Identification of human mitochondrial RNA cleavage sites and candidate RNA processing factors [PDF]
BMC Biology, 2022 Background The human mitochondrial genome is transcribed as long strands of RNA containing multiple genes, which require post-transcriptional cleavage and processing to release functional gene products that play vital roles in cellular energy production.
Guillermo Carbajosa +2 more
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Molecular basis of target RNA cleavage by Cas13 [PDF]
Nature CommunicationsRNA-targeting CRISPR-Cas13 enzymes are robust RNA knockdown tools with both on-target and collateral cleavage activities. However, to date, the in vivo RNA cleavage mechanisms remain poorly understood.
Joe K. C. Lam +4 more
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Feature selection for RNA cleavage efficiency at specific sites using the LASSO regression model in Arabidopsis thaliana [PDF]
BMC Bioinformatics, 2021 Background RNA degradation is important for the regulation of gene expression. Despite the identification of proteins and sequences related to deadenylation-dependent RNA degradation in plants, endonucleolytic cleavage-dependent RNA degradation has not ...
Daishin Ueno +4 more
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Temporal dynamics of collateral RNA cleavage by LbuCas13a in human cells [PDF]
Communications BiologyCRISPR-Cas13 exclusively targets RNA. In prokaryotes, Cas13 cleaves both target and non-target RNA indiscriminately upon activation by a specific target RNA, but in eukaryotic cells collateral cleavage activity has been limited.
Jorik Frederik Bot +5 more
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A Programmable Eukaryotic Argonaute Nuclease with Dual DNA and RNA Cleavage Activity from Thermophilic Fungus Thermochaetoides thermophila [PDF]
Small ScienceEukaryotic Argonautes (eAgos) are well‐known for their role in utilizing RNA guides to recognize and cleave RNA targets, thereby participating in posttranscriptional regulation and RNA interference.
Zhizhao Chen +4 more
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The Molecular Architecture for RNA-Guided RNA Cleavage by Cas13a [PDF]
Cell, 2017 Cas13a, a type VI-A CRISPR-Cas RNA-guided RNA ribonuclease, degrades invasive RNAs targeted by CRISPR RNA (crRNA) and has potential applications in RNA technology. To understand how Cas13a is activated to cleave RNA, we have determined the crystal structure of Leptotrichia buccalis (Lbu) Cas13a bound to crRNA and its target RNA, as well as the cryo-EM ...
Yanli Wang +2 more
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Engineered CRISPR-Cas13a system with enhanced target RNA cleavage activity and reduced collateral activity for therapeutic applications [PDF]
Molecular Therapy: Nucleic AcidsThe CRISPR-Cas13 system exhibits potent RNA cleavage activity and has been widely utilized for RNA-targeting applications. However, its collateral cleavage of bystander RNAs limits in vivo therapeutic applications. In this study, we generated a series of
Wenxia Zhang +9 more
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A detailed protocol for RNA cleavage assay in sympathetic neurons [PDF]
STAR Protocols, 2021 Summary: This protocol illustrates the use of an in vitro assay to study the cleavage of the IMPA1 3′UTR by the endonuclease Ago2 in sympathetic neurons.
Catia Andreassi, Antonella Riccio
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RNA-Guided RNA Cleavage by a CRISPR RNA-Cas Protein Complex [PDF]
Cell, 2009 Compelling evidence indicates that the CRISPR-Cas system protects prokaryotes from viruses and other potential genome invaders. This adaptive prokaryotic immune system arises from the clustered regularly interspaced short palindromic repeats (CRISPRs) found in prokaryotic genomes, which harbor short invader-derived sequences, and the CRISPR-associated (
Brenton R Graveley +2 more
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An Ex Vivo Protocol to Assess IRE1α-Dependent RNA Cleavage Using Total RNA Isolated from Mouse Tissues [PDF]
Bio-ProtocolRegulated IRE1-dependent decay (RIDD) is a critical cellular mechanism mediated by the endoplasmic reticulum (ER) stress sensor IRE1α, which cleaves a variety of RNA targets to regulate ER homeostasis.
Mohammad Rashid +3 more
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