SARS-CoV-2 and miRNA-like inhibition power [PDF]
arXiv, 2020 (1) Background: RNA viruses and especially coronaviruses could act inside host cells not only by building their own proteins, but also by perturbing the cell metabolism. We show the possibility of miRNA-like inhibitions by the SARS-CoV-2 concerning for example the hemoglobin and type I interferons syntheses, hence highly perturbing oxygen distribution ...
arxiv
RNA polymerase motors on DNA track: effects of traffic congestion on RNA synthesis [PDF]
arXiv, 2007 RNA polymerase (RNAP) is an enzyme that synthesizes a messenger RNA (mRNA) strand which is complementary to a single-stranded DNA template. From the perspective of physicists, an RNAP is a molecular motor that utilizes chemical energy input to move along the track formed by a ssDNA.
arxiv
RNA polymerase motor on DNA track: effects of interactions, external force and torque [PDF]
arXiv, 2008 RNA polymerase (RNAP) is a mobile molecular workshop that polymerizes a RNA molecule by adding monomeric subunits one by one, while moving step by step on the DNA template itself. Here we develop a theoretical model by incorporating the steric interactions of the RNAPs and their mechanochemical cycles which explicitly captures the cyclical shape ...
arxiv
Elongation-Competent Pauses Govern the Fidelity of a Viral RNA-Dependent RNA Polymerase
Cell Reports, 2015 RNA viruses have specific mutation rates that balance the conflicting needs of an evolutionary response to host antiviral defenses and avoidance of the error catastrophe.
David Dulin +7 more
doaj +1 more source
Towards the Development of a Minigenome Assay for Species A Rotaviruses
VirusesRNA virus polymerases carry out multiple functions necessary for successful genome replication and transcription. A key tool for molecular studies of viral RNA-dependent RNA polymerases (RdRps) is a ‘minigenome’ or ‘minireplicon’ assay, in which viral ...
Ola Diebold +6 more
doaj +1 more source
First Report on Detection and Molecular Characterization of Astroviruses in Mongooses
VirusesApplying a pan-astrovirus (AstV) RT-hemi-nested PCR assay, we report here high detection rates (28.3%, 15/53) of AstVs in the small Indian mongoose (Urva auropunctata) on the Caribbean Island of St. Kitts.
Jessica L. Kulberg +3 more
doaj +1 more source
Mixed molecular motor traffic on nucleic acid tracks: models of transcriptional interference and regulation of gene expression [PDF]
arXiv, 2015 RNA polymerase (RNAP) is molecular machine that polymerizes a RNA molecule, a linear heteropolymer, using a single stranded DNA (ssDNA) as the corresponding template; the sequence of monomers of the RNA is dictated by that of monomers on the ssDNA template.
arxiv
Emergent kinetics of in vitro transcription from interactions of T7 RNA polymerase and DNA [PDF]
arXivThe in vitro transcription reaction (IVT) is of growing importance for the manufacture of RNA vaccines and therapeutics. While the kinetics of the microscopic steps of this reaction (promoter binding, initiation, and elongation) are well studied, the rate law of overall RNA synthesis that emerges from this system is unclear.
arxiv
Transcribing RNA polymerases: Dynamics of Twin Supercoiled Domains [PDF]
Gene transcription by a RNA Polymerase (RNAP) enzyme requires that double-stranded DNA be locally and transiently opened, which results in an increase of DNA supercoiling downstream of the RNAP and a decrease of supercoiling upstream of it. When the DNA is initially torsionally relaxed and the RNAP experiences sufficiently large rotational drag, these ...
arxiv +1 more source
Molnupiravir; an effective drug in treating COVID-19? [PDF]
Journal of Preventive Epidemiology, 2022 Alireza Pouramini +4 more
doaj +1 more source