Results 81 to 90 of about 938,495 (334)

A cellular system to study responses to a collision between the transcription complex and a protein‐bound nick in the DNA template

FEBS Letters, EarlyView.
We present the cellular transcription‐coupled Flp‐nick system allowing the introduction of a Top1‐mimicking cleavage complex (Flpcc) at a Flp recognition target site within a controllable LacZ gene. LacZ transcription leads to the collision of RNA polymerase II (RNAPII) with Flpcc, and this causes RNAPII stalling, ubiquitination, and degradation.
Petra Herring, Morten Roedgaard, Camilla Myrup Holst, Helene Christensen, Birgitta R. Knudsen, Lotte Bjergbaek, Anni Hangaard Andersen   +6 more
wiley   +1 more source

Human Telomerase RNA Processing and Quality Control

Cell Reports, 2015
The non-coding RNA subunit of telomerase provides the template for telomerase activity. In diverse fungi, 3′ end processing of telomerase RNA involves a single cleavage by the spliceosome.
Chi-Kang Tseng, Hui-Fang Wang, Allison M. Burns, Morgan R. Schroeder, Martina Gaspari, Peter Baumann   +5 more
doaj   +1 more source

SSB-1 of the yeast Saccharomyces cerevisiae is a nucleolar-specific, silver-binding protein that is associated with the snR10 and snR11 small nuclear RNAs [PDF]

, 1990
SSB-1, the yeast single-strand RNA-binding protein, is demonstrated to be a yeast nucleolar-specific, silver-binding protein. In double-label immunofluorescence microscopy experiments antibodies to two other nucleolar proteins, RNA Pol I 190-kD and ...
Abelson, John, Campbell, Judith, Clark, Michael W., Yip, Man Lun R.   +3 more
core   +2 more sources

The discovery potential of RNA processing profiles [PDF]

Nucleic Acids Research, 2016
AbstractSmall non-coding RNAs are highly abundant molecules that regulate essential cellular processes and are classified according to sequence and structure. Here we argue that read profiles from size-selected RNA sequencing capture the post-transcriptional processing specific to each RNA family, thereby providing functional information independently ...
Joan Pallarès-Albanell, Eduardo Eyras, Eduardo Eyras, Amadís Pagès, Ivan Dotu, Eulàlia Martí, Roderic Guigó   +6 more
openaire   +6 more sources

Interaction vesicles as emerging mediators of host‐pathogen molecular crosstalk and their implications for infection dynamics

FEBS Letters, EarlyView.
Interaction extracellular vesicles (iEVs) are hybrid vesicles formed through host‐pathogen communication. They facilitate immune evasion, transfer pathogens' molecules, increase host cell uptake, and enhance virulence. This Perspective article illustrates the multifunctional roles of iEVs and highlights their emerging relevance in infection dynamics ...
Bruna Sabatke, Izadora Volpato Rossi, Marcel I. Ramirez   +2 more
wiley   +1 more source

Mitochondrial RNA Granules Are Centers for Posttranscriptional RNA Processing and Ribosome Biogenesis

Cell Reports, 2015
Cytoplasmic RNA granules play a central role in mRNA metabolism, but the importance of mitochondrial RNA granules remains relatively unexplored. We characterized their proteome and found that they contain a large toolbox of proteins dedicated to RNA ...
Hana Antonicka, Eric A. Shoubridge
doaj   +1 more source

A snoRNA modulates mRNA 3' end processing and regulates the expression of a subset of mRNAs. [PDF]

, 2017
mRNA 3' end processing is an essential step in gene expression. It is well established that canonical eukaryotic pre-mRNA 3' processing is carried out within a macromolecular machinery consisting of dozens of trans-acting proteins. However, it is unknown
Ding, Junjun, Guo, Yibin, Huang, Chunliu, Huang, Shanshan, Huang, Weijun, Huang, Xi, Jia, Jie, Ming, Siqi, Shi, Junjie, Shi, Yongsheng, Wu, Xingui, Xiang, Andy Peng, Yao, Chengguo, Zhang, Rui, Zhao, Wei   +14 more
core   +1 more source

STAT3 expression is reduced in cardiac pericytes in HFpEF and its loss reduces cellular adhesion and induces pericyte senescence

FEBS Letters, EarlyView.
Heart failure with preserved ejection fraction (HFpEF) accounts for half of the heart failure cases. It is characterised by microvascular dysfunction, associated with reduced pericyte coverage and diminished STAT3 expression in pericytes. Loss of STAT3 impairs pericyte adhesion, promotes senescence, and activates a pro‐fibrotic gene program.
Leah Rebecca Vanicek, Ariane Fischer, Mariano Ruz Jurado, Anita Tamiato, Tara Procida‐Kowalski, Jochen Wilhelm, Dennis Hecker, Maximilian Merten, Felicitas Escher, Badder Kattih, Valentina Puntmann, David John, Marcel H. Schulz, Eike Nagel, Stefanie Dimmeler, Guillermo Luxán   +15 more
wiley   +1 more source

Decoding Non-coding Variants: Recent Approaches to Studying Their Role in Gene Regulation and Human Diseases

Frontiers in Bioscience-Scholar
Genome-wide association studies (GWAS) have mapped over 90% of disease- and quantitative-trait-associated variants within the non-coding genome. Non-coding regulatory DNA (e.g., promoters and enhancers) and RNA (e.g., 5′ and 3′ UTRs and splice sites) are
Edwin G. Peña-Martínez, José A. Rodríguez-Martínez   +1 more
doaj   +1 more source

Synthetic RNA modules for fine-tuning gene expression levels in yeast by modulating RNase III activity [PDF]

, 2011
The design of synthetic gene networks requires an extensive genetic toolbox to control the activities and levels of protein components to achieve desired cellular functions.
Alper, Alper, Alper, Anderson, Andrew H. Babiskin, Babiskin, Babiskin, Basu, Beisel, Callura, Caponigro, Carrier, Chanfreau, Chanfreau, Chao, Christina D. Smolke, Danin-Kreiselman, Elela, Ellis, Elowitz, Gardner, Ge, Gietz, Hawkins, Hawkins, Isaacs, Jensen, Jin, Jones, Keiler, Lamontagne, Lamontagne, Lamontagne, Lamontagne, Lamontagne, Lamontagne, Larose, Lautz, Lavoie, Lee, Louis, Lutz, Mateus, Misra, Nevoigt, Ng, Nguyen, Ostergaard, Paradise, Pfleger, Ro, Salis, Sambrook, Szczebara, Vasudevan, Veen, Win, Wu, Wu, Zhou, Zhou, Zhu, Zhu   +62 more
core   +3 more sources