Reconstitution of selective HIV-1 RNA packaging in vitro by membrane-bound Gag assemblies. [PDF]
Elife, 2016 HIV-1 Gag selects and packages a dimeric, unspliced viral RNA in the context of a large excess of cytosolic human RNAs. As Gag assembles on the plasma membrane, the HIV-1 genome is enriched relative to cellular RNAs by an unknown mechanism.
Carlson LA +4 more
europepmc +2 more sources
Cell biology of retroviral RNA packaging [PDF]
RNA Biology, 2011 Generation of infectious retroviral particles rely on the targeting of all structural components to the correct cellular sites at the correct time. Gag, the main structural protein, orchestrates the assembly process and the mechanisms that trigger its ...
N. Jouvenet +3 more
semanticscholar +4 more sources
Identification of unique reciprocal and non reciprocal cross packaging relationships between HIV-1, HIV-2 and SIV reveals an efficient SIV/HIV-2 lentiviral vector system with highly favourable features for in vivo testing and clinical usage [PDF]
Retrovirology, 2005 Background Lentiviral vectors have shown immense promise as vehicles for gene delivery to non-dividing cells particularly to cells of the central nervous system (CNS).
Caldwell Maeve +6 more
doaj +2 more sources
RNA structure. Structure of the HIV-1 RNA packaging signal. [PDF]
Science, 2015 Keane SC +16 more
europepmc +2 more sources
Cooperation of an RNA Packaging Signal and a Viral Envelope Protein in Coronavirus RNA Packaging [PDF]
Journal of Virology, 2001 ABSTRACT Murine coronavirus mouse hepatitis virus (MHV) produces a genome-length mRNA, mRNA 1, and six or seven species of subgenomic mRNAs in infected cells. Among these mRNAs, only mRNA 1 is efficiently packaged into MHV particles. MHV N protein binds to all MHV mRNAs, whereas envelope M protein interacts only with mRNA 1. This
Krishna Narayanan, Shinji Makino
openalex +4 more sources
The 5′-end segment-specific noncoding region of influenza A virus regulates both competitive multi-segment RNA transcription and selective genome packaging during infection [PDF]
Journal of VirologyThe 3′ and 5′ termini of the eight RNA segments of the influenza A virus (IAV) genome contain segment-specific or subtype-specific (in HA and NA segments) noncoding regions (ssNCRs), located between the highly conserved terminal promoter sequences and ...
Zining Liu +4 more
doaj +2 more sources
The heart of the HIV RNA packaging signal? [PDF]
Proc Natl Acad Sci U S A, 2020 Mechanisms ensuring faithful reproduction are enforced for viruses, as for all other organisms, by natural selection. As a virus particle is a package containing the viral genome (RNA or DNA, as the case may be) for replication and transmission to the next generation, it is essential that the genome be packaged into the assembling virus particle with ...
Rein A.
europepmc +4 more sources
The curious case of genome packaging and assembly in RNA viruses infecting plants
Frontiers in Genetics, 2023 Genome packaging is the crucial step for maturation of plant viruses containing an RNA genome. Viruses exhibit a remarkable degree of packaging specificity, despite the probability of co-packaging cellular RNAs.
Tushar Ranjan +9 more
doaj +1 more source
Packaging in a yeast double-stranded RNA virus [PDF]
Journal of Virology, 1995 The yeast virus ScV-L1 has only two genes, cap and pol, which encode the capsid polypeptide and the viral polymerase, respectively. The second gene is translated only as a cap-pol fusion protein. This fusion protein is responsible for recognition of a specific small stem and loop region of the viral plus strands, of 19 to 31 bases in length, ensuring ...
Wensheng Yao +2 more
openalex +5 more sources
HIV-1 Packaging Visualised by In-Gel SHAPE
Viruses, 2021 HIV-1 packages two copies of its gRNA into virions via an interaction with the viral structural protein Gag. Both copies and their native RNA structure are essential for virion infectivity.
Aaron R. D’Souza +8 more
doaj +1 more source