Results 71 to 80 of about 955,921 (334)

Evolutionary interplay between viruses and R‐loops

FEBS Letters, EarlyView.
Viruses interact with specialized nucleic acid structures called R‐loops to influence host transcription, epigenetic states, latency, and immune evasion. This Perspective examines the roles of R‐loops in viral replication, integration, and silencing, and how viruses co‐opt or avoid these structures.
Zsolt Karányi, Zita Képes, Zoltán Szabó, Eszter Csoma, Lóránt Székvölgyi   +4 more
wiley   +1 more source

Decoding Non-coding Variants: Recent Approaches to Studying Their Role in Gene Regulation and Human Diseases

Frontiers in Bioscience-Scholar
Genome-wide association studies (GWAS) have mapped over 90% of disease- and quantitative-trait-associated variants within the non-coding genome. Non-coding regulatory DNA (e.g., promoters and enhancers) and RNA (e.g., 5′ and 3′ UTRs and splice sites) are
Edwin G. Peña-Martínez, José A. Rodríguez-Martínez   +1 more
doaj   +1 more source

Genome-wide Mapping of 5′-monophosphorylated Ends of Mammalian Nascent RNA Transcripts

Bio-Protocol, 2023
In eukaryotic cells, RNA biogenesis generally requires processing of the nascent transcript as it is being synthesized by RNA polymerase. These processing events include endonucleolytic cleavage, exonucleolytic trimming, and splicing of the growing ...
Michael Cortázar, Nova Fong, David Bentley   +2 more
doaj   +1 more source

Significance of the dissociation of Dna2 by flap endonuclease 1 to Okazaki fragment processing in Saccharomyces cerevisiae [PDF]

, 2009
Okazaki fragments are initiated by short RNA/DNA primers, which are displaced into flap intermediates for processing. Flap endonuclease 1 (FEN1) and Dna2 are responsible for flap cleavage.
Ayyagari, Bae, Bae, Bae, Budd, Budd, Budd, Budd, Budd, Budd, Burgers, Burgers, Choe, Garg, Gordenin, Henricksen, Hohl, Jason A. Stewart, Judith L. Campbell, Kang, Kao, Kao, Kao, Kim, Kunkel, Liao, Liu, Liu, Masuda-Sasa, Masuda-Sasa, Murante, Robert A. Bambara, Rossi, Rossi, Rossi, Stewart, Stewart, Subramanian, Tomita, Vernon, Weitao, Wold, Zhu   +42 more
core   +2 more sources

Purification tags markedly affect self‐aggregation of CPEB3

FEBS Letters, EarlyView.
Although recombinant proteins are used to study protein aggregation in vitro, uncleaved tags can interfere with accurate interpretation. Our findings demonstrate that His₆‐GFP and His₁₂ tags significantly affect liquid droplet and amyloid fibril formation in the intrinsically disordered region (IDR) of mouse cytoplasmic polyadenylation element‐binding ...
Harunobu Saito, Yujin Lee, Motoharu Ueno, Naotaka Sekiyama, Masatomo So, Ayako Furukawa, Kenji Sugase   +6 more
wiley   +1 more source

A snoRNA modulates mRNA 3' end processing and regulates the expression of a subset of mRNAs. [PDF]

, 2017
mRNA 3' end processing is an essential step in gene expression. It is well established that canonical eukaryotic pre-mRNA 3' processing is carried out within a macromolecular machinery consisting of dozens of trans-acting proteins. However, it is unknown
Ding, Junjun, Guo, Yibin, Huang, Chunliu, Huang, Shanshan, Huang, Weijun, Huang, Xi, Jia, Jie, Ming, Siqi, Shi, Junjie, Shi, Yongsheng, Wu, Xingui, Xiang, Andy Peng, Yao, Chengguo, Zhang, Rui, Zhao, Wei   +14 more
core   +1 more source

Insights into pegRNA design from editing of the cardiomyopathy‐associated phospholamban R14del mutation

FEBS Letters, EarlyView.
This study reveals how prime editing guide RNA (pegRNA) secondary structure and reverse transcriptase template length affect prime editing efficiency in correcting the phospholamban R14del cardiomyopathy‐associated mutation. Insights support the design of structurally optimized enhanced pegRNAs for precise gene therapy.
Bing Yao, Qiangbing Yang, Christian J.B. Snijders Blok, Mark A. Daniels, Pieter A. Doevendans, Raymond Schiffelers, Joost P.G. Sluijter, Zhiyong Lei   +7 more
wiley   +1 more source

Single‐cell insights into the role of T cells in B‐cell malignancies

FEBS Letters, EarlyView.
Single‐cell technologies have transformed our understanding of T cell–tumor cell interactions in B‐cell malignancies, revealing new T‐cell subsets, functional states, and immune evasion mechanisms. This Review synthesizes these findings, highlighting the roles of T cells in pathogenesis, progression, and therapy response, and underscoring their ...
Laura Llaó‐Cid
wiley   +1 more source

Human Telomerase RNA Processing and Quality Control

Cell Reports, 2015
The non-coding RNA subunit of telomerase provides the template for telomerase activity. In diverse fungi, 3′ end processing of telomerase RNA involves a single cleavage by the spliceosome.
Chi-Kang Tseng, Hui-Fang Wang, Allison M. Burns, Morgan R. Schroeder, Martina Gaspari, Peter Baumann   +5 more
doaj   +1 more source

The Sm complex is required for the processing of non-coding RNAs by the exosome. [PDF]

PLoS ONE, 2013
A key question in the field of RNA regulation is how some exosome substrates, such as spliceosomal snRNAs and telomerase RNA, evade degradation and are processed into stable, functional RNA molecules.
Sarah Coy, Adam Volanakis, Sneha Shah, Lidia Vasiljeva   +3 more
doaj   +1 more source