RNase P-Mediated Sequence-Specific Cleavage of RNA by Engineered External Guide Sequences
Biomolecules, 2015 The RNA cleavage activity of RNase P can be employed to decrease the levels of specific RNAs and to study their function or even to eradicate pathogens. Two different technologies have been developed to use RNase P as a tool for RNA knockdown.
Ger J M Pruijn
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RNA modifications can affect RNase H1-mediated PS-ASO activity
Molecular Therapy: Nucleic Acids, 2022 Phosphorothioate modified antisense oligonucleotides (PS-ASOs) can reduce gene expression through hybridization to target RNAs and subsequent cleavage by RNase H1.
Katelyn A. Doxtader Lacy +3 more
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Креативная хирургия и онкология, 2020 Background. With numerous extraction protocols for total RNA and RNA fractions, like microRNA (miRNA) and long non-coding RNA (lncRNA), available for various cell and tissue types, obtaining a high quantitative and qualitative yield from some special ...
I. F. Gareev +3 more
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SARS coronavirus nsp1 protein induces template-dependent endonucleolytic cleavage of mRNAs: viral mRNAs are resistant to nsp1-induced RNA cleavage. [PDF]
PLoS Pathogens, 2011 SARS coronavirus (SCoV) nonstructural protein (nsp) 1, a potent inhibitor of host gene expression, possesses a unique mode of action: it binds to 40S ribosomes to inactivate their translation functions and induces host mRNA degradation.
Cheng Huang +5 more
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STAR Protocols, 2022 Summary: Here, we provide an optimized RNA-induced silencing complex (RISC) assembly and cleavage protocol in vitro without using radiolabeled RNA. The protocol is useful to characterize the biochemical properties of the RISC. We describe the preparation
Yajie Gao +3 more
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Biomolecules, 2022 Prokaryotic Argonautes (pAgos) from mesophilic bacteria are attracting increasing attention for their genome editing potential. So far, it has been reported that KmAgo from Kurthia massiliensis can utilize DNA and RNA guide of any sequence to effectively
Xiaoman Jiang +3 more
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RNA secondary structure and nucleotide composition of the conserved hallmark sequence of Leishmania SIDER2 retroposons are essential for endonucleolytic cleavage and mRNA degradation. [PDF]
PLoS ONE, 2017 We have reported previously that Short Interspersed Degenerate Retroposons of the SIDER2 subfamily, largely located within 3'UTRs of Leishmania transcripts, promote rapid turnover of mRNAs through endonucleolytic cleavage within the highly conserved ...
Hiva Azizi +6 more
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A smart deoxyribozyme-based fluorescent sensor for in vitro detection of androgen receptor mRNA
Beilstein Journal of Organic Chemistry, 2020 Nowadays a variety of biosensors are widely used in different fields, including biomedical diagnostics and self-testing. Nucleic acid-based biosensors are typically applied to detect another nucleic acid, proteins, ions, and several other types of ...
Ekaterina A. Bryushkova +2 more
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Type III-A CRISPR-Cas Csm Complexes: Assembly, Periodic RNA Cleavage, DNase Activity Regulation, and Autoimmunity [PDF]
Molecular Cell, 2019 Ning Jia, Charlie Y Mo, Chongyuan Wang
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Programmable RNA Cleavage and Recognition by a Natural CRISPR-Cas9 System from Neisseria meningitidis [PDF]
Molecular Cell, 2018 Zhonggang Hou
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