RNA Stability Measurements Using RT-qPCR in Arabidopsis Seedlings [PDF]
Bio-Protocol, 2020 Steady-state mRNA levels are determined by both the rates of transcription and degradation. Regulation of mRNA stability and/or degradation are key factors that can significantly affect mRNA levels and its biological functions.
Tianran Jia, Brandon Le
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Post-mortem Interval and Its Relation with the RNA Degradation in the Dental Pulp in Submerged Teeth
Arab Journal of Forensic Sciences & Forensic Medicine, 2021 Post-mortem interval is the time between death and the discovery of the body or human remains. Teeth are the most resistant structures of the human body, able to withstand extreme conditions such as high temperature, humidity, and post-mortem degradation.
Bruna S. Borges +3 more
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Incoherent dual regulation by a SAM-II riboswitch controlling translation at a distance
RNA Biology, 2022 In Sinorhizobium meliloti, the methionine biosynthesis genes metA and metZ are preceded by S-adenosyl-L-methionine (SAM) riboswitches of the SAM-II class. Upon SAM binding, structural changes in the metZ riboswitch were predicted to cause transcriptional
Robina Scheuer +5 more
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Arginine multivalency stabilizes protein/RNA condensates [PDF]
Protein Science, 2021 Abstract Biomolecular condensates assembled through liquid–liquid phase separation (LLPS) of proteins and RNAs are currently recognized to play an important role in cellular organization. Their assembly depends on the formation of a network of transient, multivalent interactions between flexible scaffold biomolecules.
Matteo Paloni +2 more
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Characterizing RNA stability genome-wide through combined analysis of PRO-seq and RNA-seq data
BMC Biology, 2021 Background The concentrations of distinct types of RNA in cells result from a dynamic equilibrium between RNA synthesis and decay. Despite the critical importance of RNA decay rates, current approaches for measuring them are generally labor-intensive ...
Amit Blumberg +8 more
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Predicting 3D structure and stability of RNA pseudoknots in monovalent and divalent ion solutions. [PDF]
PLoS Computational Biology, 2018 RNA pseudoknots are a kind of minimal RNA tertiary structural motifs, and their three-dimensional (3D) structures and stability play essential roles in a variety of biological functions.
Ya-Zhou Shi +4 more
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Repression of PUM1-mediated mRNA decay activates translesion synthesis after DNA damage
Molecular & Cellular Oncology, 2020 Biological roles of Pumilio1 (PUM1) in ubiquitous cells remain unclear. Here we identify 48 degrading target mRNAs by combined analysis of transcriptome-wide mRNA stabilities and the binding of mRNAs.
Toshimichi Yamada +2 more
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Stability of SARS-CoV-2 RNA in Nonsupplemented Saliva
Emerging Infectious Diseases, 2021 The expense of saliva collection devices designed to stabilize severe acute respiratory syndrome coronavirus 2 RNA is prohibitive to mass testing. However, virus RNA in nonsupplemented saliva is stable for extended periods and at elevated temperatures ...
Isabel M. Ott +23 more
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Borate Minerals and RNA Stability [PDF]
Polymers, 2010 The abiotic origin of genetic polymers faces two major problems: a prebiotically plausible polymerization mechanism and the maintenance of their polymerized state outside a cellular environment. The stabilizing action of borate on ribose having been reported, we have explored the possibility that borate minerals stabilize RNA.
Cristina Cossetti +3 more
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Frozen section histopathology and preanalytical factors affecting nucleic acid integrity in biobanked fresh-frozen human cancer tissues [PDF]
Journal of Pathology and Translational MedicineBackground In this study, we evaluated the effects of storage duration and ischemic time on nucleic acid quality of fresh-frozen tissue (FFT) from colon adenocarcinoma (COAD), hepatocellular carcinoma (HCC), and renal cell carcinoma (RCC) collected at ...
Soungeun Kim +4 more
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