Results 1 to 10 of about 2,226,379 (341)
The architecture of RNA polymerase fidelity [PDF]
BMC Biology, 2010 The basis for transcriptional fidelity by RNA polymerase is not understood, but the 'trigger loop', a conserved structural element that is rearranged in the presence of correct substrate nucleotides, is thought to be critical.
Kaplan Craig D
doaj +4 more sources
Analysis of Template Variations on RNA Synthesis by Respiratory Syncytial Virus Polymerase
Viruses, 2022 Respiratory syncytial virus (RSV) is a significant threat to infants and elderly individuals globally. Currently, there are no effective therapies or treatments for RSV infection because of an insufficient understanding of the RSV viral machinery.
Dongdong Cao +8 more
doaj +1 more source
Promoter-proximal pausing of RNA polymerase II: a nexus of gene regulation
Genes & Development, 2019 In this review, Core et al. discuss the recent advances in our understanding of the early steps in Pol II transcription, highlighting the events and factors involved in the establishment and release of paused Pol II.
Leighton J. Core, K. Adelman
semanticscholar +1 more source
RNA-dependent RNA Polymerase Assay for Hepatitis E Virus
Bio-Protocol, 2017 RNA-dependent RNA polymerase (RdRp) is essential for the replication of viral RNA for RNA viruses. It synthesizes the complementary strand of viral genomic RNA, which is used subsequently as a template to generate more copies of viral genome.
Vidya Nair +3 more
doaj +1 more source
Computational Study of SARS-CoV-2 RNA Dependent RNA Polymerase Allosteric Site Inhibition
Molecules, 2021 The COVID-19 pandemic has caused millions of fatalities since 2019. Despite the availability of vaccines for this disease, new strains are causing rapid ailment and are a continuous threat to vaccine efficacy.
Shah Faisal +6 more
doaj +1 more source
Definitions, 2020 RNA polymerases comprise a group of enzymes that catalyze extension, one nucleotide at a time, of the 3 prime-end of an RNA strand along a complementary DNA template.
semanticscholar +1 more source
Structure of human RNA polymerase III
Nature Communications, 2020 The eukaryotic RNA Polymerase III transcribes tRNAs, some ribosomal and spliceosomal RNAs. Here, the authors resolve a cryo-EM structure of human RNA Polymerase III in its apo form and complemented it with crystal structures and SAXS analysis of RPC5 ...
Ewan Phillip Ramsay +10 more
doaj +1 more source
Proceedings of the National Academy of Sciences of the United States of America, 1985 The RNA polymerase gene of bacteriophage T7 has been cloned into the plasmid pBR322 under the inducible control of the lambda PL promoter. After induction, T7 RNA polymerase constitutes 20% of the soluble protein of Escherichia coli, a 200-fold increase ...
S. Tabor, C. Richardson
semanticscholar +1 more source
NeoRdRp2 with improved seed data, annotations, and scoring
Frontiers in VirologyRNA-dependent RNA polymerase (RdRp) is a marker gene for RNA viruses; thus, it is widely used to identify RNA viruses from metatranscriptome data. However, because of the high diversity of RdRp domains, it remains difficult to identify RNA viruses using ...
Shoichi Sakaguchi +4 more
doaj +1 more source
Imaging RNA polymerase III transcription using a photostable RNA-fluorophore complex
Nature Chemical Biology, 2017 Quantitative measurement of transcription rates in live cells is important for revealing mechanisms of transcriptional regulation. This is particularly challenging for measuring the activity of RNA polymerase III (Pol III), which transcribes growth ...
Wenjiao Song +6 more
semanticscholar +1 more source