Results 11 to 20 of about 134,303 (299)
Captivating Perplexities of Spinareovirinae 5′ RNA Caps [PDF]
Viruses, 2021 RNAs with methylated cap structures are present throughout multiple domains of life. Given that cap structures play a myriad of important roles beyond translation, such as stability and immune recognition, it is not surprising that viruses have adopted ...
Justine Kniert +2 more
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Enzymatic synthesis of RNAs capped with nucleotide analogues reveals the molecular basis for substrate selectivity of RNA capping enzyme: impacts on RNA metabolism. [PDF]
PLoS ONE, 2013 RNA cap binding proteins have evolved to specifically bind to the N7-methyl guanosine cap structure found at the 5' ends of eukaryotic mRNAs. The specificity of RNA capping enzymes towards GTP for the synthesis of this structure is therefore crucial for ...
Moheshwarnath Issur +3 more
doaj +4 more sources
Quantifying the RNA cap epitranscriptome reveals novel caps in cellular and viral RNA. [PDF]
Nucleic Acids Res, 2019 Abstract Chemical modification of transcripts with 5′ caps occurs in all organisms. Here, we report a systems-level mass spectrometry-based technique, CapQuant, for quantitative analysis of an organism's cap epitranscriptome. The method was piloted with 21 canonical caps—m7GpppN, m7GpppNm, GpppN, GpppNm, and m2,2,7GpppG—and 5 ...
Wang J +15 more
europepmc +6 more sources
Synthesis of 5′-Thiamine-Capped RNA [PDF]
Molecules, 2020 RNA 5′-modifications are known to extend the functional spectrum of ribonucleotides. In recent years, numerous non-canonical 5′-modifications, including adenosine-containing cofactors from the group of B vitamins, have been confirmed in all kingdoms of life.
Marvin Möhler +2 more
openaire +4 more sources
Enzymology of RNA cap synthesis [PDF]
WIREs RNA, 2010 AbstractThe 5′ guanine‐N7 methyl cap is unique to cellular and viral messenger RNA (mRNA) and is the first co‐transcriptional modification of mRNA. The mRNA cap plays a pivotal role in mRNA biogenesis and stability, and is essential for efficient splicing, mRNA export, and translation.
Agnidipta, Ghosh, Christopher D, Lima
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RNMT-dependent RNA cap methylation in health and disease. [PDF]
Biochem JRNA cap formation on RNA polymerase II transcripts is regulated by cellular signalling pathways during development and differentiation, adaptive and innate immune responses, during the cell cycle and in response to oncogene deregulation. Here, we discuss
Mills JG, Hepburn LA, Cowling VH.
europepmc +2 more sources
Deciphering the RNA capping process in bacteria [PDF]
Proceedings of the National Academy of Sciences, 2020 RNA capping in eukaryotes has been studied since the 1970s, starting with the discovery of 5′ 7-methylguanylate caps in the Shatkin laboratory (1). That capping mechanism involves a pause during transcription elongation that allows the recruitment of specialized capping enzymes to modify the 5′ end of the nascent RNA.
Jonathan, Jagodnik, Richard L, Gourse
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Identification of gemin5 as a novel 7-methylguanosine cap-binding protein. [PDF]
PLoS ONE, 2009 A unique attribute of RNA molecules synthesized by RNA polymerase II is the presence of a 7-methylguanosine (m(7)G) cap structure added co-transcriptionally to the 5' end.
Shelton S Bradrick, Matthias Gromeier
doaj +1 more source
The mechanism of RNA capping by SARS-CoV-2 [PDF]
Nature, 2022 Abstract The SARS-CoV-2 RNA genome contains a 5′-cap that facilitates translation of viral proteins, protection from exonucleases and evasion of the host immune response1-4. How this cap is made is not completely understood. Here, we reconstitute the SARS-CoV-2 7MeGpppA2′-O-Me-RNA cap using virally encoded non-structural proteins (nsps).
Gina J. Park +11 more
openaire +4 more sources
A Novel NAD-RNA Decapping Pathway Discovered by Synthetic Light-Up NAD-RNAs
Biomolecules, 2020 The complexity of the transcriptome is governed by the intricate interplay of transcription, RNA processing, translocation, and decay. In eukaryotes, the removal of the 5’-RNA cap is essential for the initiation of RNA degradation.
Florian Abele +8 more
doaj +1 more source