Selective Oligonucleotide and MRNA Pull-Down with Shielded Covalent Probes [PDF]
, 2013 Shielded covalent (SC) probes combine programmable base pairing, molecular conformation change, and activatable covalent crosslinking to achieve selective and durable capture of nucleic acid targets, including efficient discrimination of SNPs.
Pierce, Niles A., Vieregg, Jeffrey R.
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In vitro identification and in silico utilization of interspecies sequence similarities using GeneChip(® )technology [PDF]
, 2005 BACKGROUND: Genomic approaches in large animal models (canine, ovine etc) are challenging due to insufficient genomic information for these species and the lack of availability of corresponding microarray platforms. To address this problem, we speculated
Garcia, Joe GN +5 more
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Noncoder : a web interface for exon array-based detection of long non-coding RNAs [PDF]
, 2012 Due to recent technical developments, a high number of long non-coding RNAs (lncRNAs) have been discovered in mammals. Although it has been shown that lncRNAs are regulated differently among tissues and disease statuses, functions of these transcripts ...
Braun, Thomas +3 more
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Miniaturized fluorescent RNA dot blot method for rapid quantitation of gene expression
BMC Biotechnology, 2004 Background RNA dot blot hybridization is a commonly used technique for gene expression assays. However, membrane based RNA dot/slot blot hybridization is time consuming, requires large amounts of RNA, and is less suited for parallel assays of more than ...
Yadetie Fekadu +4 more
doaj +1 more source
Rapid, tunable, and multiplexed detection of RNA using convective array PCR
Communications Biology, 2023 Detection of RNA targets is typically achieved through RT-qPCR or RNAseq. RT-qPCR is rapid but limited in number and complexity of targets detected, while RNAseq is high-throughput but takes multiple days.
Andrew T. Sullivan +9 more
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Direct detection of Plasmodium female gametocyte-specific transcript s25 mRNA in blood by CLIP-PCR [PDF]
Jichu yixue yu linchuang, 2022 Objective To establish a molecular detection method for the Plasmodium female gametocyte. Methods Probes for capture and ligation probe-PCR (CLIP-PCR) were specific designed based on Plasmodium female gametocyte special RNA targets, which were the ...
YANG Jun-yuan, PIAN Hong-ru, YANG Ming-zhu, ZHENG Zhi
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Current Opinion in Structural Biology, 2019 RNA structure underpins many essential functions in biology. New chemical reagents and techniques for probing RNA structure in living cells have emerged in recent years. High-throughput, genome-wide techniques such as Structure-seq2 and DMS-MaPseq exploit nucleobase modification by dimethylsulfate (DMS) to obtain complete structuromes, and are ...
David Mitchell +2 more
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Osmium tetroxide as a probe of RNA structure [PDF]
RNA, 2017 Structured RNAs have a central role in cellular function. The capability of structured RNAs to adopt fixed architectural structures or undergo dynamic conformational changes contributes to their diverse role in the regulation of gene expression. Although numerous biophysical and biochemical tools have been developed to study structured RNAs, there is a
Jing Zhang +4 more
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Physico-chemical foundations underpinning microarray and next-generation sequencing experiments [PDF]
, 2013 Hybridization of nucleic acids on solid surfaces is a key process involved in high-throughput technologies such as microarrays and, in some cases, next-generation sequencing (NGS).
A. Buhot +70 more
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Human fibroblast interferon RNA transcripts of different sizes in poly(I).poly(C) induced cells [PDF]
, 1983 A
Content, Jean +3 more
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