Results 21 to 30 of about 1,248,361 (297)
In vitro RNA Cleavage Assays to Characterize IRE1-dependent RNA Decay [PDF]
Bio-Protocol, 2019 The kinase/RNase IRE1 is a key effector of the cellular response to endoplasmic reticulum stress. The RNase activity of IRE1 can be measured in cells or in the test tube.
G. Karagöz +3 more
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Critical factors for precise and efficient RNA cleavage by RNase Y in Staphylococcus aureus. [PDF]
PLoS GeneticsCellular processes require precise and specific gene regulation, in which continuous mRNA degradation is a major element. The mRNA degradation mechanisms should be able to degrade a wide range of different RNA substrates with high efficiency, but should ...
Alexandre Le Scornet +14 more
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RNase P-Mediated Sequence-Specific Cleavage of RNA by Engineered External Guide Sequences
Biomolecules, 2015 The RNA cleavage activity of RNase P can be employed to decrease the levels of specific RNAs and to study their function or even to eradicate pathogens. Two different technologies have been developed to use RNase P as a tool for RNA knockdown.
Merel Derksen +2 more
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RNA modifications can affect RNase H1-mediated PS-ASO activity
Molecular Therapy: Nucleic Acids, 2022 Phosphorothioate modified antisense oligonucleotides (PS-ASOs) can reduce gene expression through hybridization to target RNAs and subsequent cleavage by RNase H1.
Katelyn A. Doxtader Lacy +3 more
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Novel endoribonucleases as central players in various pathways of eukaryotic RNA metabolism [PDF]
, 2010 For a long time it has been assumed that the decay of RNA in eukaryotes is mainly carried out by exoribonucleases, which is in contrast to bacteria, where endoribonucleases are well documented to initiate RNA degradation.
Tomecki, Rafał, Dziembowski, Andrzej
core +3 more sources
SARS coronavirus nsp1 protein induces template-dependent endonucleolytic cleavage of mRNAs: viral mRNAs are resistant to nsp1-induced RNA cleavage. [PDF]
PLoS Pathogens, 2011 SARS coronavirus (SCoV) nonstructural protein (nsp) 1, a potent inhibitor of host gene expression, possesses a unique mode of action: it binds to 40S ribosomes to inactivate their translation functions and induces host mRNA degradation.
Cheng Huang +5 more
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STAR Protocols, 2022 Summary: Here, we provide an optimized RNA-induced silencing complex (RISC) assembly and cleavage protocol in vitro without using radiolabeled RNA. The protocol is useful to characterize the biochemical properties of the RISC. We describe the preparation
Yajie Gao +3 more
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A smart deoxyribozyme-based fluorescent sensor for in vitro detection of androgen receptor mRNA
Beilstein Journal of Organic Chemistry, 2020 Nowadays a variety of biosensors are widely used in different fields, including biomedical diagnostics and self-testing. Nucleic acid-based biosensors are typically applied to detect another nucleic acid, proteins, ions, and several other types of ...
Ekaterina A. Bryushkova +2 more
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Biomolecules, 2022 Prokaryotic Argonautes (pAgos) from mesophilic bacteria are attracting increasing attention for their genome editing potential. So far, it has been reported that KmAgo from Kurthia massiliensis can utilize DNA and RNA guide of any sequence to effectively
Xiaoman Jiang +3 more
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