Results 71 to 80 of about 583,250 (311)

Identifying Different Mutation Sites Leading to Resistance to the Direct-Acting Antiviral (DAA) Sofosbuvir in Hepatitis C Virus Patients from Egypt

Microorganisms, 2022
The hepatitis C virus (HCV) is a major global health challenge and a leading cause of morbidity and mortality. Many direct-acting antivirals (DAAs) target essential macromolecules involved in the virus’ life cycle.
Aly Atef Shoun, Rania Abozahra, Kholoud Baraka, Mai Mehrez, Sarah M. Abdelhamid   +4 more
doaj   +1 more source

Interstitial Contacts in an RNA-Dependent RNA Polymerase Lattice [PDF]

Journal of Molecular Biology, 2011
Catalytic activities can be facilitated by ordered enzymatic arrays that co-localize and orient enzymes and their substrates. The purified RNA-dependent RNA polymerase from poliovirus self-assembles to form two-dimensional lattices, possibly facilitating the assembly of viral RNA replication complexes on the cytoplasmic face of intracellular membranes.
Andres B, Tellez, Jing, Wang, Elizabeth J, Tanner, Jeannie F, Spagnolo, Karla, Kirkegaard, Esther, Bullitt   +5 more
openaire   +2 more sources

Modelling stem cell differentiation related processes—A practical overview for biologists

FEBS Letters, EarlyView.
Stem cell differentiation is complex and difficult to control experimentally. This review introduces suitable computational modelling approaches that can support stem cell research, from mechanistic ODE and abstract models to multiscale and deep learning methods.
Ricco Zeegelaar, Xinqi Yan, Lucas F. Jansen Klomp, Stefano Schivo, Janine N. Post   +4 more
wiley   +1 more source

RNA elements directing in vivo assembly of the 7SK/MePCE/Larp7 transcriptional regulatory snRNP [PDF]

, 2013
Through controlling the nuclear level of active positive transcription elongation factor b (P-TEFb), the 7SK small nuclear RNA (snRNA) functions as a key regulator of RNA polymerase II transcription.
Kiss, T., Muniz, Lisa, Muniz, L., Egloff, Sylvain, Kiss, Tamás   +4 more
core   +1 more source

Antiviral Effects of a Transgenic RNA-Dependent RNA Polymerase [PDF]

Journal of Virology, 2011
ABSTRACTTransgenic expression of the RNA-dependent RNA polymerase 3Dpolinhibited infection of Theiler's murine encephalitis virus (TMEV), a picornavirus from which it was derived. Here, we infected 3Dpoltransgenic mice with another picornavirus, as well as an alphaherpesvirus and a rhabdovirus.
Jason, Kerkvliet, Louisa, Papke, Moses, Rodriguez   +2 more
openaire   +2 more sources

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Definition of the minimal viral components required for the initiation of unprimed RNA synthesis by influenza virus RNA polymerase [PDF]

, 2002
The first 11 nt at the 5' end of influenza virus genomic RNA were shown to be both necessary and sufficient for specific binding by the influenza virus polymerase.
Tiley, Laurence, Digard, Paul, Medcalf, Liz, Bishop, Konrad, Elton, Debra, Lee, Michael T   +5 more
core  

Trim25 is an RNA-specific activator of Lin28a/TuT4-mediated uridylation [PDF]

, 2014
RNA binding proteins have thousands of cellular RNA targets and often exhibit opposite or passive molecular functions. Lin28a is a conserved RNA binding protein involved in pluripotency and tumorigenesis that was previously shown to trigger TuT4-mediated
Nowak, Jakub S., Roy Choudhury, Nila, Rappsilber, Juri, Spoel, Steven H, Nila Roy Choudhury, Nowak, Jakub S, Zuo, Juan, Michlewski, Gracjan, Juan Zuo, Juri Rappsilber, Choudhury, Nila Roy, Steven H. Spoel, Gracjan Michlewski, Jakub S. Nowak, Spoel, Steven H.   +14 more
core   +1 more source

RNA-Dependent RNA Polymerase Activity in Influenza Virions [PDF]

Proceedings of the National Academy of Sciences, 1971
An RNA-dependent RNA polymerase activity has been detected in purified preparations of influenza virus. In contrast to the replicase activity induced in influenza-infected cells, the virion-associated enzyme has an absolute requirement for Mn ++ .
E, Penhoet, H, Miller, M, Doyle, S, Blatti   +3 more
openaire   +2 more sources

AscF in the mycobacterial CIII–CIV supercomplex lacks metal and nucleotide binding but links malate oxidation to respiration

FEBS Letters, EarlyView.
The mycobacterial CIII‐CIV respiratory supercomplex is an obligate assembly, encompassing several subunits of unknown functions. We have characterized the intracellular subunit AscF, and show that it is unlikely to be a sensor for metals or nucleotides, but is required for growth on nonfermentable energy sources, and likely works as an adapter for ...
Eni Rile, Riccardo Diamanti, Daniel Lundin, Soni Sharma, Robin Lissner, Coenraad P. Kuil, Martin Högbom, Dirk Bald, Dan Sjöstrand   +8 more
wiley   +1 more source