Results 61 to 70 of about 3,624,287 (199)

A Single ssRNA Segment Encoding RdRp Is Sufficient for Replication, Infection, and Transmission of Ourmia-Like Virus in Fungi

Frontiers in Microbiology, 2020
Recently, an increasing number of ourmia-like viruses have been found in fungi; however, the features of these viruses remain unknown. Here, we report a novel ourmia-like virus isolated from Sclerotinia sclerotiorum. This virus, named S.
Qihua Wang, Qihua Wang, Fan Mu, Fan Mu, Jiatao Xie, Jiatao Xie, Jiasen Cheng, Jiasen Cheng, Yanping Fu, Daohong Jiang, Daohong Jiang   +10 more
doaj   +1 more source

Impact of Remdesivir Incorporation Along the Primer Strand on SARS-CoV-2 RNA-dependent RNA polymerase [PDF]

, 2022
Remdesivir was the first antiviral drug that received emergency use authorization from the United States Food and Drug Administration and is now formally approved to treat COVID-19.
Sehr, Naseem-Khan, Madison B., Berger, Emmett M., Leddin, G. Andrés, Cisneros, Yazdan , Maghsoud   +4 more
core   +2 more sources

Insight Into the Interaction Between RNA Polymerase and VPg for Murine Norovirus Replication

Frontiers in Microbiology, 2018
Norovirus (NoV) is a leading cause of epidemic acute non-bacterial gastroenteritis, and replicates through virion protein genome-linked (VPg)-primed or de novo RNA synthesis by RNA-dependent RNA polymerase (RdRp).
Ji-Hye Lee, Beom Seok Park, Intekhab Alam, Kang R. Han, Scott B. Biering, Soo J. Kim, Jayoung Choi, Jong H. Seok, Mi S. Chung, Ho M. Kim, Seungmin Hwang, Seungmin Hwang, Kyung H. Kim   +12 more
doaj   +1 more source

RNAi in the Rhizarian Phytopathogen Plasmodiophora brassicae: The Causal Agent of Clubroot Disease in Cruciferous Crops

Advanced Science, EarlyView.
This study uncovers an unusual RNAi pathway in the rhizarian pathogen Plasmodiophora brassicae. In the absence of Dicer, a Drosha‐like RNase III protein supports the biogenesis of predominant 21‐nt small RNAs, and two Argonaute proteins mediate small RNA‐guided silencing.
Xiong Zhang, Yupo Wu, Jiachang Hou, Lixia Gao, Longqi Pan, Cuicui Shen, Chuanji Zhao, Feng Gao, Yi Zhang, Li Ren, Xiaoping Fang, Xiaoling Dun, Shengyi Liu, Lijiang Liu   +13 more
wiley   +1 more source

Positive-Strand RNA Viral RdRps: From Structure Conservation and Activity Assay to Drug Development

Molecules
Positive-strand RNA viruses represented by SARS-CoV-2 and DENV spread globally with high infectivity and frequent mutations, posing a severe threat to human health. However, specific and effective antiviral drugs remain limited.
Siyuan Zhao, Ziyu Lin, Minglian Wang
doaj   +1 more source

Expression and purification of tag-free SARS-CoV-2 RNA-dependent RNA polymerase in Escherichia coli

STAR Protocols, 2021
Summary: The RNA-dependent-RNA polymerase (RdRp) from SARS-CoV-2 is an important drug target because it is responsible for viral RNA genome replication. Efficient production of recombinant RdRp is important in screening antivirals to treat COVID-19. Here,
Tyler L. Dangerfield, Nathan Z. Huang, Kenneth A. Johnson   +2 more
doaj   +1 more source

A Dual‐Membrane Biomimetic Nanoplatform Enables Triple‐Modal Therapy Against SARS‐CoV‐2 Through Viral Decoy, Inflammation Neutralizing, and Intracellular RNAi

Advanced Science, EarlyView.
[A&T]MLN is a triple‐modal nanoplatform with siRNA‐loaded LN coated with hybrid ACE2/macrophage membrane. It blocks viral entry, neutralizes IL‐6/IL‐1β/TNF‐α, and delivers siRNA to suppress viral replication. In a murine lung injury model, it attenuates inflammation, offering a multi‐pronged strategy against SARS‐CoV‐2 variants and hyperinflammation ...
Hui Li, Bohan Zhang, Chao Shang, Yi Li, Te Zhao, Shengya Liu, Dan Liu, Junwei Che, Cuiling Zhang, Pinghui Wu, Wenjie Yang, Junjie Ding, Yuhua Ran, Yanan Zhai, Haihua Xiao, Jing Gao, Xiao Li, Chunsheng Gao, Lin Li, Zhiping Li   +19 more
wiley   +1 more source

RNA polymerase I-specific subunits promote polymerase clustering to enhance the rRNA gene transcription cycle. [PDF]

, 2011
RNA polymerase I (Pol I) produces large ribosomal RNAs (rRNAs). In this study, we show that the Rpa49 and Rpa34 Pol I subunits, which do not have counterparts in Pol II and Pol III complexes, are functionally conserved using heterospecific ...
Gadal, O., Leger-Silvestre, Isabelle, Ostermaier, M.K., Panov, Konstantin; id_orcid, Panov, Kostya I, Schultz, P., Leger-Silvestre, I. (I), Olivier Gadal, Jorge Pérez-Fernández, Albert, Benjamin, Leger-Silvestre, I., Ostermaier, Martin K, Kostya I. Panov, Perez-Fernandez, J., Normand, C. (C), Schultz, Patrick, Schultz, P. (Patrick), Panov, Kostya I., Patrick Schultz, Gadal, O. (O), Normand, Christophe, Martin K. Ostermaier, Normand, C., Zomerdijk, Joost C.B.M., Albert, B. (B), Perez-Fernandez, J. (J), Ostermaier, M. (M) K. (K), Ostermaier, Martin K., Christophe Normand, Zomerdijk, Joost C B M, Gadal, Olivier, Joost C.B.M. Zomerdijk, Zomerdijk, J.C., Perez-Fernandez, Jorge, Isabelle Léger-Silvestre, Albert, B., Panov, K. (K) I. (I), Zomerdijk, Joost C. B. M., Benjamin Albert, Zomerdijk, J. (J) C. (C)   +39 more
core   +1 more source

Identifying Therapeutic Compounds Targeting RNA-Dependent-RNA-Polymerase of Sars-Cov-2 [PDF]

, 2020
COVID-19 emerged as the biggest threat of this century for mankind and later it spread across the globe through human to human transmission. Scientists rushed to understand the structure and mechanism of the virus so that antiviral drugs or vaccines to ...
Muhammad, Roomi, Yaser, Khan, Muhammad, Mahmood   +2 more
core   +1 more source

Combinatorial library screening of quinadoline B derivatives against SARS-CoV-2 RNA-dependent RNA polymerase [PDF]

, 2021
The unprecedented global health threat of SARS-CoV-2 has sparked a continued interest to discover novel anti-COVID-19 agents. To this end, we present here a computer-based protocol for identifying potential compounds targeting RNA-dependent RNA ...
Kin Israel , Notarte, Mark Tristan , Quimque, simone, brogi, Vincenzo, Calderone, Jeremiah Gabriel, Africa, Allan Patrick , Macabeo, Jenina Beatriz, Hernandez, Sophia Morgan, Tan   +7 more
core   +2 more sources