Results 131 to 140 of about 2,625,316 (382)

In vitro RNA-dependent RNA Polymerase Assay Using Arabidopsis RDR6

Bio-Protocol, 2018
RNA-dependent RNA polymerases (RdRPs) in eukaryotes convert single-stranded RNAs into double-stranded RNAs, thereby amplifying small interfering RNAs that play crucial roles in the regulation of development, maintenance of genome integrity and antiviral ...
Kyungmin Baeg, Yukihide Tomari, Hiro-oki Iwakawa   +2 more
doaj   +1 more source

Transcription-associated breaks in Xeroderma Pigmentosum group D cells from patients with combined features of Xeroderma Pigmentosum and Cockayne Syndrome [PDF]

, 2005
Defects in the XPD gene can result in several clinical phenotypes, including xeroderma pigmentosum (XP), trichothiodystrophy, and, less frequently, the combined phenotype of XP and Cockayne syndrome (XP-D/CS).
Andressoo, Jaan-Olle, Botta, Elena, Fousteri, Maria I., Fujimoto, Mitsuo, Harries, Lorna W., Jaspers, Nicolaas G. J., Lehmann, Alan R., McDaniel, Lisa D., Mitchell, Jay, Mullenders, Leon H., Stefanini, Miria, Theron, Therina, Volker, Marcel   +12 more
core   +3 more sources

Transcriptome‐wide analysis of circRNA and RBP profiles and their molecular relevance for GBM

Molecular Oncology, EarlyView.
CircRNAs are differentially expressed in glioblastoma primary tumors and might serve as therapeutic targets and diagnostic markers. The investigation of circRNA and RNA‐binding proteins (RBPs) interactions shows that distinct RBPs play a role in circRNA biogenesis and function.
Julia Latowska‐Łysiak, Żaneta Zarębska, Marcin P. Sajek, Adriana Grabowska, Alessia Buratin, Paweł Głodowicz, Julia O. Misiorek, Konrad Kuczyński, Stefania Bortoluzzi, Marek Żywicki, Jan G. Kosiński, Agnieszka Rybak‐Wolf, Rafał Piestrzeniewicz, Anna M. Barciszewska, Katarzyna Rolle   +14 more
wiley   +1 more source

Dynamics of Molecular Motors and Polymer Translocation with Sequence Heterogeneity [PDF]

, 2004
The effect of sequence heterogeneity on polynucleotide translocation across a pore and on simple models of molecular motors such as helicases, DNA polymerase/exonuclease and RNA polymerase is studied in detail.
Kafri, Yariv, Lubensky, David K., Nelson, David R.   +2 more
core   +2 more sources

Targeting the MDM2‐MDM4 interaction interface reveals an otherwise therapeutically active wild‐type p53 in colorectal cancer

Molecular Oncology, EarlyView.
This study investigates an alternative approach to reactivating the oncosuppressor p53 in cancer. A short peptide targeting the association of the two p53 inhibitors, MDM2 and MDM4, induces an otherwise therapeutically active p53 with unique features that promote cell death and potentially reduce toxicity towards proliferating nontumor cells.
Sonia Valentini, Giada Mele, Marika Attili, Maria Rita Assenza, Fulvio Saccoccia, Francesca Sardina, Cinzia Rinaldo, Roberto Massari, Nicola Tirelli, Alfredo Pontecorvi, Fabiola Moretti   +10 more
wiley   +1 more source

Molecular structures of unbound and transcribing RNA polymerase III

Nature, 2015
Transcription of genes encoding small structured RNAs such as transfer RNAs, spliceosomal U6 small nuclear RNA and ribosomal 5S RNA is carried out by RNA polymerase III (Pol III), the largest yet structurally least characterized eukaryotic RNA polymerase.
N. Hoffmann, A. Jakobi, M. Moreno-Morcillo, S. Glatt, J. Kosiński, W. Hagen, C. Sachse, C. Müller   +7 more
semanticscholar   +1 more source

Thermal proteome profiling and proteome analysis using high‐definition mass spectrometry demonstrate modulation of cholesterol biosynthesis by next‐generation galeterone analog VNPP433‐3β in castration‐resistant prostate cancer

Molecular Oncology, EarlyView.
Elevated level of cholesterol is positively correlated to prostate cancer development and disease severity. Cholesterol‐lowering drugs, such as statins, are demonstrated to inhibit prostate cancer. VNPP433‐3β interrupts multiple signaling and metabolic pathways, including cholesterol biosynthesis, AR‐mediated transcription of several oncogenes, mRNA 5′
Retheesh S. Thankan, Elizabeth Thomas, Mehari M. Weldemariam, Puranik Purushottamachar, Weiliang Huang, Maureen A. Kane, Yuji Zhang, Nicholas Ambulos, Bi‐Dar Wang, David Weber, Vincent C. O. Njar   +10 more
wiley   +1 more source

A Superresolution Census of RNA Polymerase [PDF]

Biophysical Journal, 2013
To read out information from its genome, a cell needs the appropriate molecular machinery, RNA polymerases (RNAPs), ribosomes, and associated factors. Thus, the quantitative level of gene expression is dependent on the availability of that machinery, which in turn depends on the external conditions (1).
openaire   +4 more sources

Reciprocating RNA Polymerase batters through roadblocks

Nature Communications
RNA polymerases must transit through protein roadblocks to produce full-length transcripts. Here we report real-time measurements of Escherichia coli RNA polymerase passing through different barriers. As intuitively expected, assisting forces facilitated,
Jin Qian, Allison Cartee, Wenxuan Xu, Yan Yan, Bing Wang, Irina Artsimovitch, David Dunlap, Laura Finzi   +7 more
doaj   +1 more source

The RNA Polymerase II Core Promoter in Drosophila. [PDF]

, 2019
Transcription by RNA polymerase II initiates at the core promoter, which is sometimes referred to as the "gateway to transcription." Here, we describe the properties of the RNA polymerase II core promoter in Drosophila The core promoter is at a strategic
Kadonaga, James T, Kassavetis, George A, Vo Ngoc, Long   +2 more
core   +1 more source