Results 1 to 10 of about 1,248,361 (297)
Cleavage-based signal amplification of RNA [PDF]
Nature Communications, 2013 RNA detection has become an integral part of current biomedical research. Up to now, the reverse transcription-PCR has been the most practical method to detect mRNA targets. However, RNA detection by reverse transcription-PCR requires sophisticated equipment and it is highly sensitive to contamination with genomic DNA.
Yongyun, Zhao, Li, Zhou, Zhuo, Tang
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RNA secondary structure and nucleotide composition of the conserved hallmark sequence of Leishmania SIDER2 retroposons are essential for endonucleolytic cleavage and mRNA degradation. [PDF]
PLoS ONE, 2017 We have reported previously that Short Interspersed Degenerate Retroposons of the SIDER2 subfamily, largely located within 3'UTRs of Leishmania transcripts, promote rapid turnover of mRNAs through endonucleolytic cleavage within the highly conserved ...
Hiva Azizi +6 more
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Heliyon, 2023 Cuproptosis is a novel form of programmed cell death. The role and mechanism of cuproptosis-related genes in prostate adenocarcinoma have not been fully understood. In this study, a series of bioinformatic analyses were performed.
Shuyuan Xiao, Weiyang Lou
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Transfer RNA Cleavages by Onconase Reveal Unusual Cleavage Sites [PDF]
Journal of Biological Chemistry, 2006 Onconase, a protein from amphibian eggs and a homologue of pancreatic ribonuclease (RNase) superfamily, is cytotoxic, exhibits antitumor and antiviral activity, and is in phase III clinical trials. It has been shown to predominantly target cellular tRNA on its entry into mammalian cells (Saxena, S. K., Sirdeshmukh, R., Ardelt, W., Mikulski, S.
Avvaru N, Suhasini, Ravi, Sirdeshmukh
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Enzymatic cleavage of RNA by RNA
Trends in Biochemical Sciences, 1986 The discovery and characterization of the catalytic RNA subunit of the enzyme ribonuclease P ofEscherichia coli is described.
Sidney Altman +3 more
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Evolution of the R2 retrotransposon ribozyme and its self-cleavage site. [PDF]
PLoS ONE, 2013 R2 is a non-long terminal repeat retrotransposon that inserts site-specifically in the tandem 28S rRNA genes of many animals. Previously, R2 RNA from various species of Drosophila was shown to self-cleave from the 28S rRNA/R2 co-transcript by a hepatitis
Danna G Eickbush +2 more
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Bioengineering, 2023 Conserved omicron RNA (COR) is a 40 base long 99.9% conserved sequence in SARS-CoV-2 Omicron variant, predicted to form a stable stem loop, the targeted cleavage of which can be an ideal next step in controlling the spread of variants.
Lindy G. Karrer +4 more
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Nucleic Acids Research, 1987 Using complementary chimeric oligonucleotides containing deoxyribonucleotides and 2'-O-methylribonucleotides (1), enzymatically synthesized RNA (90 mer) were cleaved at a single site with Escherichia coli RNaseH, either at a hairpin loop or at a stem region.
S, Shibahara +5 more
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Binding and cleavage of CRISPR RNA by Cas6 [PDF]
RNA, 2010 The CRISPR–Cas system provides many prokaryotes with acquired resistance to viruses and other mobile genetic elements. The core components of this defense system are small, host-encoded prokaryotic silencing (psi)RNAs and Cas (CRISPR-associated) proteins.
Jason, Carte +4 more
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Endonucleolytic RNA cleavage by a eukaryotic exosome [PDF]
Nature, 2008 The exosome is a major eukaryotic nuclease located in both the nucleus and the cytoplasm that contributes to the processing, quality control and/or turnover of a large number of cellular RNAs. This large macromolecular assembly has been described as a 3'-->5' exonuclease and shown to contain a nine-subunit ring structure evolutionarily related to ...
Lebreton, Alice +3 more
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