination in enteroviruses provides an evolutionary mechanism for acquiring extensive regions of novel sequence, is suggested to have a role in genotype diversity and is known to have been key to the emergence of novel neuropathogenic variants of ...
David J Evans, Woodman, Andrew, Andrew Woodman, Kym Lowry (222718), Jonathan Cook (3435386), Andrew Woodman (577477), Cook, Jonathan, Cook, Jonathan P., David J. Evans (208308), Lowry, Kym, David J. Evans, Evans, David J., Kym Lowry, Jonathan Cook   +13 more
core   +1 more source

Structure-guided chemical modification of guide RNA enables potent non-viral in vivo genome editing

Nature Biotechnology, 2017
Efficient genome editing with Cas9–sgRNA in vivo has required the use of viral delivery systems, which have limitations for clinical applications. Translational efforts to develop other RNA therapeutics have shown that judicious chemical modification of ...
Hao Yin, Chun‐Qing Song, Sneha Suresh, Qiongqiong Wu, Stephen Walsh, Luke H. Rhym, Esther Mintzer, M. Bolukbasi, L. Zhu, Kevin J. Kauffman, Haiwei Mou, Alicia Oberholzer, Junmei Ding, Suet‐Yan Kwan, R. Bogorad, T. Zatsepin, V. Koteliansky, S. Wolfe, Wen Xue, R. Langer, Daniel G. Anderson   +20 more
semanticscholar   +1 more source

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source

Positive-sense RNA viruses reveal the complexity and dynamics of the cellular and viral epitranscriptomes during infection

Nucleic Acids Research, 2018
More than 140 post-transcriptional modifications (PTMs) are known to decorate cellular RNAs, but their incidence, identity and significance in viral RNA are still largely unknown.
W. McIntyre, Rachel Netzband, G. Bonenfant, Jason M. Biegel, Clare M. Miller, G. Fuchs, E. Henderson, Manoj Arra, M. Canki, D. Fabris, Cara T. Pager   +10 more
semanticscholar   +1 more source

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

FEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner, Hainan Zhu, Carol Christine Bosholm, Heuy‐Ching Wang, Tracy Criswell, Anthony Atala, Jian‐Xing Ma, Yuanyuan Zhang   +7 more
wiley   +1 more source

RNA viral vectors for improved Agrobacterium-mediated transient expression of heterologous proteins in Nicotiana benthamiana cell suspensions and hairy roots

BMC Biotechnology, 2012
BackgroundPlant cell suspensions and hairy root cultures represent scalable protein expression platforms. Low protein product titers have thus far limited the application of transient protein expression in these hosts.
Jeffrey S. Larsen, W. Curtis
semanticscholar   +1 more source

Pharmacological inhibition of the PERK pathway modulates hepatocellular carcinoma growth and immune signaling

FEBS Open Bio, EarlyView.
Pharmacological inhibition of PERK in a DEN‐induced mouse model of liver cancer does not reduce tumor burden but alters cellular stress signaling. Despite blocking PERK activity, downstream stress responses, including CHOP expression, remain active, suggesting compensatory mechanisms within the unfolded protein response that may influence tumor ...
Ada Lerma‐Clavero, Maria Kopsida, Nathalie Arendt, Hans Lennernäs, Markus Sjöblom, Femke Heindryckx   +5 more
wiley   +1 more source

dsRNA-Seq: Identification of Viral Infection by Purifying and Sequencing dsRNA

Viruses, 2019
RNA viruses are a major source of emerging and re-emerging infectious diseases around the world. We developed a method to identify RNA viruses that is based on the fact that RNA viruses produce double-stranded RNA (dsRNA) while replicating. Purifying and
Carolyn J. Decker, Halley R. Steiner, Laura L. Hoon-Hanks, James H. Morrison, Kelsey C. Haist, Alex C. Stabell, Eric M. Poeschla, Thomas E. Morrison, Mark D. Stenglein, Sara L. Sawyer, Roy Parker   +10 more
doaj   +1 more source

Cyclic azapeptide CD36 ligand attenuates cardiac injury and reduces long‐chain fatty acid accumulation after myocardial ischemia–reperfusion in mice

FEBS Open Bio, EarlyView.
In a murine model of myocardial ischemia and reperfusion (MI/R), the CD36 azapeptide ligand MPE‐298 reduces cardiac injury and transiently lowers left ventricular long‐chain fatty acids (LCFAs) accumulation 3 h after reperfusion, accompanied by a decrease of oxidative stress and inflammation‐associated genes' expression in the heart and adipose tissue.
Jade Gauvin, Naghme Radmannia, David N. Huynh, Liliane Ménard, Caroline Daneault, Maïté Veilleux, Ahsanullah Ahsanullah, André C. Carpentier, William D. Lubell, Matthieu Ruiz, Huy Ong, Sylvie Marleau, Simon‐Pierre Gravel   +12 more
wiley   +1 more source