Results 261 to 270 of about 1,248,361 (297)

Chemical Diversity in RNA Cleavage

Science, 1999
It was quite a shock when it was first discovered that RNA can perform catalysis, sometimes every bit as efficiently as an enzyme. In his Perspective, [Westhof][1] now describes new evidence ([ Perrotta et al. ][2]) that so-called ribozymes can perform base-catalysis without the assistance of a metal ion. In an early world containing only RNA molecules,
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RNA Recognition and Cleavage by a Splicing Endonuclease

Science, 2006
The RNA splicing endonuclease cleaves two phosphodiester bonds within folded precursor RNAs during intron removal, producing the functional RNAs required for protein synthesis. Here we describe at a resolution of 2.85 angstroms the structure of a splicing endonuclease from Archaeglobus fulgidus
Song, Xue, Kate, Calvin, Hong, Li
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Cleavage of RNA by Imidazole

2004
Development of RNA cleaving agents, artificial RNases, has attracted considerable attention in the last years, because they can find important applications in biotechnology, for manipulating RNA, and in drug design. A direct approach to design of artificial RNases consists in imitation of the active centers of natural enzymes by simple organic ...
V. V. Vlassov, A. V. Vlassov
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Enzymatic Cleavage of RNA by RNA(Nobel Lecture)

Angewandte Chemie International Edition in English, 1990
exaly   +2 more sources

An assay to determine the kinetics of RNA cleavage

Analytical Biochemistry, 1985
To evaluate some synthetic catalysts that mimic ribonuclease, a quantitative assay has been developed that measures the number of phosphate diester bonds cleaved in a polymeric RNA substrate. This assay involves determining the number of 5'-oligonucleotide termini produced during the cleavage, using polyuridylic acid as the substrate. Samples withdrawn
R, Corcoran, M, Labelle, A W, Czarnik, R, Breslow   +3 more
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[Hybridase cleavage of RNA. I. Oligonucleotide probes for regiospecific RNA cleavage].

Bioorganicheskaia khimiia, 1990
New oligonucleotide probes for regiospecific cleavage of RNA molecules by hybridase (RNase H) are suggested. RNase H from E. coli is shown to site-specifically split eight phosphodiester bonds in RNA in the heteroduplex, formed by 5S rRNA and d(ACCACCGCGCT).
V G, Metelev, N F, Krynetskaia, A A, Purmal', Z A, Shabarova   +3 more
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[Hybridase cleavage of RNA. V. Complementary precision of cleavage].

Molekuliarnaia biologiia, 1992
The efficiency of the cleavage of RNA involved in perfect as well as imperfect hybrid duplexes composed of three components: (1) homogeneous RNA's or polyribonucleotides; (2) corresponding complementary synthetic oligodeoxyribonucleotides; (3) E. coli RNase H was investigated.
V G, Metelev, N F, Krynetskaia, E M, Volkov, T S, Oretskaia, Z A, Shabarova   +4 more
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trans Single‐Stranded DNA Cleavage via CRISPR/Cas14a1 Activated by Target RNA without Destruction

Angewandte Chemie - International Edition, 2021
Yi Wan, Jinghong Li
exaly  

N6‐Isopentenyladenosine in RNA Determines the Cleavage Site of Endonuclease Deoxyribozymes

Angewandte Chemie - International Edition, 2020
Claudia Hobartner, Maksim Sednev, Carina Stiller   +2 more
exaly  

Design, Synthesis, and Evaluation of Neomycin‐Imidazole Conjugates for RNA Cleavage

ChemPlusChem, 2022
Stéphane Azoulay, Maria Duca
exaly