Results 71 to 80 of about 987,281 (377)

Biochemical characterization of the fidelity of poliovirus RNA-dependent RNA polymerase

Virology Journal, 2007
Background Putative high mutation rates of RNA viruses are believed to mediate undesirable phenomena, such as emergence of drug resistance. However, very little is known about biochemical fidelity rates for viral RNA-dependent RNA polymerases.
Vaccaro Joseph A, Freistadt Marion S, Eberle Karen E   +2 more
doaj   +1 more source

NeoRdRp2 with improved seed data, annotations, and scoring

Frontiers in Virology
RNA-dependent RNA polymerase (RdRp) is a marker gene for RNA viruses; thus, it is widely used to identify RNA viruses from metatranscriptome data. However, because of the high diversity of RdRp domains, it remains difficult to identify RNA viruses using ...
Shoichi Sakaguchi, Takashi Nakano, So Nakagawa, So Nakagawa, So Nakagawa   +4 more
doaj   +1 more source

Functional lability of RNA-dependent RNA polymerases in animals

PLOS Genetics, 2018
AbstractRNA interference (RNAi) requires RNA-dependent RNA polymerases (RdRPs) in many eukaryotes, and RNAi amplification constitutes the only known function for eukaryotic RdRPs. Yet in animals, classical model organisms can elicit RNAi without possessing RdRPs, and only nematode RNAi was shown to require RdRPs.
Pinzón, Natalia, Bertrand, Stéphanie, Subirana, Lucie, Busseau, Isabelle, Escriva, Hector, Seitz, Hervé   +5 more
openaire   +7 more sources

Mechanistic insights into the transcriptional arrest in the presence of Double Strand Breaks [PDF]

, 2014
Double-strand breaks (DSBs) occur frequently in the genome during genome replication or by DNA damaging agents. DNA lesions affect fundamental DNA-dependent nuclear processes, such as replication and transcription.
Bonhomme, Celine, Chen, David, Eick, Dirk, Furst, Audrey, Pankotai, Tibor, Soutoglou, Evi, Sumara, Isabella   +6 more
core   +2 more sources

Functional Importance of the Hydrophobic Residue 362 in Influenza A PB1 Subunit

Viruses, 2023
PB1, acting as the catalytic subunit of the influenza polymerase, has numerous sequentially and structurally conserved regions. It has been observed that the slight modification of residues in PB1 would greatly affect the polymerase activity and even ...
Johnson Jor-Shing Chan, Yun-Sang Tang, Chun-Yeung Lo, Pang-Chui Shaw   +3 more
doaj   +1 more source

Space constrained homology modelling: The paradigm of the RNA-dependent RNA polymerase of dengue (Type II) virus [PDF]

, 2013
Protein structure is more conserved than sequence in nature. In this direction we developed a novel methodology that significantly improves conventional homology modelling when sequence identity is low, by taking into consideration 3D structural features
Kontopoulos, DG, Kossida, S, Vlachakis, D   +2 more
core   +2 more sources

Nucleotide sequence and genomic organization of an ophiovirus associated with lettuce big-vein disease [PDF]

, 2002
The complete nucleotide sequence of an ophiovirus associated with lettuce big-vein disease has been elucidated. The genome consisted of four RNA molecules of approximately 7ò8, 1ò7, 1ò5 and 1ò4 kb.
Dullemans, A.M., Heuvel, J.F.J.M., van den, Verbeek, M., Wilk, F., van der   +3 more
core   +2 more sources

A Magnetic Tweezers Study of a RNA-Dependant RNA Polymerase [PDF]

Biophysical Journal, 2012
RNA-dependent RNA Polymerases (RdRPs) are involved in the transcription and the replication of RNA genome of many viruses. The RdRP of the bacteriophage Φ6 (P2) is located inside the viral capsid where it transcribes double-stranded RNA to yield single-stranded RNA that infects the host cytosol.Here, we have studied the transcription activity of the P2
Minna M. Poranen, Martin Depken, David Dulin, Igor D. Vilfan, Susanne Hage, Wiecher Kamping, Dennis H. Bamford, Nynke H. Dekker   +7 more
openaire   +2 more sources

A stepwise emergence of evolution in the RNA world

FEBS Letters, EarlyView.
How did biological evolution emerge from chemical reactions? This perspective proposes a gradual scenario of self‐organization among RNA molecules, where catalytic feedback on random mixtures plays the central role. Short oligomers cross‐ligate, and self‐assembly enables heritable variations. An event of template‐externalization marks the transition to
Philippe Nghe
wiley   +1 more source

Multiple effects of silymarin on the hepatitis C virus lifecycle [PDF]

, 2010
Silymarin, an extract from milk thistle (Silybum marianum), and its purified flavonolignans have been recently shown to inhibit hepatitis C virus (HCV) infection, both in vitro and in vivo.
Bourne, Nigel, Brimacombe, Claire L, Cao, Feng, Graf, Tyler N, Grove, Joe, Kane, Olivia J, Lohmann, Volker, Martinez, Laura E, McKeating, Jane A, Nahmias, Yaakov, Negash, Amina, Oberlies, Nicholas H, Owen, David M, Polyak, Stephen J, Pécheur, Eve-Isabelle, Tavis, John E, Wagoner, Jessica   +16 more
core   +1 more source