Viruses and the cellular RNA decay machinery. [PDF]
WIREs RNA, 2010 The ability to control cellular and viral gene expression, either globally or selectively, is central to a successful viral infection, and it is also crucial for the host to respond and eradicate pathogens.
Gaglia, Marta, Glaunsinger, Britt
core +9 more sources
In vitro RNA Cleavage Assays to Characterize IRE1-dependent RNA Decay [PDF]
Bio-Protocol, 2019 The kinase/RNase IRE1 is a key effector of the cellular response to endoplasmic reticulum stress. The RNase activity of IRE1 can be measured in cells or in the test tube.
G. Karagöz +3 more
doaj +5 more sources
Genome‐wide study of mRNA degradation and transcript elongation in Escherichia coli [PDF]
Molecular Systems Biology, 2015 An essential part of gene expression is the coordination of RNA synthesis and degradation, which occurs in the same cellular compartment in bacteria.
Huiyi Chen +3 more
doaj +3 more sources
Full-length direct RNA sequencing uncovers stress granule-dependent RNA decay upon cellular stress [PDF]
eLifeCells react to stress by triggering response pathways, leading to extensive alterations in the transcriptome to restore cellular homeostasis. The role of RNA metabolism in shaping the cellular response to stress is vital, yet the global changes in RNA ...
Showkat Ahmad Dar +9 more
doaj +2 more sources
A rapid inducible RNA decay system reveals fast mRNA decay in P-bodies [PDF]
Nature CommunicationsRNA decay is vital for regulating mRNA abundance and gene expression. Existing technologies lack the spatiotemporal precision or transcript specificity to capture the stochastic and transient decay process.
Lauren A. Blake +4 more
doaj +2 more sources
The zinc finger protein ZFP36L2 inhibits flavivirus infection via the 5′-3′ XRN1-mediated RNA decay pathway in the replication complexes [PDF]
Journal of Biomedical ScienceBackground The zinc finger protein 36-like (ZFP36L) family is a CCCH-type group consisting of RNA-binding proteins, i.e., ZFP36L1 and ZFP36L2, which regulate cellular mRNA through the RNA decay pathway. ZFP36L1 combats flavivirus infections through the 5′
Ren-Jye Lin +5 more
doaj +2 more sources
Inference of RNA decay rate from transcriptional profiling highlights the regulatory programs of Alzheimer's disease. [PDF]
, 2017 The abundance of mRNA is mainly determined by the rates of RNA transcription and decay. Here, we present a method for unbiased estimation of differential mRNA decay rate from RNA-sequencing data by modeling the kinetics of mRNA metabolism.
Alkallas, Rached +3 more
core +5 more sources
Preparation of RNA 3’ End Sequencing Libraries of Total and 4-thiouracil Labeled RNA for Simultaneous Measurement of Transcription, RNA Synthesis and Decay in S. cerevisiae [PDF]
Bio-Protocol, 2019 Cellular RNA levels are determined by the rates of RNA transcription from the gene template and subsequent RNA stability. Knowledge about both transcription and RNA decay is, therefore, necessary to interpret RNA levels and gene expression, especially ...
Manfred Schmid +2 more
doaj +2 more sources
UPF1-Mediated RNA Decay—Danse Macabre in a Cloud [PDF]
Biomolecules, 2020 Nonsense-mediated RNA decay (NMD) is the prototype example of a whole family of RNA decay pathways that unfold around a common central effector protein called UPF1.
Daria Lavysh, Gabriele Neu-Yilik
doaj +2 more sources
eLife, 2017 Nuclear RNA exosomes catalyze a range of RNA processing and decay activities that are coordinated in part by cofactors, including Mpp6, Rrp47, and the Mtr4 RNA helicase.
Elizabeth V Wasmuth +4 more
doaj +2 more sources