Results 21 to 30 of about 511,041 (280)

Borate Minerals and RNA Stability [PDF]

Polymers, 2010
The abiotic origin of genetic polymers faces two major problems: a prebiotically plausible polymerization mechanism and the maintenance of their polymerized state outside a cellular environment. The stabilizing action of borate on ribose having been reported, we have explored the possibility that borate minerals stabilize RNA.
Cristina Cossetti, Claudia Crestini, Raffaele Saladino, DI MAURO, Ernesto   +3 more
openaire   +5 more sources

Repression of PUM1-mediated mRNA decay activates translesion synthesis after DNA damage

Molecular & Cellular Oncology, 2020
Biological roles of Pumilio1 (PUM1) in ubiquitous cells remain unclear. Here we identify 48 degrading target mRNAs by combined analysis of transcriptome-wide mRNA stabilities and the binding of mRNAs.
Toshimichi Yamada, Xiaoning Sun, Nobuyoshi Akimitsu   +2 more
doaj   +1 more source

Stability of SARS-CoV-2 RNA in Nonsupplemented Saliva

Emerging Infectious Diseases, 2021
The expense of saliva collection devices designed to stabilize severe acute respiratory syndrome coronavirus 2 RNA is prohibitive to mass testing. However, virus RNA in nonsupplemented saliva is stable for extended periods and at elevated temperatures ...
Isabel M. Ott, Madison S. Strine, Anne E. Watkins, Maikel Boot, Chaney C. Kalinich, Christina A. Harden, Chantal B.F. Vogels, Arnau Casanovas-Massana, Adam J. Moore, M. Catherine Muenker, Maura Nakahata, Maria Tokuyama, Allison Nelson, John Fournier, Santos Bermejo, Melissa Campbell, Rupak Datta, Charles S. Dela Cruz, Shelli F. Farhadian, Albert I. Ko, Akiko Iwasaki, Nathan D. Grubaugh, Craig B. Wilen, Anne L. Wyllie   +23 more
doaj   +1 more source

Frozen section histopathology and preanalytical factors affecting nucleic acid integrity in biobanked fresh-frozen human cancer tissues [PDF]

Journal of Pathology and Translational Medicine
Background In this study, we evaluated the effects of storage duration and ischemic time on nucleic acid quality of fresh-frozen tissue (FFT) from colon adenocarcinoma (COAD), hepatocellular carcinoma (HCC), and renal cell carcinoma (RCC) collected at ...
Soungeun Kim, Jaewon Kang, Boyeon Kim, Yoonjin Kwak, Hye Seung Lee   +4 more
doaj   +1 more source

Hybridization Properties of RNA Containing 8-Methoxyguanosine and 8-Benzyloxyguanosine. [PDF]

PLoS ONE, 2015
Modified nucleobase analogues can serve as powerful tools for changing physicochemical and biological properties of DNA or RNA. Guanosine derivatives containing bulky substituents at 8 position are known to adopt syn conformation of N-glycoside bond.
Daniel Sylwester Baranowski, Weronika Kotkowiak, Ryszard Kierzek, Anna Pasternak   +3 more
doaj   +1 more source

The emerging role of RNA modifications in the regulation of mRNA stability

Experimental and Molecular Medicine, 2020
Gene activity: the significance of RNA modification Messenger RNA molecules play their key role in directing the manufacture of proteins via translating the genetic information in DNA.
Sung Ho Boo, Yoon Ki Kim
doaj   +1 more source

Stability of RNA virus attenuation approaches [PDF]

Vaccine, 2011
The greatest risk from live-attenuated vaccines is reversion to virulence. Particular concerns arise for RNA viruses, which exhibit high mutation frequencies. We examined the stability of 3 attenuation strategies for the alphavirus, Venezuelan equine encephalitis virus (VEEV): a traditional, point mutation-dependent attenuation approach exemplified by ...
Joan L, Kenney, Sara M, Volk, Jyotsna, Pandya, Eryu, Wang, Xiaodong, Liang, Scott C, Weaver   +5 more
openaire   +2 more sources

Immunostimulating capacities of stabilized RNA molecules [PDF]

European Journal of Immunology, 2004
AbstractSince direct injection of naked mRNA induces an immune response, we tested the capacity of RNA to signal danger. We show here that mRNA molecules that are protected from immediate degradation either through interaction with cationic proteins (trans protection) or through chemical modification of the phosphodiester backbone (phosphorothioate RNA;
Birgit, Scheel, Sybilla, Braedel, Jochen, Probst, Jean-Philippe, Carralot, Hermann, Wagner, Hansjorg, Schild, Günther, Jung, Hans-Georg, Rammensee, Steve, Pascolo   +8 more
openaire   +2 more sources

Salt-Dependent RNA Pseudoknot Stability: Effect of Spatial Confinement

Frontiers in Molecular Biosciences, 2021
Macromolecules, such as RNAs, reside in crowded cell environments, which could strongly affect the folded structures and stability of RNAs. The emergence of RNA-driven phase separation in biology further stresses the potential functional roles of ...
Chenjie Feng, Ya-Lan Tan, Yu-Xuan Cheng, Ya-Zhou Shi, Zhi-Jie Tan   +4 more
doaj   +1 more source

A tudor domain protein, SIMR-1, promotes siRNA production at piRNA-targeted mRNAs in C. elegans

eLife, 2020
piRNAs play a critical role in the regulation of transposons and other germline genes. In Caenorhabditis elegans, regulation of piRNA target genes is mediated by the mutator complex, which synthesizes high levels of siRNAs through the activity of an RNA ...
Kevin I Manage, Alicia K Rogers, Dylan C Wallis, Celja J Uebel, Dorian C Anderson, Dieu An H Nguyen, Katerina Arca, Kristen C Brown, Ricardo J Cordeiro Rodrigues, Bruno FM de Albuquerque, René F Ketting, Taiowa A Montgomery, Carolyn Marie Phillips   +12 more
doaj   +1 more source