Arabidopsis RNA Polymerase IV generates 21-22 nucleotide small RNAs that can participate in RNA-directed DNA methylation and may regulate genes [PDF]
bioRxiv, 2019 The plant-specific RNA Polymerase IV (Pol IV) transcribes heterochromatic regions, including many transposable elements, with the well-described role of generating 24 nucleotide (nt) small interfering RNAs (siRNAs).
Kaushik Panda +2 more
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Characterization of endogenous RNA-directed DNA polymerase activity of reticuloendotheliosis viruses [PDF]
Journal of Virology, 1975 Reticuloendotheliosis viruses (REV) contain an endogenous RNA-directed DNA polymerase activity. The endogenous DNA polymerase activity can be elicited in purified preparations of REV by treatment with nonionic detergents. The enzyme activity has a strong preference for manganous ions.
C. Kang
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Endonuclease activity of purified RNA-directed DNA polymerase from avian myeloblastosis virus.
Journal of Biological Chemistry, 1979 Highly purified preparations of RNA-directed DNA polymerase from avian myeloblastosis virus (AMV) contain a Mn2+-activated endonuclease activity capable of nicking supercoiled DNA. This endonuclease activity co-sediments in glycerol gradients with the alphabeta form of AMV DNA polymerase, and co-chromatographs with DNA polymerase activity on DEAE ...
M Golomb, Duane P. Grandgenett
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A Protein Complex Required for Polymerase V Transcripts and RNA- Directed DNA Methylation in Arabidopsis [PDF]
Current Biology, 2010 DNA methylation is an epigenetic modification associated with gene silencing. In Arabidopsis, DNA methylation is established by DOMAINS REARRANGED METHYLTRANSFERASE 2 (DRM2), which is targeted by small interfering RNAs through a pathway termed RNA-directed DNA methylation (RdDM).
James A. Wohlschlegel +7 more
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Group Specific Antigen and RNA-Directed DNA Polymerase Activity in Normal Baboon Placenta
Experimental Biology and Medicine, 1973 SummaryC-Type particles were observed in normal baboon placentas at various stages of gestation. This tissue was found to have interspecies specific antigens of the C-type RNA tumor viruses and RNA-directed DNA polymerase activity that could not be inhibited by antisera prepared against the previously recognized reverse transcriptases of several RNA ...
James E. Strickland +6 more
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In Vitro Transcription of 70 S RNA by the RNA-Directed DNA Polymerase of Rous Sarcoma Virus: Lack of Influence of RNase H [PDF]
Journal of Virology, 1976 Marc S. Collett, Anthony J. Faras
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In vitro transcription of theavian oncornavirus genome by the RNA-directed DNA polymerase: analysis of DNA transcripts synthesized in reconstructed enzymatic reactions [PDF]
Journal of Virology, 1977 Marc S. Collett, Anthony J. Faras
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Initiation of DNA synthesis by the avian oncornavirus RNA-directed DNA polymerase: tryptophan tRNA as the major species of primer RNA [PDF]
Journal of Virology, 1976 William R. Folk, Anthony J. Faras
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RNA-mediated in vivo Directed Evolution in Yeast
Bio-Protocol, 2022 Directed evolution is a powerful approach to obtain genetically-encoded sought-for traits. Compared to the prolonged adaptation regimes to mutations occurring under natural selection, directed evolution unlocks rapid screening and selection of mutants ...
Emil Jensen, Michael Jensen
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eLife, 2022 In plants, selfish genetic elements, including retrotransposons and DNA viruses, are transcriptionally silenced by RNA-directed DNA methylation. Guiding the process are short interfering RNAs (siRNAs) cut by DICER-LIKE 3 (DCL3) from double-stranded ...
Andrew Loffer +5 more
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