Results 61 to 70 of about 587,888 (232)
Frontiers in MicrobiologyLymphatic filariasis is caused by parasitic nematodes and is a leading cause of disability worldwide. Many filarial worms contain the bacterium Wolbachia as an obligate endosymbiont.
Lindsey J. Cantin +3 more
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Development of peptide nucleic acid probes for detection of the HER2 oncogene. [PDF]
PLoS ONE, 2013 Peptide nucleic acids (PNAs) have gained much interest as molecular recognition tools in biology, medicine and chemistry. This is due to high hybridization efficiency to complimentary oligonucleotides and stability of the duplexes with RNA or DNA.
Belhu Metaferia +13 more
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SARS-CoV-2 Mutations and Variants May Muddle the Sensitivity of COVID-19 Diagnostic Assays
Microorganisms, 2022 The performance of diagnostic polymerase chain reaction (PCR) assays can be impacted by SARS-CoV-2 variability as this is dependent on the full complementarity between PCR primers/probes and viral target templates.
Mohammad Alkhatib +5 more
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In-line probing of RNA G-quadruplexes
Methods, 2013 Although the majority of the initial G-quadruplex studies were performed on DNA molecules, there currently exists a rapidly growing interest in the investigation of those formed in RNA molecules that possess high potential of acting as gene expression regulatory elements.
Beaudoin, Jean-Denis +2 more
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Scientific Reports, 2017 Analysis of RNA dynamics and localization at the single-molecule level in living cells has been predominantly achieved by engineering target RNAs with large insertions of tandem repeat sequences that are bound by protein-based or oligonucleotide-based ...
Mingming Chen +7 more
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Fast-exchanging spirocyclic rhodamine probes for aptamer-based super-resolution RNA imaging
Nature Communications, 2023 Live-cell RNA imaging with high spatial and temporal resolution remains a major challenge. Here we report the development of RhoBAST:SpyRho, a fluorescent light-up aptamer (FLAP) system ideally suited for visualizing RNAs in live or fixed cells with ...
Daniel Englert +10 more
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Techniques for RNA in vivo imaging in plants [PDF]
, 2014 Since the discovery of small RNAs and RNA silencing, RNA biology has taken a centre stage in cell and developmental biology. Small RNAs, but also mRNAs and other types of cellular and viral RNAs are processed at specific subcellular localizations.
Tilsner, Jens
core +1 more source
RNA Isolation and Northern Blot Analysis
Bio-Protocol, 2014 The northern blot is a technique used in molecular biology research to study gene expression by detection of RNA in a sample. With northern blotting it is possible to observe particular gene expression levels during differentiation, morphogenesis, as ...
Ying Liao +5 more
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psiCLIP reveals dynamic RNA binding by DEAH-box helicases before and after exon ligation
Nature Communications, 2021 ATP-dependent helicases remodel the spliceosome and proofread splice site recognition. A new method – Purified Spliceosome iCLIP (psiCLIP) – probes protein-RNA interactions in defined spliceosome complexes to reveal how the helicases Prp16 and Prp22 ...
Lisa M. Strittmatter +9 more
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Subcellular partitioning of MRP RNA assessed by ultrastructural and biochemical analysis. [PDF]
, 1994 A small RNA encoded within the nucleus is an essential subunit of a RNA processing endonuclease (RNase MRP) hypothesized to generate primers for mitochondrial DNA replication from the heavy strand origin of replication.
Gonzalez, M +6 more
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