Results 251 to 260 of about 29,994 (285)
Quantifying the RNA cap epitranscriptome reveals novel caps in cellular and viral RNA [PDF]
Nucleic Acids Research, 2019 Abstract Chemical modification of transcripts with 5′ caps occurs in all organisms. Here, we report a systems-level mass spectrometry-based technique, CapQuant, for quantitative analysis of an organism's cap epitranscriptome. The method was piloted with 21 canonical caps—m7GpppN, m7GpppNm, GpppN, GpppNm, and m2,2,7GpppG—and 5 ...
Jin Wang +2 more
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Journal of Physical Chemistry A, 2017 In this study, the electrostatically embedded generalized molecular fractionation with conjugate caps (concaps) method (EE-GMFCC) was employed for efficient linear-scaling quantum mechanical (QM) calculation of total energies of RNAs.
Xinsheng Jin, John Z H Zhang, Xiao He
exaly +2 more sources
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NAD-capped RNAs – a redox cofactor meets RNA
Trends in Biochemical Sciences, 2023RNA modifications immensely expand the diversity of the transcriptome, thereby influencing the function, localization, and stability of RNA. One prominent example of an RNA modification is the eukaryotic cap located at the 5' terminus of mRNAs. Interestingly, the redox cofactor NAD can be incorporated into RNA by RNA polymerase in vitro.
Maik Wolfram-Schauerte, Katharina Höfer
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Chemical Route to the Capped RNAs
Nucleosides, Nucleotides & Nucleic Acids, 2004Eukaryotic and viral messenger RNAs contain a CAP structure that plays an important role in the initiation of translation and several other cellular processes that involve mRNAs. In this paper, we report a convenient chemical approach to the preparation of milligram quantities of short, capped RNA oligonucleotides, which overcomes some of the ...
I I, Koukhareva, A V, Lebedev
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Synthesis of 5′-NAD-Capped RNA
Bioconjugate Chemistry, 2016In prokaryotic organisms, certain regulatory RNAs have recently been found to be linked to the ubiquitous redox cofactor nicotinamide adenine dinucleotide (NAD) at their 5'-ends. Biochemical and structural investigations of this new caplike RNA modification require synthetic access to pure NAD-RNA. Here we report a chemoenzymatic approach to generate 5'
Höfer, K. +3 more
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Good cap/bad cap: how the cap-binding complex determines RNA fate
Nature Structural & Molecular Biology, 2014Every RNA polymerase II transcript receives a 5′-end 7-methylguanosine (m7G) cap, which is rapidly bound by the nuclear cap–binding complex (CBC). Two recent studies now reveal that the CBC associates with a variety of effector proteins that enable it to interrogate nascent RNA, discriminating between distinct RNA subclasses and routing them either ...
Müller-McNicoll, M., Neugebauer, K.
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A General RNA-Capping Ribozyme Retains Stereochemistry during Cap Exchange
Journal of the American Chemical Society, 2006Numerous natural and artificial ribozymes have been shown to facilitate reactions that invert stereochemistry. Here, we demonstrate that an RNA-capping ribozyme retains stereochemistry at a phosphorus reaction center. The ribozyme synthesizes a broad range of 5'-5' RNA caps by exchanging phosphate groups around the alpha-phosphate found at the 5 ...
Hani S, Zaher, Peter J, Unrau
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Co-transcriptional capping using an RNA capping enzyme-T7 RNA polymerase fusion protein
2023Abstract mRNA vaccines and therapeutics are highly effective and can be developed and manufactured with a relatively short lead time. Here we report an all-enzyme platform to generate capped synthetic RNA in a one-step process based on an RNA capping enzyme-T7 RNA polymerase fusion protein. Under standard in vitro transcription reaction
S. Hong Chan +5 more
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The uncharted territory of NAD+-capped RNA
Trends in Cell BiologyThe critical redox cofactor NAD+ was recently reported to serve as an RNA cap in both eukaryotes and prokaryotes. However, its reversible regulation and biological functions remain unclear. Here, we provide insights into its discovery, capping and decapping mechanisms, for further discovery of their potential functional implications.
Quan Ma +3 more
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