Results 51 to 60 of about 1,834,577 (295)
Intracellular RNA cleavage by the hairpin ribozyme [PDF]
Nucleic Acids Research, 1998 Studies involving ribozyme-directed inactivation of targeted RNA molecules have met with mixed success, making clear the importance of methods to measure and optimize ribozyme activity within cells. The interpretation of biochemical assays for determining ribozyme activity in the cellular environment have been complicated by recent results indicating ...
A A, Seyhan, J, Amaral, J M, Burke
openaire +2 more sources
CFIm25 in Solid Tumors: Current Research Progress
Technology in Cancer Research & Treatment, 2020 Cleavage factor I m25 is a newly discovered solid tumor-related gene, however, its precise role in cancer pathogenesis has not yet been characterized.
Xiaojie Sun MD, PhD +4 more
doaj +1 more source
Probing RNA Structure by Lead Cleavage [PDF]
Current Protocols in Nucleic Acid Chemistry, 2000 AbstractLead cleavage causes a transesterification reaction that breaks the 5′,3′‐phosphodiester backbone of RNA, leaving a 2′,3′‐cyclic phosphate and a 5′‐hydroxyl. Since the efficiency of the reaction at the 2′‐hydroxyl is related to steric and chemical constraints on particular 2′‐hydroxyls embedded in the RNA, this reaction can be used to examine ...
openaire +3 more sources
Distinct roles for Argonaute proteins in small RNA-directed RNA cleavage pathways.
Genes & Development, 2004 In mammalian cells, both microRNAs (miRNAs) and small interfering RNAs (siRNAs) are thought to be loaded into the same RNA-induced silencing complex (RISC), where they guide mRNA degradation or translation silencing depending on the complementarity of ...
K. Okamura +3 more
semanticscholar +1 more source
Cleavage of Influenza RNA Using Artificial RNA‐cleaving Enzyme
The FASEB Journal, 2021 Previously, we reported that our designed artificial DNA‐cleaving enzyme which comprises our artificial DNA‐binding protein and a DNA‐cleaving domain inhibited DNA replication of human papilloma virus (HPV) in mammalian cells by cleaving its target HPV ori plasmid sequence‐specifically.
Tomoaki Mori +5 more
openaire +1 more source
FEBS Letters, EarlyView.This perspective highlights emerging insights into how the circadian transcription factor CLOCK:BMAL1 regulates chromatin architecture, cooperates with other transcription factors, and coordinates enhancer dynamics. We propose an updated framework for how circadian transcription factors operate within dynamic and multifactorial chromatin landscapes ...
Xinyu Y. Nie, Jerome S. Menet
wiley +1 more source
Cell Reports, 2014 The Cmr complex is the multisubunit effector complex of the type III-B clustered regularly interspaced short palindromic repeats (CRISPR)-Cas immune system. The Cmr complex recognizes a target RNA through base pairing with the integral CRISPR RNA (crRNA)
N. Ramia +11 more
semanticscholar +1 more source
Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation
FEBS Letters, EarlyView.Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe +3 more
wiley +1 more source
RNA and DNA Targeting by a Reconstituted Thermus thermophilus Type III-A CRISPR-Cas System. [PDF]
PLoS ONE, 2017 CRISPR-Cas (clustered regularly interspaced short palindromic repeats-CRISPR-associated) systems are RNA-guided adaptive immunity pathways used by bacteria and archaea to defend against phages and plasmids.
Tina Y Liu +2 more
doaj +1 more source
Nucleic Acids Research, 2014 In metazoans, cleavage by the endoribonuclease SMG6 is often the first degradative event in non-sense-mediated mRNA decay (NMD). However, the exact sites of SMG6 cleavage have yet to be determined for any endogenous targets, and most evidence as to the ...
Skye A. Schmidt +7 more
semanticscholar +1 more source