Identification of human mitochondrial RNA cleavage sites and candidate RNA processing factors [PDF]
BMC Biology, 2022 Background The human mitochondrial genome is transcribed as long strands of RNA containing multiple genes, which require post-transcriptional cleavage and processing to release functional gene products that play vital roles in cellular energy production.
Guillermo Carbajosa +2 more
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Feature selection for RNA cleavage efficiency at specific sites using the LASSO regression model in Arabidopsis thaliana [PDF]
BMC Bioinformatics, 2021 Background RNA degradation is important for the regulation of gene expression. Despite the identification of proteins and sequences related to deadenylation-dependent RNA degradation in plants, endonucleolytic cleavage-dependent RNA degradation has not ...
Daishin Ueno +4 more
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Molecular basis of target RNA cleavage by Cas13 [PDF]
Nature CommunicationsRNA-targeting CRISPR-Cas13 enzymes are robust RNA knockdown tools with both on-target and collateral cleavage activities. However, to date, the in vivo RNA cleavage mechanisms remain poorly understood.
Joe K. C. Lam +4 more
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A Programmable Eukaryotic Argonaute Nuclease with Dual DNA and RNA Cleavage Activity from Thermophilic Fungus Thermochaetoides thermophila [PDF]
Small ScienceEukaryotic Argonautes (eAgos) are well‐known for their role in utilizing RNA guides to recognize and cleave RNA targets, thereby participating in posttranscriptional regulation and RNA interference.
Zhizhao Chen +4 more
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Temporal dynamics of collateral RNA cleavage by LbuCas13a in human cells [PDF]
Communications BiologyCRISPR-Cas13 exclusively targets RNA. In prokaryotes, Cas13 cleaves both target and non-target RNA indiscriminately upon activation by a specific target RNA, but in eukaryotic cells collateral cleavage activity has been limited.
Jorik Frederik Bot +5 more
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Engineered CRISPR-Cas13a system with enhanced target RNA cleavage activity and reduced collateral activity for therapeutic applications [PDF]
Molecular Therapy: Nucleic AcidsThe CRISPR-Cas13 system exhibits potent RNA cleavage activity and has been widely utilized for RNA-targeting applications. However, its collateral cleavage of bystander RNAs limits in vivo therapeutic applications. In this study, we generated a series of
Wenxia Zhang +9 more
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A detailed protocol for RNA cleavage assay in sympathetic neurons [PDF]
STAR Protocols, 2021 Summary: This protocol illustrates the use of an in vitro assay to study the cleavage of the IMPA1 3′UTR by the endonuclease Ago2 in sympathetic neurons.
Catia Andreassi, Antonella Riccio
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An Ex Vivo Protocol to Assess IRE1α-Dependent RNA Cleavage Using Total RNA Isolated from Mouse Tissues [PDF]
Bio-ProtocolRegulated IRE1-dependent decay (RIDD) is a critical cellular mechanism mediated by the endoplasmic reticulum (ER) stress sensor IRE1α, which cleaves a variety of RNA targets to regulate ER homeostasis.
Mohammad Rashid +3 more
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In vitro RNA Cleavage Assays to Characterize IRE1-dependent RNA Decay [PDF]
Bio-Protocol, 2019 The kinase/RNase IRE1 is a key effector of the cellular response to endoplasmic reticulum stress. The RNase activity of IRE1 can be measured in cells or in the test tube.
G. Karagöz +3 more
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Critical factors for precise and efficient RNA cleavage by RNase Y in Staphylococcus aureus. [PDF]
PLoS GeneticsCellular processes require precise and specific gene regulation, in which continuous mRNA degradation is a major element. The mRNA degradation mechanisms should be able to degrade a wide range of different RNA substrates with high efficiency, but should ...
Alexandre Le Scornet +14 more
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