Results 121 to 130 of about 753,590 (330)

A mutate-and-map protocol for inferring base pairs in structured RNA

, 2013
Chemical mapping is a widespread technique for structural analysis of nucleic acids in which a molecule's reactivity to different probes is quantified at single-nucleotide resolution and used to constrain structural modeling.
CS Chow, D Moazed, DH Mathews, EE Regulski, JB Lucks, JG Underwood, JP Staley, KA Wilkinson, M Kertesz, M Mandal, N Kulshina, NB Leontis, P Cordero, P Cordero, P Rocca-Serra, P Tijerina, PJ Lukavsky, R Rydzanicz, RC Spitale, RF Gesteland, S Mitra, S Washietl, S Yoon, SA Mortimer, SE Wells, SM Vasa, SW Brostoff, VD Sood, W Kladwang, W Kladwang, W Kladwang, W Kladwang, Y Rhee   +32 more
core   +1 more source

HiTRACE-Web: an online tool for robust analysis of high-throughput capillary electrophoresis [PDF]

, 2013
To facilitate the analysis of large-scale high-throughput capillary electrophoresis data, we previously proposed a suite of efficient analysis software named HiTRACE (High Throughput Robust Analysis of Capillary Electrophoresis).
Cordero, Pablo, Das, Rhiju, Kim, Hanjoo, Yoon, Sungroh   +3 more
core   +2 more sources

Clinical applications of next‐generation sequencing‐based ctDNA analyses in breast cancer: defining treatment targets and dynamic changes during disease progression

Molecular Oncology, EarlyView.
Circulating tumor DNA (ctDNA) offers a possibility for different applications in early and late stage breast cancer management. In early breast cancer tumor informed approaches are increasingly used for detecting molecular residual disease (MRD) and early recurrence. In advanced stage, ctDNA provides a possibility for monitoring disease progression and
Eva Valentina Klocker, Samantha Hasenleithner, Rupert Bartsch, Simon P. Gampenrieder, Daniel Egle, Christian F. Singer, Gabriel Rinnerthaler, Michael Hubalek, Katja Schmitz, Zsuzsanna Bago‐Horvath, Andreas Petzer, Sonja Heibl, Ellen Heitzer, Marija Balic, Michael Gnant   +14 more
wiley   +1 more source

Extension of the crRNA enhances Cpf1 gene editing in vitro and in vivo. [PDF]

, 2018
Engineering of the Cpf1 crRNA has the potential to enhance its gene editing efficiency and non-viral delivery to cells. Here, we demonstrate that extending the length of its crRNA at the 5 end can enhance the gene editing efficiency of Cpf1 both in cells
Chong, Anthony, Chu, Hunghao, Fellmann, Christof, Jiang, Fuguo, Lee, Kunwoo, Liu, Hui, Mackley, Vanessa, Murthy, Niren, Park, Hyo, Rao, Anirudh, Wu, Joann   +10 more
core   +2 more sources

Regulatory role and mechanism of m6A RNA modification in human metabolic diseases

Molecular Therapy: Oncolytics, 2021
Metabolic diseases caused by disorders in amino acids, glucose, lipid metabolism, and other metabolic risk factors show high incidences in young people, and current treatments are ineffective.
Yuliang Zhang, Wenjie Chen, Xiwang Zheng, Yujia Guo, Jimin Cao, Yu Zhang, Shuxin Wen, Wei Gao, Yongyan Wu   +8 more
doaj  

Circulating tumor cells in metastatic breast cancer patients treated with immune checkpoint inhibitors – a biomarker analysis of the ALICE and ICON trials

Molecular Oncology, EarlyView.
In this explorative biomarker analysis, we assessed serial sampling of circulating tumor cells (CTCs) with CellSearch in two randomized trials testing immune checkpoint inhibitors (ICIs) in metastatic breast cancer. Our data demonstrate a prognostic potential of CTCs, most apparent 4 weeks into ICI therapy.
Nikolai Kragøe Andresen, Andreas Hagen Røssevold, Elin Borgen, Cecilie Bendigtsen Schirmer, Bjørnar Gilje, Øystein Garred, Jon Lømo, Marius Stensland, Oddmund Nordgård, Ragnhild Sørum Falk, Randi R. Mathiesen, Hege G. Russnes, Jon Amund Kyte, Bjørn Naume   +13 more
wiley   +1 more source

Syntheses of 4′-thioribonucleosides and thermodynamic stability and crystal structure of RNA oligomers with incorporated 4′-thiocytosine [PDF]

, 2017
A facile synthetic route for the 4′-thioribonucleoside building block 4′SN (N = U, C, A and G) with the ribose O4′ replaced by sulfur is presented. Conversion of l-lyxose to 1,5-di-O-acetyl-2,3-di-O-benzoyl-4-thio-d-ribofuranose was achieved via an ...
Berger, Imre, Egli, Martin, Haeberli, Peter, Pallan, Pradeep S.   +3 more
core  

RNAME: A comprehensive database of RNA modification enzymes

Computational and Structural Biotechnology Journal, 2022
The dynamic RNA modifications were orchestrated by a series of enzymes, namely “writer”, “reader” and “eraser”, which can install, recognize and remove the modifications, respectively.
Fulei Nie, Qiang Tang, Ying Liu, Huaizhi Qin, Shilong Liu, Meng Wu, Pengmian Feng, Wei Chen   +7 more
doaj  

DNA N6-Methyladenosine modification role in transmitted variations from genomic DNA to RNA in Herrania umbratica

BMC Genomics, 2019
Background DNA methylation is an important epigenetic modification. Recently the developed single-molecule real-time (SMRT) sequencing technology provided an efficient way to detect DNA N6-methyladenine (6mA) modification that played an important role in
Mei-Wei Luan, Wei Chen, Jian-Feng Xing, Chuan-Le Xiao, Ying Chen, Shang-Qian Xie   +5 more
doaj   +1 more source

Circulating tumor cells: advancing personalized therapy in small cell lung cancer patients

Molecular Oncology, EarlyView.
Small cell lung cancer (SCLC) is an aggressive form of lung cancer that spreads rapidly to secondary sites such as the brain and liver. Cancer cells circulating in the blood, “circulating tumor cells” (CTCs), have demonstrated prognostic value in SCLC, and evaluating biomarkers on CTCs could guide treatment decisions such as for PARP inhibitors ...
Prajwol Shrestha, Steven Kao, Veronica K. Cheung, Wendy A. Cooper, Nico van Zandwijk, John E. J. Rasko, Dannel Yeo   +6 more
wiley   +1 more source