Results 31 to 40 of about 616,337 (352)
Rapid, tunable, and multiplexed detection of RNA using convective array PCR
Communications Biology, 2023 Detection of RNA targets is typically achieved through RT-qPCR or RNAseq. RT-qPCR is rapid but limited in number and complexity of targets detected, while RNAseq is high-throughput but takes multiple days.
Andrew T. Sullivan +9 more
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SnapShot: RNA Structure Probing Technologies
Cell, 2018 Chemical probing coupled to high-throughput sequencing offers a flexible approach to uncover many aspects of RNA structure relevant to its cellular function. With a wide variety of chemical probes available that each report on different features of RNA molecules, a broad toolkit exists for investigating in vivo and in vitro RNA structure and ...
Paul D, Carlson +4 more
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Direct detection of Plasmodium female gametocyte-specific transcript s25 mRNA in blood by CLIP-PCR [PDF]
Jichu yixue yu linchuang, 2022 Objective To establish a molecular detection method for the Plasmodium female gametocyte. Methods Probes for capture and ligation probe-PCR (CLIP-PCR) were specific designed based on Plasmodium female gametocyte special RNA targets, which were the ...
YANG Jun-yuan, PIAN Hong-ru, YANG Ming-zhu, ZHENG Zhi
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Fluorescent labeling of plasmid DNA and mRNA : gains and losses of current labeling strategies [PDF]
, 2016 Live-cell imaging has provided the life sciences with insights into the cell biology and dynamics. Fluorescent labeling of target molecules proves to be indispensable in this regard. In this Review, we focus on the current fluorescent labeling strategies
Rombouts, Koen +2 more
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Current Opinion in Structural Biology, 2019 RNA structure underpins many essential functions in biology. New chemical reagents and techniques for probing RNA structure in living cells have emerged in recent years. High-throughput, genome-wide techniques such as Structure-seq2 and DMS-MaPseq exploit nucleobase modification by dimethylsulfate (DMS) to obtain complete structuromes, and are ...
David, Mitchell +2 more
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Probe Generation Directly from Small Numbers of Cells for DNA Microarray Studies
BioTechniques, 2003 Recently, we described a technique that allows us to prepare probes for expression profiling from 0.5–1 μg RNA without template or signal amplification.
C.C. Xiang +6 more
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In vitro identification and in silico utilization of interspecies sequence similarities using GeneChip(® )technology [PDF]
, 2005 BACKGROUND: Genomic approaches in large animal models (canine, ovine etc) are challenging due to insufficient genomic information for these species and the lack of availability of corresponding microarray platforms. To address this problem, we speculated
Garcia, Joe GN +5 more
core +3 more sources
Metabolic RNA labeling for probing RNA dynamics in bacteria [PDF]
Nucleic Acids Research, 2020 Abstract Metabolic labeling of RNAs with noncanonical nucleosides that are chemically active, followed by chemoselective conjugation with imaging probes or enrichment tags, has emerged as a powerful method for studying RNA transcription and degradation in eukaryotes.
Liying Meng +5 more
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Development of a Chemiluminescence-Based Ribonuclease Protection Assay
BioTechniques, 2002 The ribonuclease protection assay (RPA) is a widely used method for the detection and quantification of specific mRNA transcripts in a complex mixture of total RNA or mRNA molecules. While exhibiting many advantages over other RNA detection methods, RPAs
Christopher Rosenau +2 more
doaj +1 more source
Nanoribbon-Based Electronic Detection of a Glioma-Associated Circular miRNA
Biosensors, 2021 Nanoribbon chips, based on “silicon-on-insulator” structures (SOI-NR chips), have been fabricated. These SOI-NR chips, whose surface was sensitized with covalently immobilized oligonucleotide molecular probes (oDNA probes), have been employed for the ...
Yuri D. Ivanov +18 more
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