Results 81 to 90 of about 125,618 (325)
Expression of catalytic mutants of the mtDNA helicase Twinkle and polymerase POLG causes distinct replication stalling phenotypes. [PDF]
, 2007 The mechanism of mitochondrial DNA replication is a subject of intense debate. One model proposes a strand-asynchronous replication in which both strands of the circular genome are replicated semi-independently while the other model proposes both a ...
Goffart, S +4 more
core +1 more source
Architecture of a SARS-CoV-2 mini replication and transcription complex
Nature Communications, 2020 SARS-CoV-2 virus replication and transcription is mediated by the replication and transcription complex (RTC) that is composed of 16 non-structural proteins (nsp). Here, the authors present the cryo-EM structure of a SARS-CoV-2 mini RTC consisting of the
Liming Yan +12 more
doaj +1 more source
Human Schlafen 14 Cleavage of Short Double‐Stranded RNAs Underpins its Antiviral Activity
Advanced Science, EarlyView.SLFN14 is associated with human diseases. SLFN14 is found to cleave RNAs containing short duplexes. The cryo‐EM structures of SLFN14 and SLFN14‐hairpin RNA complex reveal that SLFN14 assembles into a ring‐like dimer; two RNase domains form an RNA‐binding groove accommodating a hairpin RNA.
Mengyun Li +8 more
wiley +1 more source
Rice SUV3 is a bidirectional helicase that binds both DNA and RNA [PDF]
, 2014 BACKGROUND: Helicases play crucial role in almost all the nucleic acid metabolism including replication, repair, recombination, transcription, translation, ribosome biogenesis and splicing and these processes regulate plant growth and development.
Mohammed Tarique +2 more
core +1 more source
Monitoring helicase activity with molecular beacons
BioTechniques, 2008 A high-throughput, fluorescence-based helicase assay using molecular beacons is described. The assay is tested using the NS3 helicase encoded by the hepatitis C virus (HCV) and is shown to accurately monitor helicase action on both DNA and RNA.
Craig A. Belon, David N. Frick
doaj +1 more source
eLife, 2021 The PcrA/UvrD helicase binds directly to RNA polymerase (RNAP) but the structural basis for this interaction and its functional significance have remained unclear.
Inigo Urrutia-Irazabal +4 more
doaj +1 more source
RNA helicases and remodeling proteins
Current Opinion in Chemical Biology, 2011 It is becoming increasingly clear that RNA molecules play a major role in all aspects of metabolism. The conformational state and stability of RNA are controlled by RNA remodeling proteins, which are ubiquitous motor proteins in the cell. Here, we review advances in our understanding of the structure and function of three major structural families of ...
openaire +3 more sources
RNA helicase A is essential for normal gastrulation [PDF]
Proceedings of the National Academy of Sciences, 1998 RNA helicase A (RHA) is the human homologue of the Drosophila maleless protein, an essential factor for the development of male flies. Recently, it was shown that RHA cooperates with the cAMP-responsive element in mediating the cAMP-dependent transcriptional activation of a number of genes.
Vera Soares +5 more
openaire +3 more sources
Advanced Science, EarlyView.Japanese encephalitis virus (JEV) employs a cap‐independent (CI) translation strategy to evade host translational shutoff. During the shut‐off of host cellular canonical translation, DEAD‐box protein 3 (DDX3) bind to JEV UTRs to establish a closed‐loop architecture, and its interaction with poly(A)‐binding protein 1 (PABP1) to form DDX3/PABP1/eIF4G ...
Chenxi Li +13 more
wiley +1 more source
Degradation of Hypomodified tRNA\u3csub\u3ei\u3c/sub\u3e\u3csup\u3eMet\u3c/sup\u3e in vivo Involves RNA-dependent ATPase Activity of the DExH Helicase Mtr4p [PDF]
, 2008 Effective turnover of many incorrectly processed RNAs in yeast, including hypomodified tRNAi Met, requires the TRAMP complex, which appends a short poly(A) tail to RNA designated for decay. The poly(A) tail stimulates degradation by the exosome.
Anderson, James T. +3 more
core +1 more source