Results 41 to 50 of about 1,834,577 (295)

stepRNA: Identification of Dicer cleavage signatures and passenger strand lengths in small RNA sequences

Frontiers in Bioinformatics, 2022
The enzyme Dicer is a component of many small RNA (sRNA) pathways involved in RNA processing for post-transcriptional regulation, anti-viral response and control of transposable elements.
Ben Murcott, Rebecca J. Pawluk, Anna V. Protasio, Ruth Y. Akinmusola, Dominika Lastik, Vicky L. Hunt   +5 more
doaj   +1 more source

A programmable pAgo nuclease with RNA target-cleavage specificity from the mesophilic bacterium Verrucomicrobia

Acta Biochimica et Biophysica Sinica, 2023
Argonaute (Ago) proteins are conserved programmable nucleases present in eukaryotes and prokaryotes and provide defense against mobile genetic elements. Almost all characterized pAgos prefer to cleave DNA targets.
Liu Qi, Chen Wanping, Zhang Yue, Hu Fengyang, Jiang Xiaoman, Wang Fei, Liu Yang, Ma Lixin   +7 more
doaj   +1 more source

Regulation of coronavirus nsp15 cleavage specificity by RNA structure

PLoS ONE, 2023
SARS-CoV-2, the etiologic agent of the COVID-19 pandemic, has had an enduring impact on global public health. However, SARS-CoV-2 is only one of multiple pathogenic human coronaviruses (CoVs) to have emerged since the turn of the century. CoVs encode for
Indraneel Salukhe, Ryan Choi, Wesley Van Voorhis, Lynn Barrett, Jennifer Hyde   +4 more
doaj   +2 more sources

Further Probing of Cu²⁺-Dependent PNAzymes Acting as Artificial RNA Restriction Enzymes

Molecules, 2019
Peptide nucleic acid (PNA)-neocuproine conjugates have been shown to efficiently catalyse the cleavage of RNA target sequences in the presence of Cu2+ ions in a site-specific manner. These artificial enzymes are designed to force the formation of a bulge
Olivia Luige, Merita Murtola, Alice Ghidini, Roger Strömberg   +3 more
doaj   +1 more source

Transcriptome wide annotation of eukaryotic RNase III reactivity and degradation signals. [PDF]

PLoS Genetics, 2015
Detection and validation of the RNA degradation signals controlling transcriptome stability are essential steps for understanding how cells regulate gene expression. Here we present complete genomic and biochemical annotations of the signals required for
Jules Gagnon, Mathieu Lavoie, Mathieu Catala, Francis Malenfant, Sherif Abou Elela   +4 more
doaj   +1 more source

RNase H1-Dependent Antisense Oligonucleotides Are Robustly Active in Directing RNA Cleavage in Both the Cytoplasm and the Nucleus

Molecular Therapy, 2017
RNase H1-dependent antisense oligonucleotides (ASOs) are active in reducing levels of both cytoplasmic mRNAs and nuclear retained RNAs. Although ASO activity in the nucleus has been well demonstrated, the cytoplasmic activity of ASOs is less clear. Using
Xue-hai Liang, Hong Sun, Josh G. Nichols, S. Crooke   +3 more
semanticscholar   +1 more source

Enzymatic cleavage of RNA by RNA

Trends in Biochemical Sciences, 1986
The discovery and characterization of the catalytic RNA subunit of the enzyme ribonuclease P ofEscherichia coli is described.
Sidney Altman, Madeline Baer, Cecilia Guerrier-Takada, Agustin Vioque   +3 more
openaire   +1 more source

Conserved functions of the trigger loop and Gre factors in RNA cleavage by bacterial RNA polymerases

Journal of Biological Chemistry, 2017
RNA cleavage by RNA polymerase (RNAP) is the central step in co-transcriptional RNA proofreading. Bacterial RNAPs were proposed to rely on the same mobile element of the active site, the trigger loop (TL), for both nucleotide addition and RNA cleavage ...
Nataliya Miropolskaya, D. Esyunina, A. Kulbachinskiy   +2 more
semanticscholar   +1 more source

The Endonucleolytic RNA Cleavage Function of nsp1 of Middle East Respiratory Syndrome Coronavirus Promotes the Production of Infectious Virus Particles in Specific Human Cell Lines

Journal of Virology, 2018
MERS-CoV represents a high public health threat. Because CoV nsp1 is a major viral virulence factor, uncovering the biological functions of MERS-CoV nsp1 could contribute to our understanding of MERS-CoV pathogenicity and spur development of medical ...
Keisuke Nakagawa, Krishna Shankara Narayanan, M. Wada, V. Popov, M. Cajimat, R. Baric, S. Makino   +6 more
semanticscholar   +1 more source

RNA cleavage products generated by antisense oligonucleotides and siRNAs are processed by the RNA surveillance machinery

Nucleic Acids Research, 2016
DNA-based antisense oligonucleotides (ASOs) elicit cleavage of the targeted RNA by the endoribonuclease RNase H1, whereas siRNAs mediate cleavage through the RNAi pathway.
W. Lima, C. L. de Hoyos, Xue-hai Liang, S. Crooke   +3 more
semanticscholar   +1 more source