Results 91 to 100 of about 2,765,005 (400)

Effects of RNA structure and salt concentration on the affinity and kinetics of interactions between pentatricopeptide repeat proteins and their RNA ligands.

PLoS ONE, 2018
Pentatricopeptide repeat (PPR) proteins are helical repeat proteins that bind specific RNA sequences via modular 1-repeat:1-nucleotide interactions. Binding specificity is dictated, in part, by hydrogen bonds between the amino acids at two positions in ...
James J McDermott, Bryce Civic, Alice Barkan   +2 more
doaj   +1 more source

Identification of Protein-RNA Interactions in Mouse Testis Tissue Using fRIP

Bio-Protocol, 2022
During development, cells must quickly switch from one cell state to the next to execute precise and timely differentiation. One method to ensure fast transitions in cell states is by controlling gene expression at the post-transcriptional level through ...
Alexis Bailey, Pedro Batista, Howard Chang, Margaret Fuller   +3 more
doaj   +1 more source

P‐glycoprotein modulates the fluidity gradient of the plasma membrane of multidrug resistant CHO cells

FEBS Letters, EarlyView.
To explore the impact of the overexpression of the multidrug‐transporter P‐glycoprotein (ABCB1) on membrane fluidity, we compared the transversal gradient of mobility and microviscosity in plasma membranes of drug‐sensitive Chinese hamster ovary cells (AuxB1) and their multidrug‐resistant derivatives (B30) using the fluorescent n‐(9‐anthroyloxy) fatty ...
Roger Busche, John R. Riordan, Burkhard Tümmler   +2 more
wiley   +1 more source

Targeted Genetic Screen in Amyotrophic Lateral Sclerosis Reveals Novel Genetic Variants with Synergistic Effect on Clinical Phenotype [PDF]

, 2017
Amyotrophic lateral sclerosis (ALS) is underpinned by an oligogenic rare variant architecture. Identified genetic variants of ALS include RNA-binding proteins containing prion-like domains (PrLDs). We hypothesized that screening genes encoding additional
Adrian Higginbottom, Adriano Chio, Ahmad Al Kheifat, Alberto Garcia Redondo, Aleksey Shatunov, Alfredo Iacoangeli, Ammar Al-Chalabi, Annelot Dekker, Bas Middelkoop, Chris Shaw, Christopher J. McDermott, Gijs Tazelaar, Guillaume M. Hautbergue, Henry Robins, Ian Blair, Isabell Niedermoser, Jan Veldink, Janine Kirby, Jesus Mora Pardina, John Landers, Johnathan Cooper-Knock, Joke van Vugt, Jonathan Glass, Jonathan Mill, Karen Morrison, Kevin Kenna, Kristelvan Eijk, Leonard van den Berg, Maarten Kooyman, Mamede de Carvalho, Marc Gotkine, Markus Weber, Matthew Kiernan, Matthew Wyles, Mattieu Moisse, Mayana Ztaz, Mbombe Kazoka, Michael van Es, Miguel Mitne Neto, Nazli Basak, Nicola Ticozzi, Orla Hardiman, Pamela J. Shaw, Paul G. Ince, Paul R. Heath, Peter Andersen, Philip van Damme, Philippe Corcia, Philippe Couratier, Project MinE ALS Sequencing Consortium, Rick van der Spek, Russel McLaughlin, Sara Pulit, Stephen Newhouse, Susana Pinto, Theresa Walsh, Vincenzo Silani, Vivian Drory, William Sproviero, Wim Robberecht, Winston Hide, Wouter van Rheenen   +61 more
core   +5 more sources

The Role of RNA Binding Proteins for Local mRNA Translation: Implications in Neurological Disorders

Frontiers in Molecular Biosciences, 2020
As neurons are one of the most highly polarized cells in our body, they require sophisticated cellular mechanisms to maintain protein homeostasis in their subcellular compartments such as axons and dendrites.
M. P. Thelen, M. Kye
semanticscholar   +1 more source

RNA binding strategies of ribosomal proteins [PDF]

Nucleic Acids Research, 1999
Structures of a number of ribosomal proteins have now been determined by crystallography and NMR, though the complete structure of a ribosomal protein-rRNA complex has yet to be solved. However, some ribosomal protein structures show strong similarity to well-known families of DNA or RNA binding proteins for which structures in complex with cognate ...
David E. Draper, Luis P. Reynaldo
openaire   +3 more sources

The characterization of RNA-binding proteins and RNA metabolism-related proteins in fungal extracellular vesicles

Frontiers in Cellular and Infection Microbiology, 2023
RNA-binding proteins (RBPs) are essential for regulating RNA metabolism, stability, and translation within cells. Recent studies have shown that RBPs are not restricted to intracellular functions and can be found in extracellular vesicles (EVs) in ...
Marianna Dallastella, Willian Klassen de Oliveira, Marcio L. Rodrigues, Marcio L. Rodrigues, Samuel Goldenberg, Lysangela R. Alves, Lysangela R. Alves   +6 more
doaj   +1 more source

Evolutionary interplay between viruses and R‐loops

FEBS Letters, EarlyView.
Viruses interact with specialized nucleic acid structures called R‐loops to influence host transcription, epigenetic states, latency, and immune evasion. This Perspective examines the roles of R‐loops in viral replication, integration, and silencing, and how viruses co‐opt or avoid these structures.
Zsolt Karányi, Zita Képes, Zoltán Szabó, Eszter Csoma, Lóránt Székvölgyi   +4 more
wiley   +1 more source

The Crystal Structure of the NHL Domain in Complex with RNA Reveals the Molecular Basis of Drosophila Brain-Tumor-Mediated Gene Regulation

Cell Reports, 2015
TRIM-NHL proteins are conserved among metazoans and control cell fate decisions in various stem cell linages. The Drosophila TRIM-NHL protein Brain tumor (Brat) directs differentiation of neuronal stem cells by suppressing self-renewal factors.
Inga Loedige, Leonhard Jakob, Thomas Treiber, Debashish Ray, Mathias Stotz, Nora Treiber, Janosch Hennig, Kate B. Cook, Quaid Morris, Timothy R. Hughes, Julia C. Engelmann, Michael P. Krahn, Gunter Meister   +12 more
doaj   +1 more source

Purification tags markedly affect self‐aggregation of CPEB3

FEBS Letters, EarlyView.
Although recombinant proteins are used to study protein aggregation in vitro, uncleaved tags can interfere with accurate interpretation. Our findings demonstrate that His₆‐GFP and His₁₂ tags significantly affect liquid droplet and amyloid fibril formation in the intrinsically disordered region (IDR) of mouse cytoplasmic polyadenylation element‐binding ...
Harunobu Saito, Yujin Lee, Motoharu Ueno, Naotaka Sekiyama, Masatomo So, Ayako Furukawa, Kenji Sugase   +6 more
wiley   +1 more source