Results 41 to 50 of about 616,337 (352)

THE CELLULAR RECEPTOR (CD4) OF THE HUMAN IMMUNODEFICIENCY VIRUS IS EXPRESSED ON NEURONS AND GLIAL CELLS IN HUMAN BRAIN [PDF]

, 1987
The peculiar tropism of the human immunodeficiency virus (HIV) for T helper lymphocytes can be explained by a specific interaction between the virus and the CD4 molecule on these cells (1, 2).
Funke, Ilona, Hahn, Alfred, Rieber, Ernst Peter, Riethmüller, Gert, Weiss, Elisabeth H.   +4 more
core   +1 more source

Tissue-specific expression of high-voltage-activated dihydropyridine-sensitive L-type calcium channels [PDF]

, 1991
The cloning of the cDNA for the α1 subunit of L-type calcium channels revealed that at least two genes (CaCh1 and CaCh2) exist which give rise to several splice variants.
Caffrey J. M., Catterall W. A., Ellis S. B., Glossmann H., Grabner M., Hofmann F., Hoshi T., Jay S. D., Jongh K. S., Kim H. S., Koch W. J., Koch W. J., Melton D. A., Olson E. N., Perez-Reyes E., Rampe D. E., Reuter H., Ruth P., Ruth P., Sambrook J., Sanger F., Schubert D., Shih H.-T., Snutch T. P., Tanabe T., Thomas P. S., Tsien R. W.   +26 more
core   +2 more sources

Northern Blot with IR Fluorescent Probes: Strategies for Probe Preparation

Bio-Protocol, 2019
Northern blot is a molecular biology technique that can detect, quantify, and determine the molecular weight of RNA. Recently, we published a protocol utilizing near-infrared (IR) fluorescent probes in Northern blot (irNorthern).
Christopher Fields, Peike Sheng, Bret Miller, Tianqi Wei, Mingyi Xie   +4 more
doaj   +1 more source

Optimising the analysis of transcript data using high density oligonucleotide arrays and genomic DNA-based probe selection [PDF]

, 2007
Background: Affymetrix GeneChip arrays are widely used for transcriptomic studies in a diverse range of species. Each gene is represented on a GeneChip array by a probe-set, consisting of up to 16 probe-pairs. Signal intensities across probe-pairs within
Broadley, Martin R., Graham, Neil S., Hammond, John P., May, Sean T., White, Philip J.   +4 more
core   +4 more sources

The T7-primer is a source of experimental bias and introduces variability between microarray platforms. [PDF]

PLoS ONE, 2008
Eberwine(-like) amplification of mRNA adds distinct 6-10 bp nucleotide stretches to the 5' end of amplified RNA transcripts. Analysis of over six thousand microarrays reveals that probes containing motifs complementary to these stretches are associated ...
Ron M Kerkhoven, Daoud Sie, Marja Nieuwland, Mike Heimerikx, Jorma De Ronde, Wim Brugman, Arno Velds   +6 more
doaj   +1 more source

Transcription-Based Amplified Colorimetric Thrombin Sensor Using Non-Crosslinking Aggregation of DNA-Modified Gold Nanoparticles

Sensors, 2021
Gold nanoparticles (AuNPs) have been employed as colorimetric biosensors due to the color difference between their dispersed (red) and aggregated (blue) states.
Yu Muto, Gen Hirao, Tamotsu Zako
doaj   +1 more source

Discovery of RNA-binding proteins and characterization of their dynamic responses by enhanced RNA interactome capture

Nature Communications, 2018
RNA interactome capture allows the detailed investigation of RNA-bound proteomes. Here the authors describe enhanced RNA-interactome capture using LNA-modified probes for increased sensitivity and specificity.
Joel I. Perez-Perri, Birgit Rogell, Thomas Schwarzl, Frank Stein, Yang Zhou, Mandy Rettel, Annika Brosig, Matthias W. Hentze   +7 more
doaj   +1 more source

Detection of NASBA amplified bacterial tmRNA molecules on SLICSel designed microarray probes

BMC Biotechnology, 2011
Background We present a comprehensive technological solution for bacterial diagnostics using tmRNA as a marker molecule. A robust probe design algorithm for microbial detection microarray is implemented.
Toome Kadri, Parkel Sven, Palta Priit, Glynn Barry, Kaplinski Lauris, Scheler Ott, Maher Majella, Barry Thomas, Remm Maido, Kurg Ants   +9 more
doaj   +1 more source

In vitro and in vivo expression of foreign genes by transmissible gastroenteritis coronavirus-derived minigenomes [PDF]

, 2002
A helper-dependent expression system based on transmissible gastroenteritis coronavirus (TGEV) has been developed using a minigenome of 3·9 kb (M39).
Alonso, S., Balasch, M., Enjuanes, L., Izeta, A., Moormann, R.J.M., Plana Duran, J., Reimann, I., Sola, I., Teifke, J.P.   +8 more
core   +3 more sources

Reciprocal control of viral infection and phosphoinositide dynamics

FEBS Letters, EarlyView.
Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
wiley   +1 more source