Results 311 to 320 of about 1,002,934 (332)

RNA Polymerase I-Mediated Expression of Influenza Viral RNA Molecules

Virology, 1994
RNA polymerase I transcription has been used for expression of influenza vRNA molecules, with influenza hemagglutinin or other cDNAs precisely inserted between mouse rDNA promoter and terminator sequences. In in vitro studies generation of HA vRNA transcripts in high rates and correct formation of their 5' ends as well as their 3' ends has been ...
G, Neumann, A, Zobel, G, Hobom
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A structural perspective on RNA polymerase I and RNA polymerase III transcription machineries

Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, 2013
RNA polymerase I and III are responsible for the bulk of nuclear transcription in actively growing cells and their activity impacts the cellular biosynthetic capacity. As a consequence, RNA polymerase I and III deregulation has been directly linked to cancer development.
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Phosphorylation of RNA polymerases: specific association of protein kinase NIl with RNA polymerase I

Philosophical Transactions of the Royal Society of London. B, Biological Sciences, 1983
The activities of the three DNA-dependent RNA polymerases from a rapidly growing rat tumour, Morris hepatoma 3924 A, and from rat liver were examined. The activity of RNA polymerase I was higher in the tumour than in the liver. The enhanced capacity for RNA synthesis was a result of a higher concentration of polymerase I in the tumour as ...
K M, Rose, D A, Stetler, S T, Jacob
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Mechanism of RNA Polymerase I Transcription

2004
Publisher Summary This chapter presents an overview of the RNA polymerase I (Pol I) transcription system, with particular emphasis on the latest findings on the mechanisms that regulate it. Comprehensive reviews on yeast and vertebrate RNA polymerase is also presented.
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Biochemical characterization of Mycobacterial RNA polymerases

Journal of Bacteriology
ABSTRACT Tuberculosis is caused by the bacterium Mycobacterium tuberculosis (Mtb). While eukaryotic species employ several specialized RNA polymerases (Pols) to fulfill the RNA synthesis requirements of the cell, bacterial species use a single RNA polymerase (RNAP).
Stephanie L. Cooper, Ryan M. Requijo, Aaron L. Lucius, David A. Schneider   +3 more
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Molecular Modeling of RNA Polymerase II Mutations onto DNA Polymerase I

Journal of Molecular Biology, 1994
Genetic and molecular analysis in Drosophila melanogaster identifies eight suppressor mutations in the second largest subunit of RNA polymerase II. The suppressor mutations fall into two classes: five are strong, result from the same serine to cysteine amino acid residue substitution and rescue one conditional lethal allele in the largest subunit of ...
W J, Kim, L P, Burke, M A, Mortin
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E. gracilis RNA polymerase I: A zinc metalloenzyme

Biochemical and Biophysical Research Communications, 1977
Abstract E. gracilis DNA dependent RNA polymerase I has been purified to homogeneity. α-amanitin, over the concentration range 0.05 to 200 μg/ml, does not affect its activity, consistent with its being classified as an RNA polymerase I. Based on a molecular weight of 624,000 daltons the enzyme contains 2.2 g atom of Zn but no Mn, Cu, Fe, as ...
K H, Falchuk, L, Ulpino, B, Mazus, B L, Vallee   +3 more
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RNA polymerases I and III, non-coding RNAs and cancer

Trends in Genetics, 2008
Oncogenically transformed cells overexpress the non-coding RNAs, such as pre-ribosomal RNA (rRNA) and transfer RNA (tRNA), which are produced by RNA polymerases (Pols) I and III. Recent results indicate that levels of pre-rRNA have prognostic value and that a tRNA has oncogenic potential.
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The RNA Polymerase I Transcription Machinery

2011
In this chapter, we review transcription of mammalian rRNA genes by RNA polymerase I (Pol I), a process that integrates information from cellular signaling cascades to regulate ribosome production. Although the emerging picture of Pol I transcriptional regulation reveals an unexpected level of complexity, we are beginning to understand the multiple ...
Renate Voit, Ingrid Grummt
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Purification of Active RNA Polymerase I from Yeast

2015
Eukaryotic cells employ at least three nuclear, DNA-dependent RNA polymerase systems for the synthesis of cellular RNA. RNA polymerases I, II, and III primarily produce rRNA, mRNA, and tRNA, respectively. In a rapidly growing cell, most RNA synthesis is devoted to production of the translation machinery, with rRNA synthesis by RNA polymerase I ...
Francis Dean, Appling, David Alan, Schneider   +1 more
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