RNA Cap Methyltransferase Activity Assay [PDF]
Bio-Protocol, 2018 Methyltransferases that methylate the guanine-N7 position of the mRNA 5’ cap structure are ubiquitous among eukaryotes and commonly encoded by viruses. Here we provide a detailed protocol for the biochemical analysis of RNA cap methyltransferase activity
Jackson Trotman, Daniel Schoenberg
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CK2 phosphorylation of CMTR1 promotes RNA cap formation and influenza virus infection [PDF]
Cell ReportsSummary: The RNA cap methyltransferase CMTR1 methylates the first transcribed nucleotide of RNA polymerase II transcripts, impacting gene expression mechanisms, including during innate immune responses.
Radoslaw Lukoszek +8 more
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Structural basis of RNA cap modification by SARS-CoV-2 [PDF]
Nature Communications, 2020 Specific non-structural proteins (nsp) of SARS coronaviruses are involved in methylation of virally encoded mRNAs to mimic cellular mRNAs for protection against host innate immune restriction. Here, the authors present a high resolution structure of SARS-
Thiruselvam Viswanathan +11 more
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mRNA Cap Methyltransferase, RNMT-RAM, Promotes RNA Pol II-Dependent Transcription
Cell Reports, 2018 Summary: mRNA cap addition occurs early during RNA Pol II-dependent transcription, facilitating pre-mRNA processing and translation. We report that the mammalian mRNA cap methyltransferase, RNMT-RAM, promotes RNA Pol II transcription independent of mRNA ...
Dhaval Varshney +5 more
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N7-Methylation of the Coronavirus RNA Cap Is Required for Maximal Virulence by Preventing Innate Immune Recognition [PDF]
MBio, 2022 Zhen Zhang, Qianyun Liu, Roland Züst
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CAP-MAP: cap analysis protocol with minimal analyte processing, a rapid and sensitive approach to analysing mRNA cap structures [PDF]
Open Biology, 2020 Eukaryotic messenger RNA (mRNA) is modified by the addition of an inverted guanosine cap to the 5′ triphosphate. The cap guanosine and initial transcribed nucleotides are further methylated by a series of cap methyltransferases to generate the mature cap
Alison Galloway +5 more
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RNA-Protein Interactome at the Hepatitis E Virus Internal Ribosome Entry Site
Microbiology Spectrum, 2023 Multiple processes exist in a cell to ensure continuous production of essential proteins either through cap-dependent or cap-independent translation processes. Viruses depend on the host translation machinery for viral protein synthesis.
Shiv Kumar +11 more
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Translation of Plant RNA Viruses
Viruses, 2021 Plant RNA viruses encode essential viral proteins that depend on the host translation machinery for their expression. However, genomic RNAs of most plant RNA viruses lack the classical characteristics of eukaryotic cellular mRNAs, such as mono-cistron, 5′
Guowei Geng +7 more
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Synthetic Abortive HIV-1 RNAs Induce Potent Antiviral Immunity
Frontiers in Immunology, 2020 Strong innate and adaptive immune responses are paramount in combating viral infections. Dendritic cells (DCs) detect viral infections via cytosolic RIG-I like receptors (RLRs) RIG-I and MDA5 leading to MAVS-induced immunity.
Melissa Stunnenberg +6 more
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Multiplicity of 5' cap structures present on short RNAs. [PDF]
PLoS ONE, 2014 Most RNA molecules are co- or post-transcriptionally modified to alter their chemical and functional properties to assist in their ultimate biological function.
Rehab F Abdelhamid +7 more
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