Results 81 to 90 of about 1,873,837 (361)

Organ‐specific redox imbalances in spinal muscular atrophy mice are partially rescued by SMN antisense oligonucleotides

FEBS Letters, EarlyView.
We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley   +1 more source

SPOT-Seq-RNA: Predicting protein-RNA complex structure and RNA-binding function by fold recognition and binding affinity prediction [PDF]

, 2014
RNA-binding proteins (RBPs) play key roles in RNA metabolism and post-transcriptional regulation. Computational methods have been developed separately for prediction of RBPs and RNA-binding residues by machine-learning techniques and prediction of ...
A Castello, E Faraggi, E Faraggi, E Faraggi, FC Bernstein, H Zhao, H Zhao, HY Zhao, HY Zhou, HY Zhou, I Tuszynska, J Soding, L Perez-Cano, M Nowotny, NG Tsvetanova, O Dor, P Setny, RR Walia, S Liu, S Zheng, SF Altschul, T Puton, T Scherrer, Y Yang, Y Yang, Y Zhou, YQ Zhou   +26 more
core   +2 more sources

Alternative polyadenylation and RNA-binding proteins [PDF]

Journal of Molecular Endocrinology, 2016
Our understanding of the extent of microRNA-based gene regulation has expanded in an impressive pace over the past decade. Now, we are beginning to better appreciate the role of 3′-UTR (untranslated region) cis-elements which harbor not only microRNA but also RNA-binding protein (RBP) binding sites that have significant effect on the stability and ...
openaire   +3 more sources

Transferrin receptor 1‐mediated iron uptake supports thermogenic activation in human cervical‐derived adipocytes

FEBS Letters, EarlyView.
In this study, we found that human cervical‐derived adipocytes maintain intracellular iron level by regulating the expression of iron transport‐related proteins during adrenergic stimulation. Melanotransferrin is predicted to interact with transferrin receptor 1 based on in silico analysis.
Rahaf Alrifai, Mizuki Seo, Gyath Karadsheh, Fachrur Rizal Mahendra, Máté Á Demény, Ferenc Győry, János András Mótyán, László Fésüs, Endre Kristóf, Rini Arianti   +9 more
wiley   +1 more source

Iron enhances the binding rates and translational efficiency of iron responsive elements (IREs) mRNA with initiation factor eIF4F.

PLoS ONE, 2021
Interaction of iron responsive elements (IRE) mRNA with the translational machinery is an early step critical in the initiation of protein synthesis.
Mateen A Khan, Artem V Domashevskiy
doaj   +1 more source

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Rationalizing the effects of RNA modifications on protein interactions

Molecular Therapy: Nucleic Acids
RNA modifications play a crucial role in regulating gene expression by altering RNA structure and modulating interactions with RNA-binding proteins (RBPs).
Andrea Vandelli, Laura Broglia, Alexandros Armaos, Riccardo Delli Ponti, Gian Gaetano Tartaglia   +4 more
doaj   +1 more source

Northwestern Blot of Protein-RNA Interaction from Young Rice Panicles

Bio-Protocol, 2013
The northwestern assay is employed to study the interaction between protein and RNA. The RNA binding proteins tend to bind to different kinds of RNA through either known domains or unknown sequences of proteins.
Saminathan Thangasamy, Guang-Yuh Jauh
doaj   +1 more source

A creature with a hundred waggly tails: intrinsically disordered proteins in the ribosome [PDF]

, 2013
This article is made available for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source.
Dunker, A. Keith, Kurgan, Lukasz, Mizianty, Marcin J., Oldfield, Christopher J., Peng, Zhenling, Uversky, Vladmir N., Xue, Bin   +6 more
core   +2 more sources

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source