Results 81 to 90 of about 1,877,035 (363)

Protein solubility and folding enhancement by interaction with RNA. [PDF]

open access: yesPLoS ONE, 2008
While basic mechanisms of several major molecular chaperones are well understood, this machinery has been known to be involved in folding of only limited number of proteins inside the cells. Here, we report a chaperone type of protein folding facilitated
Seong Il Choi   +12 more
doaj   +1 more source

RNA-Binding Proteins in Plant Immunity [PDF]

open access: yesJournal of Pathogens, 2011
Plant defence responses against pathogen infection are crucial to plant survival. The high degree of regulation of plant immunity occurs both transcriptionally and posttranscriptionally. Once transcribed, target gene RNA must be processed prior to translation. This includes polyadenylation,5′capping, editing, splicing, and mRNA export.
Woloshen, Virginia   +2 more
openaire   +2 more sources

Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer

open access: yesFEBS Letters, EarlyView.
Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani   +4 more
wiley   +1 more source

Ebola virus VP35 induces high-level production of recombinant TPL-2–ABIN-2–NF-κB1 p105 complex in co-transfected HEK-293 cells [PDF]

open access: yes, 2013
Activation of PKR (double-stranded-RNA-dependent protein kinase) by DNA plasmids decreases translation, and limits the amount of recombinant protein produced by transiently transfected HEK (human embryonic kidney)-293 cells.
Apostolaki   +47 more
core   +1 more source

RPI-Bind: a structure-based method for accurate identification of RNA-protein binding sites

open access: yesScientific Reports, 2017
RNA and protein interactions play crucial roles in multiple biological processes, while these interactions are significantly influenced by the structures and sequences of protein and RNA molecules.
Jiesi Luo   +4 more
doaj   +1 more source

Protein-mediated miRNA detection and siRNA enrichment using p19

open access: yesBioTechniques, 2010
p19 RNA binding protein from the Carnation Italian ringspot virus (CIRV) is an RNA-silencing suppressor that binds small interfering RNA (siRNA) with high affinity.
Jingmin Jin   +3 more
doaj   +1 more source

Iron enhances the binding rates and translational efficiency of iron responsive elements (IREs) mRNA with initiation factor eIF4F.

open access: yesPLoS ONE, 2021
Interaction of iron responsive elements (IRE) mRNA with the translational machinery is an early step critical in the initiation of protein synthesis.
Mateen A Khan, Artem V Domashevskiy
doaj   +1 more source

RNA-binding protein nucleolin in disease [PDF]

open access: yesRNA Biology, 2012
Nucleolin is a multifunctional protein localized primarily in the nucleolus, but also found in the nucleoplasm, cytoplasm and cell membrane. It is involved in several aspects of DNA metabolism, and participates extensively in RNA regulatory mechanisms, including transcription, ribosome assembly, mRNA stability and translation, and microRNA processing ...
Kotb, Abdelmohsen, Myriam, Gorospe
openaire   +2 more sources

Disordered but rhythmic—the role of intrinsic protein disorder in eukaryotic circadian timing

open access: yesFEBS Letters, EarlyView.
Unstructured domains known as intrinsically disordered regions (IDRs) are present in nearly every part of the eukaryotic core circadian oscillator. IDRs enable many diverse inter‐ and intramolecular interactions that support clock function. IDR conformations are highly tunable by post‐translational modifications and environmental conditions, which ...
Emery T. Usher, Jacqueline F. Pelham
wiley   +1 more source

Northwestern Blot of Protein-RNA Interaction from Young Rice Panicles

open access: yesBio-Protocol, 2013
The northwestern assay is employed to study the interaction between protein and RNA. The RNA binding proteins tend to bind to different kinds of RNA through either known domains or unknown sequences of proteins.
Saminathan Thangasamy, Guang-Yuh Jauh
doaj   +1 more source

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