CRISPR-Guided Proximity Labeling of RNA-Protein Interactions. [PDF]
Genes (Basel), 2022 Proximity labeling employs modified biotin ligases or peroxidases that produce reactive radicals to covalently label proximate proteins with biotin in living cells. The resulting biotinylated proteins can then be isolated and identified. A combination of
Lu M, Wang Z, Wang Y, Ren B.
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The Plethora of RNA-Protein Interactions Model a Basis for RNA Therapies. [PDF]
Genes (Basel)The notion of RNA-based therapeutics has gained wide attractions in both academic and commercial institutions. RNA is a polymer of nucleic acids that has been proven to be impressively versatile, dating to its hypothesized RNA World origins, evidenced by
Dansereau SJ +3 more
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Computational tools to study RNA-protein complexes
Frontiers in Molecular Biosciences, 2022 RNA is the key player in many cellular processes such as signal transduction, replication, transport, cell division, transcription, and translation. These diverse functions are accomplished through interactions of RNA with proteins.
Sneha Bheemireddy +5 more
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Novel endoribonucleases as central players in various pathways of eukaryotic RNA metabolism [PDF]
, 2010 For a long time it has been assumed that the decay of RNA in eukaryotes is mainly carried out by exoribonucleases, which is in contrast to bacteria, where endoribonucleases are well documented to initiate RNA degradation.
Tomecki, Rafał, Dziembowski, Andrzej
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RNA Proximity Labeling: A New Detection Tool for RNA–Protein Interactions
Molecules, 2021 Multiple cellular functions are controlled by the interaction of RNAs and proteins. Together with the RNAs they control, RNA interacting proteins form RNA protein complexes, which are considered to serve as the true regulatory units for post ...
Ronja Weissinger +4 more
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Decoding the role of the RNA binding activity of p53 [PDF]
, 2022 p53 is a key tumour suppressor, mutated in over half of cancers. p53’s ability to form extensive protein interactions, along with its function as a sequence-specific transcription factor, are well documented due to their roles in canonical p53 tumour ...
Moore, Samantha Louise
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Essential Assembly Factor Rpf2 Forms Novel Interactions within the 5S RNP in
mSphere, 2017 Ribosome biogenesis is a highly complex and conserved cellular process that is responsible for making ribosomes. During this process, there are several assembly steps that function as regulators to ensure proper ribosome formation.
Anyango D. Kamina +3 more
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An improved method for surface immobilisation of RNA: application to small Non-Coding RNA - mRNA pairing [PDF]
, 2013 Characterisation of RNA and its intermolecular interactions is increasing in importance as the inventory of known RNA functions continues to expand. RNA-RNA interactions are central to post-transcriptional gene regulation mechanisms in bacteria, and the ...
Darren M. Gowers (485354) +39 more
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Trim25 is an RNA-specific activator of Lin28a/TuT4-mediated uridylation [PDF]
, 2014 RNA binding proteins have thousands of cellular RNA targets and often exhibit opposite or passive molecular functions. Lin28a is a conserved RNA binding protein involved in pluripotency and tumorigenesis that was previously shown to trigger TuT4-mediated
Roy Choudhury, Nila +11 more
core +1 more source
Crystal structure of Caulobacter crescentus polynucleotide phosphorylase reveals a mechanism of RNA substrate channelling and RNA degradosome assembly [PDF]
Open Biology, 2012 Polynucleotide phosphorylase (PNPase) is an exoribonuclease that cleaves single-stranded RNA substrates with 3′–5′ directionality and processive behaviour.
Steven W. Hardwick +4 more
doaj +1 more source