Human Retrovirus Genomic RNA Packaging [PDF]
Viruses, 2022 Two non-covalently linked copies of the retrovirus genome are specifically recruited to the site of virus particle assembly and packaged into released particles.
Heather M. Hanson +3 more
doaj +3 more sources
From Cells to Virus Particles: Quantitative Methods to Monitor RNA Packaging [PDF]
Viruses, 2016 In cells, positive strand RNA viruses, such as Retroviridae, must selectively recognize their full-length RNA genome among abundant cellular RNAs to assemble and release particles.
Mireia Ferrer +4 more
doaj +3 more sources
Nucleocapsid protein-dependent assembly of the RNA packaging signal of Middle East respiratory syndrome coronavirus [PDF]
Journal of Biomedical Science, 2018 Background Middle East respiratory syndrome coronavirus (MERS-CoV) consists of a positive-sense, single-stranded RNA genome and four structural proteins: the spike, envelope, membrane, and nucleocapsid protein. The assembly of the viral genome into virus
Wei-Chen Hsin +6 more
doaj +3 more sources
Identification of a 1.4-kb-Long Sequence Located in the nsp12 and nsp13 Coding Regions of SARS-CoV-2 Genomic RNA That Mediates Efficient Viral RNA Packaging. [PDF]
J Virol, 2023 Elucidating the mechanism of SARS-CoV-2 RNA packaging into virus particles is important for the rational design of antiviral drugs that inhibit this vital step in the replication cycle of CoVs.
Terasaki K, Narayanan K, Makino S.
europepmc +2 more sources
Epitranscriptomic regulation of HIV-1 full-length RNA packaging. [PDF]
Nucleic Acids Res, 2022 During retroviral replication, the full-length RNA serves both as mRNA and genomic RNA. However, the mechanisms by which the HIV-1 Gag protein selects the two RNA molecules that will be packaged into nascent virions remain poorly understood.
Pereira-Montecinos C +12 more
europepmc +2 more sources
RNA Packaging in the Cystovirus Bacteriophages: Dynamic Interactions during Capsid Maturation. [PDF]
Int J Mol Sci, 2022 The bacteriophage family Cystoviridae consists of a single genus, Cystovirus, that is lipid-containing with three double-stranded RNA (ds-RNA) genome segments. With regard to the segmented dsRNA genome, they resemble the family Reoviridae. Therefore, the
Gottlieb P, Alimova A.
europepmc +2 more sources
RNA packaging into extracellular vesicles: An orchestra of RNA-binding proteins? [PDF]
J Extracell Vesicles, 2020 Extracellular vesicles (EVs) are heterogeneous membranous particles released from the cells through different biogenetic and secretory mechanisms. We now conceive EVs as shuttles mediating cellular communication, carrying a variety of molecules resulting
Fabbiano F +5 more
europepmc +2 more sources
Identification of the initial nucleocapsid recognition element in the HIV-1 RNA packaging signal. [PDF]
Proc Natl Acad Sci U S A, 2020 Significance Understanding the molecular determinants of retroviral genome packaging is important for drug discovery and development of vectors for gene delivery.
Ding P +16 more
europepmc +2 more sources
mBio, 2021 Human immunodeficiency virus type 1 (HIV-1) Gag selects and packages the HIV RNA genome during virus assembly. However, HIV-1 RNA constitutes only a small fraction of the cellular RNA.
Alice Duchon +9 more
doaj +2 more sources
HIV-1 Gag protein with or without p6 specifically dimerizes on the viral RNA packaging signal. [PDF]
J Biol Chem, 2020 The HIV-1 Gag protein is responsible for genomic RNA (gRNA) packaging and immature viral particle assembly. Although the presence of gRNA in virions is required for viral infectivity, in its absence, Gag can assemble around cellular RNAs and form ...
Sarni S +7 more
europepmc +2 more sources