Results 161 to 170 of about 3,502 (177)
Some of the next articles are maybe not open access.
Dynamics of the canonical RNA degradosome components during glucose stress
Biochimie, 2021The RNA Degradosome (RNAD) is a multi-enzyme complex, which performs important functions in post-transcriptional regulation in Escherichia coli with the assistance of regulatory sRNAs and the RNA chaperone Hfq. Although the interaction of the canonical RNAD components with RNase E has been extensively studied, the dynamic nature of the interactions in ...
Marcos Emmanuel Jaso-Vera +3 more
openaire +2 more sources
Current perspectives of the Escherichia coli RNA degradosome
Biotechnology Letters, 2011Many biological processes in the cell are linked to RNA metabolism and therefore have implications for a wide range of biotechnological applications. The processing and degradation of RNA plays an important role in RNA metabolism with often the same enzymes being involved in both processes.
Adélle, Burger +2 more
openaire +2 more sources
Escherichia coli RNA Degradosome
2001Publisher Summary The Escherichia coli RNA degradosome is a nucleolytic complex containing four integral components: ribonuclease E (RNase E), polynucleotide phosphorylase (PNPase), RNA helicase B (RhlB), and enolase. This complex was discovered during efforts to purify and characterize RNase E, which is a singlestrand-specific endoribonuclease ...
A J, Carpousis +3 more
openaire +2 more sources
Analysis of the RNA degradosome complex in Vibrio angustum S14
The FEBS Journal, 2010The RNA degradosome is built on the C‐terminal half of ribonuclease E (RNase E) which shows high sequence variation, even amongst closely related species. This is intriguing given its central role in RNA processing and mRNA decay. Previously, we have identified RhlB (ATP‐dependent DEAD‐box RNA helicase)‐binding, PNPase (polynucleotide phosphorylase ...
Melissa A, Erce +2 more
openaire +2 more sources
Polyphosphate kinase is a component of the Escherichia coli RNA degradosome
Molecular Microbiology, 1997The Escherichia coli degradosome is a multienzyme complex with four major protein components: the endoribonuclease RNase E, the exoribonuclease PNPase, the RNA helicase RhlB and enolase. The first three of these proteins are known to have important functions in mRNA processing and degradation.
E, Blum +3 more
openaire +2 more sources
Analysis of the Escherichia coli RNA degradosome composition by a proteomic approach
Biochimie, 2006The RNA degradosome is a bacterial protein machine devoted to RNA degradation and processing. In Escherichia coli it is typically composed of the endoribonuclease RNase E, which also serves as a scaffold for the other components, the exoribonuclease PNPase, the RNA helicase RhlB, and enolase.
M.E. Regonesi +7 more
openaire +4 more sources
The assembly and distribution in vivo of the Escherichia coli RNA degradosome
Biochimie, 2013We report an analysis in vivo of the RNA degradosome assembly of Escherichia coli. Employing fluorescence microscopy imaging and fluorescence energy transfer (FRET) measurements, we present evidence for in vivo pairwise interactions between RNase E-PNPase (polynucleotide phosphorylase), and RNase E-Enolase.
Lilianha, Domínguez-Malfavón +4 more
openaire +2 more sources
Preparation of Escherichia coli Rne Protein and Reconstitution of RNA Degradosome
2001Publisher Summary This chapter describes methods for the purification of Rne, the key scaffold in the assembly process, and for the reconstitution of active degradosomes from individually purified enzymes. The endoribonuclease RNase E was initially described as an activity required for the penultimate step in the maturation of 5S rRNA, the processing
G A, Mackie +4 more
openaire +2 more sources
Towards Understanding Helicase and Chaperone Activities in the RNA Degradosome
2020The E. coli RNA degradosome is a complex multi-enzyme machine which is central to the post-transcriptional regulation of the cell. Some of its functions include maturing and processing sRNA, rRNA, and tRNA, as well as degrading mRNA. The key components of the RNA degradosome include the endoribonuclease RNase E, the DEAD-box RNA helicase RhlB, the ...
openaire +1 more source
A proteomic approach to the analysis of RNA degradosome composition in Escherichia coli.
Methods in enzymology, 2008The RNA degradosome is a bacterial protein machine devoted to RNA degradation and processing. In Escherichia coli, it is typically composed of the endoribonuclease RNase E, which also serves as a scaffold for the other components: the exoribonuclease PNPase, the RNA helicase RhlB, and enolase.
P. Mauri, G. Dehò
openaire +3 more sources