Nanoribbon-Based Electronic Detection of a Glioma-Associated Circular miRNA
Biosensors, 2021 Nanoribbon chips, based on “silicon-on-insulator” structures (SOI-NR chips), have been fabricated. These SOI-NR chips, whose surface was sensitized with covalently immobilized oligonucleotide molecular probes (oDNA probes), have been employed for the ...
Yuri D. Ivanov +18 more
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cDNA-RNA subtractive hybridization reveals increased expression of mycocerosic acid synthase in intracellular Mycobacterium bovis BCG. [PDF]
, 2001 Identifying genes that are differentially expressed by Mycobacterium bovis BCG after phagocytosis by macrophages will facilitate the understanding of the molecular mechanisms of host cell-intracellular pathogen interactions. To identify such genes a cDNA-
Centre Gunnels Wood +7 more
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Probe Generation Directly from Small Numbers of Cells for DNA Microarray Studies
BioTechniques, 2003 Recently, we described a technique that allows us to prepare probes for expression profiling from 0.5–1 μg RNA without template or signal amplification.
C.C. Xiang +6 more
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tmRNA - a novel high-copy-number RNA diagnostic target - its application for Staphylococcus aureus detection using real-time NASBA [PDF]
, 2009 A real-time nucleic acid sequence-based amplification assay, targeting tmRNA, was designed for the rapid identification of Staphylococcus aureus. The selectivity of the assay was confirmed against a panel of 76 Staphylococcus strains and species and 22 ...
Anonymous +9 more
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Development of a Chemiluminescence-Based Ribonuclease Protection Assay
BioTechniques, 2002 The ribonuclease protection assay (RPA) is a widely used method for the detection and quantification of specific mRNA transcripts in a complex mixture of total RNA or mRNA molecules. While exhibiting many advantages over other RNA detection methods, RPAs
Christopher Rosenau +2 more
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Quantification of virus-infected cells using RNA FISH-Flow
STAR Protocols, 2023 Summary: We present a protocol to detect cells that have been infected by RNA viruses. The method, RNA fluorescence in situ hybridization flow cytometry (RNA FISH-Flow), uses 48 fluorescently labeled DNA probes that hybridize in tandem to viral RNA.
Cody J. Warren +6 more
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The T7-primer is a source of experimental bias and introduces variability between microarray platforms. [PDF]
PLoS ONE, 2008 Eberwine(-like) amplification of mRNA adds distinct 6-10 bp nucleotide stretches to the 5' end of amplified RNA transcripts. Analysis of over six thousand microarrays reveals that probes containing motifs complementary to these stretches are associated ...
Ron M Kerkhoven +6 more
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Nature Communications, 2018 RNA interactome capture allows the detailed investigation of RNA-bound proteomes. Here the authors describe enhanced RNA-interactome capture using LNA-modified probes for increased sensitivity and specificity.
Joel I. Perez-Perri +7 more
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Sensors, 2021 Gold nanoparticles (AuNPs) have been employed as colorimetric biosensors due to the color difference between their dispersed (red) and aggregated (blue) states.
Yu Muto, Gen Hirao, Tamotsu Zako
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Multiple changes in gene expression in chronic human Achilles tendinopathy [PDF]
, 2002 Atlas™ cDNA cell interaction arrays (CLONTECH) were used to examine degenerate tissue from four patients with Achilles tendon disorders, in order to identify changes in expression of genes important in cell–cell and cell–matrix interactions. The greatest
Armitage +22 more
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