Results 31 to 40 of about 540 (209)
HIV-1 spliced RNAs display transcription start site bias [PDF]
RNA, 2020 Human immunodeficiency virus type 1 (HIV-1) transcripts have three fates: to serve as genomic RNAs, unspliced mRNAs, or spliced subgenomic mRNAs. Recent structural studies have shown that sequences near the 5′ end of HIV-1 RNA can adopt at least two alternate three-dimensional conformations, and that these structures dictate genome versus unspliced ...
Jackie M. Esquiaqui +3 more
openaire +2 more sources
Assessing the impacts of various factors on circular RNA reliability
Life Science Alliance, 2023 This study systematically assesses the impacts of numerous factors related to circRNA identification, conservation, biogenesis, and function on circRNA reliability, providing a useful guideline and an important resource for selecting high-confidence ...
Trees-Juen Chuang +2 more
doaj +1 more source
Probing of the spliceosome with site-specifically derivatized 5′ splice site RNA oligonucleotides
RNA, 1998 We have developed a site-specific chemical modification technique to incorporate a photoreactive azidophenacyl (APA) group at designated internal positions along the RNA phosphodiester backbone. Using this technique, we have analyzed interactions of the 5' splice site (5'SS) RNA within the spliceosome.
M, Sha, T, Levy, P, Kois, M M, Konarska
openaire +3 more sources
iScience, 2019 Summary: RNA splicing is related to many human diseases; however, lack of efficient genetic approaches to modulate splicing has prevented us from dissecting their functions in human diseases.
Shisheng Huang +11 more
doaj +1 more source
Noisy splicing drives mRNA isoform diversity in human cells. [PDF]
PLoS Genetics, 2010 While the majority of multiexonic human genes show some evidence of alternative splicing, it is unclear what fraction of observed splice forms is functionally relevant.
Joseph K Pickrell +3 more
doaj +1 more source
Microbiology Independent Research Journal, 2021 The influenza A virus genome consists of eight segments of negative-sense RNA that encode up to 18 proteins. During the process of viral replication, positive-sense (+)RNA (cRNA) or messenger RNA (mRNA) is synthesized.
Irina L. Baranovskaya +4 more
doaj +1 more source
Genome-wide analyses supported by RNA-Seq reveal non-canonical splice sites in plant genomes
BMC Genomics, 2018 Background Most eukaryotic genes comprise exons and introns thus requiring the precise removal of introns from pre-mRNAs to enable protein biosynthesis. U2 and U12 spliceosomes catalyze this step by recognizing motifs on the transcript in order to remove
Boas Pucker, Samuel F. Brockington
doaj +1 more source
BMC Research Notes, 2017 Objective The Arabidopsis thaliana Niederzenz-1 genome sequence was recently published with an ab initio gene prediction. In depth analysis of the predicted gene set revealed some errors involving genes with non-canonical splice sites in their introns ...
Boas Pucker +2 more
doaj +1 more source
PLoS Genetics, 2022 Pre-mRNA splicing is an essential step of eukaryotic gene expression carried out by a series of dynamic macromolecular protein/RNA complexes, known collectively and individually as the spliceosome.
Jessie M N G L Suzuki +5 more
doaj +1 more source
An extended catalogue of tandem alternative splice sites in human tissue transcriptomes.
PLoS Computational Biology, 2021 Tandem alternative splice sites (TASS) is a special class of alternative splicing events that are characterized by a close tandem arrangement of splice sites. Most TASS lack functional characterization and are believed to arise from splicing noise. Based
Aleksei Mironov +4 more
doaj +1 more source