Results 21 to 30 of about 104,592 (252)
Specificity of retroviral RNA packaging [PDF]
Journal of Virology, 1991 Encapsidation of retroviral RNA has been shown to be dependent on specific cis-acting signals, in particular, the packaging region (psi) located near the 5' end of the retroviral genome. In this report, we show that a 683-base avian extended packaging sequence (psi+) derived from Rous sarcoma virus will direct packaging of heterologous hygromycin mRNA ...
R, Aronoff, M, Linial
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Packaging signal of influenza A virus
Virology Journal, 2021 Influenza A virus (IAV) contains a genome with eight single-stranded, negative-sense RNA segments that encode 17 proteins. During its assembly, all eight separate viral RNA (vRNA) segments are incorporated into virions in a selective manner.
Xiuli Li +4 more
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RNA Origami: Packaging a Segmented Genome in Orbivirus Assembly and Replication
Viruses, 2021 Understanding how viruses with multi-segmented genomes incorporate one copy of each segment into their capsids remains an intriguing question. Here, we review our recent progress and describe the advancements made in understanding the genome packaging ...
Po-Yu Sung, Polly Roy
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Murine coronavirus packaging signal confers packaging to nonviral RNA [PDF]
Journal of Virology, 1997 Studies of defective interfering (DI) RNAs of the murine coronavirus mouse hepatitis virus (MHV) suggest that a 69-nucleotide-long packaging signal is necessary for MHV genomic RNA packaging into MHV particles. In this study we showed that when RNA transcripts that consisted of a non-MHV sequence and the packaging signal were expressed in MHV-infected ...
K, Woo +4 more
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Frontiers in Cellular and Infection Microbiology, 2023 Rift Valley fever virus (RVFV), a bunyavirus, has a single-stranded, negative-sense tri-segmented RNA genome, consisting of L, M and S RNAs. An infectious virion carries two envelope glycoproteins, Gn and Gc, along with ribonucleoprotein complexes ...
Breanna Tercero +8 more
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Cooperative Mechanism of RNA Packaging Motor [PDF]
Journal of Biological Chemistry, 2005 P4 is a hexameric ATPase that serves as the RNA packaging motor in double-stranded RNA bacteriophages from the Cystoviridae family. P4 shares sequence and structural similarities with hexameric helicases. A structure-based mechanism for mechano-chemical coupling has recently been proposed for P4 from bacteriophage phi12.
Jirí, Lísal, Roman, Tuma
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Cell biology of retroviral RNA packaging [PDF]
RNA Biology, 2011 Generation of infectious retroviral particles rely on the targeting of all structural components to the correct cellular sites at the correct time. Gag, the main structural protein, orchestrates the assembly process and the mechanisms that trigger its targeting to assembly sites are well described. Gag is also responsible for the packaging of the viral
Nolwenn, Jouvenet +3 more
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Packaging of Genomic RNA in Positive-Sense Single-Stranded RNA Viruses: A Complex Story
Viruses, 2019 The packaging of genomic RNA in positive-sense single-stranded RNA viruses is a key part of the viral infectious cycle, yet this step is not fully understood. Unlike double-stranded DNA and RNA viruses, this process is coupled with nucleocapsid assembly.
Mauricio Comas-Garcia
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Development of a Novel Competitive qRT-PCR Assay to Measure Relative Lentiviral Packaging Efficiency
Molecular Therapy: Methods & Clinical Development, 2020 Third-generation HIV-1-derived lentiviral vectors are successfully used as therapeutic agents in various clinical applications. To further promote their use, we attempted to enhance vector infectivity by targeting the dimerization and packaging ...
Eirini Vamva +3 more
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Microbiology Spectrum, 2022 HIV-1 must package its RNA genome to generate infectious viruses. Recent studies have revealed that during genome packaging, HIV-1 not only excludes cellular mRNAs, but also distinguishes among full-length viral RNAs. Using NL4-3 and MAL molecular clones,
Jonathan M. O. Rawson +5 more
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