Human Leukemic Cells: RNA‐Directed DNA Polymerase [PDF]
European Journal of Biochemistry, 1974 The enzyme(s) RNA‐dependent DNA polymerase (reverse transcriptase) has been isolated from human lymphocytic cell lines which have been derived from patients with leukemia, infectious mononucleosis, and from normal healthy donors. The reverse transcriptase was purified through high‐speed glycerol gradients, and by DEAE‐cellulose and phosphocellulose ...
L.S. Desai +3 more
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In vitro transcription of theavian oncornavirus genome by the RNA-directed DNA polymerase: analysis of DNA transcripts synthesized in reconstructed enzymatic reactions [PDF]
Journal of Virology, 1977 Marc S. Collett, Anthony J. Faras
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Unwinding-like activity associated with avian retrovirus RNA-directed DNA polymerase [PDF]
Journal of Virology, 1978 The avian retrovirus RNA-directed DNA polymerase contains an activity that is capable of removing hydrogen bonds from duplex nucleic acid molecules. This "unwinding-like" activity appears to be specific in its action, affecting RNA.DNA and DNA.DNA duplex molecules but not RNA.RNA duplexes.
M. Collett, J. Leis, M. Smith, A. Faras
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Characterization of endogenous RNA-directed DNA polymerase activity of reticuloendotheliosis viruses [PDF]
Journal of Virology, 1975 Reticuloendotheliosis viruses (REV) contain an endogenous RNA-directed DNA polymerase activity. The endogenous DNA polymerase activity can be elicited in purified preparations of REV by treatment with nonionic detergents. The enzyme activity has a strong preference for manganous ions.
C. Kang
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Effect of UV Light on RNA-Directed DNA Polymerase Activity of Murine Oncornaviruses [PDF]
Journal of Virology, 1975 The UV inactivation of RNA-directed DNA polymerase activity of Rauscher leukemia virus was shown to be due to damage to the protein. The UV dose resulting in 37% survival of viral polymerase activity at 254 nm was 2.4 × 10 4 to 3.1 × 10 4 ergs/mm 2 .
G. G. Lovinger +3 more
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RNA-mediated in vivo Directed Evolution in Yeast
Bio-Protocol, 2022 Directed evolution is a powerful approach to obtain genetically-encoded sought-for traits. Compared to the prolonged adaptation regimes to mutations occurring under natural selection, directed evolution unlocks rapid screening and selection of mutants ...
Emil Jensen, Michael Jensen
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Initiation of DNA synthesis by the avian oncornavirus RNA-directed DNA polymerase: tryptophan tRNA as the major species of primer RNA [PDF]
Journal of Virology, 1976 William R. Folk, Anthony J. Faras
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Endonuclease activity of purified RNA-directed DNA polymerase from avian myeloblastosis virus.
Journal of Biological Chemistry, 1979 Highly purified preparations of RNA-directed DNA polymerase from avian myeloblastosis virus (AMV) contain a Mn2+-activated endonuclease activity capable of nicking supercoiled DNA. This endonuclease activity co-sediments in glycerol gradients with the alphabeta form of AMV DNA polymerase, and co-chromatographs with DNA polymerase activity on DEAE ...
M. Golomb, D. Grandgenett
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eLife, 2022 In plants, selfish genetic elements, including retrotransposons and DNA viruses, are transcriptionally silenced by RNA-directed DNA methylation. Guiding the process are short interfering RNAs (siRNAs) cut by DICER-LIKE 3 (DCL3) from double-stranded ...
Andrew Loffer +5 more
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SHH1, a homeodomain protein required for DNA methylation, as well as RDR2, RDM4, and chromatin remodeling factors, associate with RNA polymerase IV. [PDF]
PLoS Genetics, 2011 DNA methylation is an evolutionarily conserved epigenetic modification that is critical for gene silencing and the maintenance of genome integrity. In Arabidopsis thaliana, the de novo DNA methyltransferase, domains rearranged methyltransferase 2 (DRM2),
Julie A Law +3 more
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