Results 91 to 100 of about 541,018 (257)

Next-generation sequencing of dsRNA is greatly improved by treatment with the inexpensive denaturing reagent DMSO. [PDF]

, 2019
dsRNA is the genetic material of important viruses and a key component of RNA interference-based immunity in eukaryotes. Previous studies have noted difficulties in determining the sequence of dsRNA molecules that have affected studies of immune function
Delwart, Eric, Díaz-Muñoz, Samuel L, Wilcox, Alexander H   +2 more
core  

Delivery of Islatravir via High Drug‐Load, Long‐acting Microarray Patches for the Prevention or Treatment of Human Immunodeficiency Virus

Advanced Healthcare Materials, Volume 14, Issue 7, March 14, 2025.
Islatravir‐loaded dissolving microarray patches (MAPs) provide a minimally invasive, long‐acting solution for HIV‐1 prevention and treatment. In rats, sustained islatravir delivery lasts up to three months, while in minipigs, efficacious plasma levels are detected for six days.
Qonita Kurnia Anjani, Ashley R. Johnson, Akmal H. Sabri, Ryan Lutz, Steven Tignor, Jeanine Ballard, Nathan Rudd, Li Zhao, Lalitkumar K. Vora, Stephanie E. Barrett, Angela Wagner, Ryan F. Donnelly   +11 more
wiley   +1 more source

Recent Applications of Mesoporous Silica Nanoparticles in Gene Therapy

Advanced Healthcare Materials, EarlyView.
The review summarizes the synthesis of mesoporous silica nanoparticles (MSNs) with modifiable surface properties, functionalization strategies, mechanism of therapeutic payload release, and current applications in gene therapy, focusing on their capabilities in the targeted delivery of therapeutic nucleic acids, CRISPR‐Cas systems, and other genetic ...
Tamanna Binte Huq, Punnya Anil Kumar Jeeja, Sudip Kumar Dam, Sabya Sachi Das, Juan L. Vivero‐Escoto   +4 more
wiley   +1 more source

Systematic Comparison of Commercial Uranyl‐Alternative Stains for Negative‐ and Positive‐Staining Transmission Electron Microscopy of Organic Specimens

Advanced Healthcare Materials, EarlyView.
Negative‐ and positive‐staining TEM is essential for rapid nanometer‐resolution characterization of organic specimens ranging from nanoparticles to cells. Uranyl salts are widely used negative‐/positive‐stains but are radioactive and highly toxic to users and the environment.
Vera M. Kissling, Stephanie Eitner, Davide Bottone, Gea Cereghetti, Peter Wick   +4 more
wiley   +1 more source

Nanomaterial‐Enhanced Biosensing: Mechanisms and Emerging Applications

Advanced Healthcare Materials, EarlyView.
Nanomaterial integration transforms biosensor capabilities through enhanced signal transduction, sensitivity, and selectivity. This review analyzes how nanoscale materials—from nanoparticles to nanosheets—leverage unique physicochemical properties to revolutionize electrochemical, optical, and electrical biosensing.
Younghak Cho, Yunyoung Choi, Yerim Jang, Hyejeong Seong   +3 more
wiley   +1 more source

Sindbis virus proteins nsP1 and nsP2 contain homology to nonstructural proteins from several RNA plant viruses [PDF]

, 1985
Although the genetic organization of tobacco mosaic virus (TMV) differs considerably from that of the tripartite viruses (alfalfa mosaic virus [AlMV] and brome mosaic virus [BMV]), all of these RNA plant viruses share three domains of homology among ...
Ahlquist, Paul, Haseloff, James, Rice, Charles M., Strauss, Ellen G., Strauss, James H., Zimmern, David   +5 more
core   +1 more source

Rational Design of Unsaturated, Thioether Ionizable Lipids for Enhanced In Vivo mRNA Delivery

Advanced Healthcare Materials, EarlyView.
Lipid nanoparticles (LNPs) have rapidly become the delivery system of choice for RNA therapeutics. Here, a rational design approach is taken to improve the effectiveness of LNPs based on an ionizable thioether lipid library. The incorporation of unsaturated tails and bulkier, hydrophobic head groups significantly enhances their functionality and ...
Eleni Samaridou, Johanna Simon, Moritz Beck‐Broichsitter, Gary Davidson, Pavel A. Levkin   +4 more
wiley   +1 more source

Rational Design of Metal–Organic Frameworks for Pancreatic Cancer Therapy: from Machine Learning Screening to In Vivo Efficacy

Advanced Materials, EarlyView.
This work explores the MOF landscape to select a single, optimal candidate for successfully delivering cancer drugs (gemcitabine, paclitaxel, SN‐38) into tough pancreatic tumors. Machine learning and simulations guide this search, demonstrating colloidal stability, excellent biocompatibility, cellular uptake, and sustained release.
Francesca Melle, Dhruv Menon, João Conniot, Jon Ostolaza‐Paraiso, Sergio Mercado, Jhenifer Oliveira, Xu Chen, Bárbara B. Mendes, João Conde, David Fairen‐Jimenez   +9 more
wiley   +1 more source

Engineering CAR‐T Therapeutics for Enhanced Solid Tumor Targeting

Advanced Materials, EarlyView.
CART cell therapy has proven effective for blood cancers but struggles with solid tumors due to diverse antigens and complex environments. Recent efforts focus on improving CAR design and validation platforms. Advances in protein engineering, machine learning, and organoid systems aim to enhance CAR‐T therapy against solid tumors.
Danqing Zhu, Won Joon Kim, Hyunjin Lee, Xiaoping Bao, Pilnam Kim   +4 more
wiley   +1 more source

Micro‐ and Nano‐Bots for Infection Control

Advanced Materials, EarlyView.
This review presents a strategic vision for integrating micro‐ and nanobots in the pipeline for infection diagnosis, prevention, and treatment. To develop these robots as a practical solution for infection management, their design principles are clarified based on their propulsion mechanisms and then categorized infection management domains based on ...
Azin Rashidy Ahmady, Shadman Khan, Hong Han, Wei Gao, Zeinab Hosseinidoust, Tohid F. Didar   +5 more
wiley   +1 more source